Keywords:
Glycoconjugates-Analysis-Laboratory manuals.
;
Electronic books.
Description / Table of Contents:
This work covers methodologies for plant and animal glycoconjugate analysis. It details mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids and new physical methods, o-glycosylation characterization, chromophore and fluorophore labelling of oligosaccharides, separations, exoglycosidases and mapping, and plant glycobiology.
Type of Medium:
Online Resource
Pages:
1 online resource (665 pages)
Edition:
1st ed.
ISBN:
9781482225860
URL:
https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=5378985
DDC:
572/.567
Language:
English
Note:
Cover -- Half Title -- Title Page -- Copyright Page -- Preface -- Table of Contents -- Contributors -- Part I: Mass Spectrometry and NMR -- 1: New Frontiers in Nuclear Magnetic Resonance Spectroscopy: Use of the Nano*NMR Probe for the Analysis of Microgram Quantities of Complex Carbohydrates -- 2: Analysis of Anionic Glycoconjugates by Delayed Extraction Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry -- 3: Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Oligosaccharides Separated by High pH Anion-Exchange Chromatography -- 4: The Fragmentation Behavior of Glycopeptides Using the Post-Source Decay Technique and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry -- 5: Application of Electrospray Ionization Mass Spectrometry to Unusual Phosphorylated Oligosaccharides of Trypanosoma cruzi -- 6: Analysis of Carbohydrate-Protein Interactions by Mass Spectrometry -- 7: Mycobacterial Lipoarabinomannans: Structural and Tagging Studies -- Part II: Glycolipids and New Physical Methods -- 8: Microscale Analysis of Glycosphingolipids by Thin-Layer Chromatography Blotting -- 9: High-Performance Liquid Chromatography of Gangliosides -- 10: A Sensitive Cell Suspension Enzyme-Linked Immunosorbent Assay Measuring the Density of Cell Surface Ganglioside and Carbohydrate Antigens -- 11: Scattering Techniques for the Study of Aggregative Properties of Gangliosides -- 12: Imaging Carbohydrate Polymers with Noncontact Mode Atomic Force Microscopy -- 13: Novel Approach for the Analysis of Interaction Between Sugar Chains and Carbohydrate-Recognizing Molecules Using a Biosensor Based on Surface Plasmon Resonance -- 14: Glycoprotein Detection Using the Light-Addressable Potentiometric Sensor -- Part III: Protein O-Glycosylation.
,
15: Effects of O-Glycosylation on Protein Structure: The Role and Characterization of Heavily O-Glycosylated Mucin Domains -- 16: Predicting Mucin-Type O-GIycosylation Sites -- 17: Experimental Approaches to Studying O-Glycosylation In Vivo -- 18: Identifying Sites of Glycosylation in Proteins -- 19: High Energy Collision-Induced Dissociation of O-Linked Glycopeptides -- 20: Analysis of Permethylated Glycoprotein Oligosaccharide Fractions by Gas Chromatography and Gas Chromatography-Mass Spectrometry -- Part IV: Labeling Oligosaccharides with Chromophores and Fluorophores -- 21: Highly Sensitive Pre-Column Derivatization Procedures for Quantitative Determination of Monosaccharides, Sialic Acids, and Amino Sugar Alcohols of Glycoproteins by Reversed Phase High-Performance Liquid Chromatography -- 22: Glycan Labeling with the Fluorophores 2-Aminobenzamide and Anthranilic Acid -- 23: Capillary Gel Electrophoresis of 8-Aminopyrene-3,6,8-trisulfonate-Labeled Oligosaccharides -- 24: Evaluation of Glycosylpyrazole Derivatives in Carbohydrate Analysis -- Part V: Strategies for Oligosaccharide Separations and Sequencing -- 25: Discovery and Uses of Novel Glycosidases -- 26: Resolution of Oligosaccharides in Capillary Electrophoresis -- 27: A Comparison of Oligosaccharide Profiling Methods -- 28: High-Performance Liquid Chromatographic Mapping of Oligosaccharides Using High pH Anion-Exchange Chromatography: Improvements from Sample Preparation, Reduction, and Fluorometric Detection -- 29: Monosaccharide Analysis of Recombinant Glycoproteins by High pH Anion-Exchange Chromatography/High-Performance Liquid Chromatography Using a Refractive Index Detector -- 30: 2- Aminobenzamide Labeling, Matrix-Assisted Laser Desorption A Sensitive Method for Monitoring Lot-to-Lot Consistency of Recombinant Glycoproteins.
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31: Characterization of Subnanomolar Amounts of N-Glycans by 2-Aminobenzamide Labeling, Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, and Computer-Assisted Sequence Analysis -- 32: Isolation and Characterization of N-Linked Oligosaccharides from Yeast Glycoproteins -- Part VI: Plant Glycobiology -- 33: High Resolution Electrophoretic Analysis of Carbohydrates Using a DNA Sequencer -- 34: Recent Developments in Starch Structure Analysis Using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection -- 35: Red Seaweed Galactans: Methodology for the Structural Determination of Corallinan, a Different Agaroid -- 36: Some New Methods to Study Plant Polyuronic Acids and Their Esters -- 37: Analysis of Red Algal Extracellular Matrix Polysaccharides: Cellulose and Carrageenan -- Index.
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