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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 270 (1977), S. 76-78 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] PCL and polypropiolactone were prepared by ring opening polymerisation of e-caprolactone9 and p-propiolactone respectively in benzene in a nitrogen atmosphere at 60 C with a diethylzinc-water catalyst system. Poly-p-methylpropiolactone (poly-p-hydroxybutyrate) was made from p-methylpropiolactone by ...
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0983
    Keywords: Key words Glycolytic gene ; Aspergillus oryzae ; Glucose induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We cloned all the glycolytic genes from Aspergillus oryzae and analyzed their transcriptional regulation by the carbon source in the medium. The deduced amino-acid sequences of the glycolytic genes showed high identity (approximately 41–93%) to those from other lower eukaryotes. Genomic Southern hybridization indicated that all the genes existed as a single copy in the genome. Comparison of mRNA levels between mycelia grown on glucose and on pyruvate showed that most of the A. oryzae glycolytic genes were induced by glucose in the medium. The overall expression profiles of the A. oryzae glycolytic genes resembled those of Saccharomyces cerevisiae. The expression of one of the phosphofructokinase genes (pfkB), however, was repressed by glucose while both PFK1 and PFK2 were induced in S. cerevisiae. These findings indicate that further analysis of the transcriptional regulation of the A. oryzae glycolytic genes will be useful for investigating the evolutionary change of transcription regulation in lower eukaryotes and to construct promoters for industrial applications.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 20 (1984), S. 378-383 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A phthalate ester hydrolyzing enzyme has been purified from the culture broth of Nocardia erythropolis, a Gram-positive bacterium capable of degrading phthalate esters rapidly. The purified enzyme appeared homogeneous on polyacrylamide gel disc-electrophoresis, and its molecular weight was estimated to be about 15,000. The optimal pH and temperature were pH 8.6 and 42°C, respectively. The enzyme was stable in a pH range from 7.0 to 8.0 and below 30°C. The enzyme activity was stimulated by Ca2+ and taurocholate, but inhibited by several metals such as Hg2+. Most of the phthalate esters tested were hydrolyzed to phthalate and alcohols regardless of the type of side-chain. In addition, the enzyme rapidly hydrolyzed olive oil and tributyrin. This enzyme from N. erythropolis may be a novel type of lipase with broad substrate specificity.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biodegradation 7 (1996), S. 41-48 
    ISSN: 1572-9729
    Keywords: natural rubber ; Nocardia sp. ; colonization ; degradation rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The growth of a Nocardia sp. occurs essentially on the insoluble rubber substrate and the cells are tightly bound to the rubber in the initial stage of the growth in spite of vigorous stirring of the cultures. The colonization of rubber pieces was followed by staining with Schiff reagent, and it was revealed that not only the thickness of rubber pieces, but also their length and width greatly influenced microbial colonization and degradation of natural rubber products. Among rubber pieces of various shapes, long strips were most rapidly covered by many microbial colonies and experienced the highest rate of rubber degradation. The rate of degradation (expressed by % weight loss) of the long strips of rubber was a linear function of surface area per unit weight of rubber. Thin and wide films of rubber were also rapidly colonized and degraded, while the colonization and degradation of short and narrow pieces were substantially slower and less extensive.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 26 (1981), S. 441-448 
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Copolyesters (CPEs) prepared by the transesterification reaction between aromatic and aliphatic polyesters were hydrolyzed by Rhizopus delemar lipase. The susceptibility of CPEs to hydrolysis by this lipase dropped off rapidly during the initial stage of the transesterification reaction and increased gradually as the reaction proceeded. The susceptibility to hydrolysis decreased with increase in aromatic polyester content. It was concluded that the rigidity of the aromatic ring in the CPE chains strongly influenced their susceptibility to hydrolysis by this lipase.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Publication Date: 2017-06-02
    Description: Aims To compare the oncogenic mutation status among mucinous cystic neoplasms (MCNs) of different histological grades and between liver and pancreatic MCNs. Methods and Results KRAS , GNAS , RNF43 and PIK3CA were sequenced in 25 surgical cases of hepatopancreatic MCNs. Molecular features were correlated with clinicopathological and immunohistochemical findings. KRAS mutations were identified in 5 cases (20%), while GNAS , RNF43 and PIK3CA were the wild-type in all cases. KRAS mutations were uncommon in cases of low-grade dysplasia (1/20, 5%), while KRAS was mutated in all cases of higher grades, except for one liver MCN with intermediate-grade dysplasia (4/5, 80%; p=0.002). This genetic alteration was slightly more frequent in the pancreas than in the liver (4/17 [24%] vs. 1/8 [13%], p=NS). KRAS -mutated MCNs more commonly showed a multilocular cystic appearance (p=0.040) and the expression of MUC1 (p=0.040), MUC2 (p=0.016), and MUC5AC (p=0.015) than KRAS -wild-type tumours. In cases of KRAS -mutated MCNs with intermediate- or high-grade dysplasia, identical mutations were also detected in areas of adjacent low-grade dysplasia. Conclusions KRAS mutations appear to be a major driver genetic alteration in both liver and pancreatic MCNs. Since identical KRAS mutations were present in low- and higher-grade areas in individual cases, KRAS mutations occurring in low-grade MCNs may lead to tumour progression. Thus, preoperative KRAS testing may contribute to estimations of malignant potential. The lower incidence of KRAS mutations in liver MCNs may also explain why the risk of malignant transformation in liver MCNs is lower than that in pancreatic MCNs. This article is protected by copyright. All rights reserved.
    Print ISSN: 0309-0167
    Electronic ISSN: 1365-2559
    Topics: Medicine
    Published by Wiley-Blackwell
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