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  • 1
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Eight new strains of deep-sea hyperthermophilic sulfur reducers were isolated from hydrothermal vent fields at 9°50′N East Pacific Rise (EPR) and at the Cleft and CoAxial segments along the Juan de Fuca Ridge (JdFR). 16S rRNA gene sequence analysis showed that each strain belongs to the genus Thermococcus. Restriction fragment length polymorphism patterns of the 16S/23S rRNA intergenic spacer region revealed that these isolates fell into three groups: those from the EPR, those from fluid and rock sources on the JdFR, and those isolated from Paralvinella spp. polychaete vent worms from the JdFR. The optimum-temperature specific growth rates and the temperature ranges for growth were significantly higher and broader for those strains isolated from worms relative to those isolated from low-temperature diffuse hydrothermal fluids. Furthermore, the worm-derived isolates generally produced a larger array of proteases and amylases based on zymogram analyses. The zymogram patterns also changed with growth temperature suggesting that these organisms alter their lytic protein suites in response to changes in temperature. This study suggests that there is significant phenotypic diversity in Thermococcus that is not apparent from their highly conserved 16S rRNA nucleotide sequences.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 25 (1998), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Thermophilic and hyperthermophilic microorganisms were cultured from 18°C diffuse hydrothermal fluids at the CoAxial segment deep-sea hydrothermal vent site 3 months after an eruption resulting from an intrusion of magma into shallow crust. The abundances of these organisms decreased over a 3-year period as the shallow magma cooled. The presence of these organisms at the site suggests that these organisms grew in response to nutrient input from hydrothermal fluid circulation and then were flushed to the surface following the eruption. Thermophiles and hyperthermophiles were also found in low-temperature (3–30°C) fluids at three other chronic, highly active deep-sea vent sites. The origin of these organisms is not known but may include the overlying seawater or a shallow to deep subseafloor habitat.
    Type of Medium: Electronic Resource
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  • 3
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    PANGAEA
    In:  Supplement to: Summit, Melanie; Peacock, Aaron D; Ringelberg, David; White, David C; Baross, John A (2000): Phospholipid fatty acid-derived microbial biomass and community dynamics in hot, hydrothermally influenced sediments from Middle Valley, Juan De Fuca Ridge. In: Zierenberg, RA; Fouquet, Y; Miller, DJ; Normark, WR (eds.) Proceedings of the Ocean Drilling Program, Scientific Results, College Station, TX (Ocean Drilling Program), 169, 1-19, https://doi.org/10.2973/odp.proc.sr.169.117.2000
    Publication Date: 2024-01-09
    Description: Phospholipid fatty acids were measured in samples of 60°-130°C sediment taken from three holes at Site 1036 (Ocean Drilling Program Leg 169) to determine microbial community structure and possible community replacement at high temperatures. Five of six samples had similar concentrations of phospholipid fatty acids (2-6 pmol/g dry weight of sediment), and biomass estimates from these measurements compare favorably with direct microscopic counts, lending support to previous microscopic measures of deep sedimentary biomass. Very long-chain phospholipid fatty acids (21 to 30 carbons) were detected in the sediment and were up to half the total phospholipid fatty acid measured; they appear to increase in abundance with temperature, but their significance is not known. Community composition from lipid analysis showed that samples contained standard eubacterial membrane lipids but no detectable archaeal lipids, though archaea would be expected to dominate the samples at high temperatures. Cluster analysis of Middle Valley phospholipid fatty acid compositions shows that lipids in Middle Valley sediment samples are similar to each other at all temperatures, with the exception of very long-chain fatty acids. The data neither support nor deny a shift to a high-temperature microbial community in hot cores, so at the present time we cannot draw conclusions about whether the microbes observed in these hot sediments are active.
    Keywords: Ocean Drilling Program; ODP
    Type: Dataset
    Format: application/zip, 2 datasets
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  • 4
    Publication Date: 2024-01-09
    Keywords: 169-1036A; 169-1036B; 169-1036C; Ammonium; Bacteria, abundance; Calcium; Chlorine; Dead Dog vent field, North Pacific Ocean; DEPTH, sediment/rock; DRILL; Drilling/drill rig; DSDP/ODP/IODP sample designation; Escanaba Trough, North Pacific Ocean; Event label; Fatty acids, very long chain; Joides Resolution; Leg169; Magnesium; Ocean Drilling Program; ODP; pH; Phospholipid fatty acids; Sample code/label; Sulfate; Temperature, in rock/sediment
    Type: Dataset
    Format: text/tab-separated-values, 72 data points
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  • 5
    Publication Date: 2024-01-09
    Keywords: 12-methyl-Tetradecanoic acid of total fatty acids (IUPAC: 12-methyltetradecanoic acid); 14-methyl-Hexadecanoic acid of total fatty acids (IUPAC: 14-methylhexadecanoic acid); 169-1036A; 169-1036B; 169-1036C; cis-11-Octadecenoic acid of total fatty acids (IUPAC: Octadec-11-enoic acid); cis-9-Hexadecenoic acid of total fatty acids (IUPAC: (9Z)-hexadec-9-enoic acid); cis-9-Octadecenoic acid of total fatty acids (IUPAC: Octadec-9-enoic acid); cyclo-Nonadecanoic acid of total fatty acids (IUPAC: Methyleneoctadecanoic acid); Cyclopropane fatty acid of total fatty acids (IUPAC: Methylenehexadecanoic acid); Dead Dog vent field, North Pacific Ocean; DEPTH, sediment/rock; Docosanoic acid of total fatty acids; DRILL; Drilling/drill rig; DSDP/ODP/IODP sample designation; Escanaba Trough, North Pacific Ocean; Event label; Gas chromatography - Mass spectrometry (GC-MS); Heneicosanoic acid of total fatty acids; Heptacosanoic acid of total fatty acids; Hexacosanoic acid of total fatty acids; Hexadecanoic acid of total fatty acids; iso-Heptadecanoic acid of total fatty acids (IUPAC: 15-methylhexadecanoic acid); iso-Hexadecanoic acid of total fatty acids (IUPAC: 14-methylpentadecanoic acid); iso-Pentadecanoic acid of total fatty acids (IUPAC: 13-methyltetradecanoic acid); Joides Resolution; Leg169; Nonacosanoic acid of total fatty acids; Ocean Drilling Program; Octacosanoic acid of total fatty acids; Octadecanoic acid of total fatty acids; ODP; Pentacosanoic acid of total fatty acids; Sample code/label; Sum; Tetracosanoic acid of total fatty acids; Triacontanoic acid of total fatty acids; Tricosanoic acid of total fatty acids
    Type: Dataset
    Format: text/tab-separated-values, 140 data points
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