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  • 1
    Electronic Resource
    Electronic Resource
    Diagnostic potential of chromogenic substrates for rapid detection of bacterial enzymatic activity in health and disease associated periodontal plaques (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 19 (1984), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1984.tb01327.x
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of periodontal therapy on lymphocyte blastogenesis to plaque associated microorganisms (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 18 (1983), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effect of clinical treatment on the in vitro lymphocyte blastogenic response to a panel of dental plaque-associated microorganisms, as well as non-oral antigens, was assessed longitudinally in a group of twenty-two patients during treatment for periodontitis. The patients' blastogenic response to the non-oral antigens remained constant, without, Significant change, throughout the three year study. Following scaling, root planning, and oral hygiene instruction (hygiene phase), an increased blastogenic response to the plaque-associated Stimulants (except B. gingivalis) was observed. Responses measured after Periodontal Surgery and one year following the hygiene phase (first maintenance year) were lower than pretreatment values. Between the first and second yearly maintenance phase evaluations there was a significant increase in the blastogenic response to A. viscosus, B. gingivalis, and F. nucleatum). Thus, periodontal therapy initially resulted in a reduction of plaque antigen-induced lymphocyte blastogenesis. However, the responses increased again with time in the absence of deteriorating clinical parameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1983.tb00340.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of repeated scaling and root planing and/or controlled oral hygiene on the periodontal attachment level and pocket depths in beagle dogs (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 17 (1982), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A study was performed to evaluate the effect of initial and/or repeated scaling with or without controlled oral hygiene on the level of periodontal attachment and pocket depth in beagle dogs. The clinical results of this three-year longitudinal study have been reported previously (Morrison et al. 1979). The purpose of the present report is to present the microbiological results of subgingival plaque samples obtained from selected sites at the conclusion of the study.Eight beagle dogs with moderately developed periodontitis were divided into experimental and control animals. The experimental group received a thorough scaling and root planing at the start of the experiment following which the animals were subjected to daily toothbrushing and rubber cup and pumice prophylaxis every second week for 36 months. Four control dogs were not subjected to any oral hygiene procedures for the entire period of the study. The teeth of two quadrants in each animal of the experimental and control group were scaled and root planed every six months.After three years subgingival plaques from the mesial aspect of the fourth premolar in each of the quadrants of each animal were collected by sterile curettes, processed anaerobi-cally, and cultured in an anaerobic glove box. Significantly lower total viable colony forming units (CFU) as well as significantly lower anaerobe/aerobe ratios were found in the subgingival plaques of the experimental animals. The total CFU of Bacteroides asaccharo-lyticus was 25 times lower, and tie proportion of CFU of this organism was nine times lower in the experimental sites when compared with the control sites. Repeated scaling every six months also lowered the total CFU and the proportion of Bacteroides asaccharo-lyticus in the subgingival plaques of the experimental as well as the control animals. The data suggest that the level of Bacteroides asaccharolyticus as key organisms as well as the anaerobe/aerobe ratio are valuable microbiological parameters in evaluating the efficacy of periodontal therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1982.tb01148.x
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  • 4
    Electronic Resource
    Electronic Resource
    Differences between gingivitis and periodontitis associated microbial flora in the beagle dog (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 17 (1982), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The predominant dental plaque flora was compared between two groups of adult beagle dogs, (1) ten with gingivitis and (2) ten with advanced periodontitis. Plaque from a maxillary third premolar was cultured under strict anaerobic conditions. Specimens comprising the marginal periodontal tissues were taken at the plaque sampling site and analyzed for some histological parameters of periodontal disease. The periodontitis dogs scored significantly higher for crevice depth, length of ulcerated crevice epithelium, area of inflamed connective tissue, and loss of attachment. Supragingival periodontitis plaque had significantly higher anaerobic to aerobic ratio, proportions and CFU of esculin negative streptococci, but lower proportions of Actinomyces viscosus. Subgingival periodontitis plaque had significantly higher anaerobic to aerobic ratio, microscopic counts of spirochetes, total viable CFU, proportions and CFU of esculin negative streptococci and Fusobacterium nucieatum, as well as CPU of Bacteroides asaccharolyticus, Gram negative bacilli and coccobacilli, but significantly lower proportions of A. viscosus and unspeciated actinomycetes. The total viable CFU, proportions and CFU of esculin negative streptococci correlated with all four histological parameters of periodontal disease. The CFU of B. asaccharolyticus, bacilli, and coccobacilli correlated with the length of ulcerated crevice epithelium and loss of attachment, but F. nucleatum only with the area of inflamed connective tissue and loss of attachment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1982.tb01126.x
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  • 5
    Electronic Resource
    Electronic Resource
    The predominant cultivable dental plaque flora of beagle dogs with gingivitis (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 15 (1980), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The predominant dental plaque flora of 15 female beagle dogs (1,3 and 6 year old) with naturally developed gingivitis was studied using a continuous anaerobic culturing technique. Supra- and subgingival plaque samples from the buccal aspect of the upper third premolar were cultured on various growth media and the organisms were partially characterized. The flora in all dogs was composed mostly of anaerobic Gram negative organisms. B. asaccharolyticus (B. melaninogenicus ss. asaccharolyticus) was found in both types of plaques in all animals and decomposed hydrogen peroxide suggesting catalase activity. F. nucleatum was found in higher proportions in the subgingival plaque as compared to B. asaccharolyticus and actinomycetes. Spirochetes were found in 10 of 15 supra- and in 1 of 15 subgingival specimens. S. mutans, S. mitior, S. salivarius, Veillonella, Selenomonas or vibrios could not be detected in any of the plaque samples. A combined data analysis showed, that the total viable CFU and the proportions of Gram positive organisms were significantly higher in the supra- than the subgingival plaque. Although the proportions of Gram negative bacteria were higher in subgingival plaque, the differences between the two types of plaques excluding F. nucleatum were not significant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1980.tb00266.x
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  • 6
    Electronic Resource
    Electronic Resource
    Pulp capping of dental pulp mechanically exposed to oral microflora: a 1–2 year observation of wound healing in the monkey (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 14 (1985), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Four adult Rhesus monkeys provided 120 teeth for buccal Class V cavities. Twenty-nine were non-exposed controls and 91 were exposed for 3 intervals. All 120 teeth were capped with a hard set Ca(OH)2, medicament, restored with amalgam, 57 evaluated after 1 year and 63 after 2 years. Of the 91 exposed pulps, 45 showed complete healing, 25 showed pulpal inflammation varying from acute to chronic, 12 showed severe pulpal breakdown and abscess formation and 9 were necrotic. No difference was observed in the healing response between the 3 exposure times. New hard tissue formed at, or subjacent to, the medicament in 77 of 91 exposed pulps with a tunnel defect frequently present, running from the medicament interlace to the pulp. This study demonstrates that recurring pulp inflammation observed after 1 & 2 year direct pulp capping, is associated with bacterial contamination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1985.tb00479.x
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  • 7
    Electronic Resource
    Electronic Resource
    Bacterial leakage around dental restorations: its effect on the dental pulp (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 11 (1982), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study revealed that the quantity of bacteria filtered from the base of Class V cavity restorations were directly related to the type of medicament used. Of the brands studied: composite, amalgam, silicate, and guttapercha – each produced varying numbers of bacterial colonies, whereas zinc oxide and eugenol cement showed none. Histopathology of the pulps correlated directly to the microbiological data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1982.tb00188.x
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  • 8
    Electronic Resource
    Electronic Resource
    Reduction in proportions of dental plaque streptococci following a 5 day topical kanamycin treatment (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 12 (1977), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A 5 day topical kanamycin treatment of the dental gingival surfaces in institutionalized mentally retarded subjects significantly reduced the plaque mass on the tooth surfaces compared to a placebo treatment. This reduction occurred in the absence of any mechanical hygiene procedures and lasted for 4 or more weeks, which was longer than the kanamycin could have been expected to be present in the mouth. This suggested that the kanamycin had caused an alteration of the plaque flora which should be discernible by quantitative culturing procedures. Plaque was removed and cultured, using an anaerobic serial dilution procedure, before and after kanamycin or placebo treatments. The reduction in plaque weight was associated with reduced levels of streptococci in the plaque. This could be demonstrated by intergroup comparison of the placebo - and kanamycin treated subjects, and by intragroup comparisons of the kanamycin treated subjects prior to treatment and at 4 and 8 weeks after treatment. The streptococci species accounted for 30% of the plaque colony forming units (CFU) prior to kanamycin treatment. At 4 weeks after treatment, the streptococci had decreased to 3% of the CFU and at 8 weeks after treatment, the streptococci had increased slightly to account for 14% of the CFU. The streptococci appeared to be Streptococcus sanguis and Streptococcus mitis. The observations indicate that the topical kanamycin was effective in reducing supragingival plaque because of its ability to decrease the proportions of streptococci in the plaque.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1977.tb00103.x
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  • 9
    Electronic Resource
    Electronic Resource
    Treatment of periodontal infections due to anaerobic bacteria with short-term treatment with metronidazole (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of clinical periodontology 8 (1981), S. 0 
    Details
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract In the present report, five selected periodontal patients were treated for 1 week with metronidazole. Two of the patients had their teeth scaled and root-planed the week they received metronidazole. Prior to treatment, B. asaccharolyticus accounted for 41 % of the cultivable isolates and the spirochetes averaged 29 % of the microscopic count in plaque removed from each of four pockets per patient. The presence of these elevated proportions of periodontopathic bacteria combined with the presence of periodontal pockets and attachment loss suggested that the patients were in a state of an active infectious process involving primarily anaerobic bacteria. If this be the case, then antimicrobial therapy directed against these anaerobes with metronidazole was indicated. The 1-week treatment with metronidazole significantly reduced the proportions of these organisms for up to 6 months after treatment. Coincident with these findings was an improvement in the clinical parameters, especially in those sites that initially had greater than 5 mm pocket or attachment loss. These sites showed a 2 mm or more reduction in pocket depth and an almost 2 mm gain in apparent attachment that was evident 6 months after treatment. The results obtained were in only five patients. However, the magnitude of improvement suggests that antimicrobial therapy directed against anaerobic organisms may be a valuable adjunct to periodontal therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-051X.1981.tb02022.x
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  • 10
    Electronic Resource
    Electronic Resource
    The predominant cultivable dental plaque flora of beagle dogs with periodontitis (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of clinical periodontology 8 (1981), S. 0 
    Details
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract The predominant cultivable dental plaque flora was studied in 10 adult female beagle dogs with advanced periodontitis. Supragingival and subgingival plaque from a maxillary third premolar (P3) was removed and cultured anaerobically on various growth media and all colonies were subcultured and partially characterized. Histopathological specimens of the plaque sampling sites showed significant loss of connective tissue attachment. Spirochetes were found in ail samples. Anaerobic gram-negative organisms were predominant in both types of plaque accounting for about 55 % of the cultivable organisms in the supragingival plaque and almost 75% in the subgingival plaque. Bacteroides asaccharolyticus was the most prominant organism in the supragingival plaque, whereas Fusobacterium nucleatum predominated in the subgingival flora. Streptococcal and actinomycotic species were common in the supragingival plaque, but their proportions, especially those of the actmomycetes, were decreased in the subgingival flora. In many respects the bacterial profile associated with disease resembled that reported in human periodontal disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-051X.1981.tb02023.x
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