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  • 1
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    Activation of an Innate Immune Receptor, Nod1, Accelerates Atherogenesis in Apoe-/- Mice [INNATE IMMUNITY AND INFLAMMATION] (2015)
    The American Association of Immunologists (AAI)
    Details
    Publication Date: 2015-01-03
    Description: Atherosclerosis is essentially a vascular inflammatory process in the presence of an excess amount of lipid. We have recently reported that oral administration of a nucleotide-binding oligomerization domain (Nod)-1 ligand, FK565, induced vascular inflammation in vivo. No studies, however, have proven the association between Nod1 and atherosclerosis in vivo. To investigate a potential role of NOD1 in atherogenesis, we orally administered FK565 to apolipoprotein E knockout ( Apoe –/– ) mice for 4 wk intermittently and performed quantification of atherosclerotic lesions in aortic roots and aortas, immunohistochemical analyses, and microarray-based gene expression profiling of aortic roots. FK565 administration accelerated the development of atherosclerosis in Apoe –/– mice, and the effect was dependent on Nod1 in non–bone marrow origin cells by bone marrow transplantation experiments. Immunohistochemical studies revealed the increases in the accumulation of macrophages and CD3 T cells within the plaques in aortic roots. Gene expression analyses of aortic roots demonstrated a marked upregulation of the Ccl5 gene during early stage of atherogenesis, and the treatment with Ccl5 antagonist significantly inhibited the acceleration of atherosclerosis in FK565-administered Apoe –/– mice. Additionally, as compared with Apoe –/– mice, Apoe and Nod1 double-knockout mice showed reduced development of atherosclerotic lesions from the early stage as well as their delayed progression and a significant reduction in Ccl5 mRNA levels at 9 wk of age. Data in the present study show that the Nod1 signaling pathway in non–bone marrow-derived cells contributes to the development of atherosclerosis.
    Print ISSN: 0022-1767
    Electronic ISSN: 1550-6606
    Topics: Medicine
    Published by The American Association of Immunologists (AAI)
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  • 2
    Electronic Resource
    Electronic Resource
    Efficacy and Limitations of Cyclophosphamide-induced Tolerance against αGal Antigen (2005)
    Oxford, UK; Malden, USA : Blackwell Science Ltd
    Scandinavian journal of immunology 62 (2005), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In the present study, we have elucidated the efficacy of two cyclophosphamide (CP)-induced tolerance protocols for the induction of B-cell tolerance against Galα1-3Galβ1-4GlcNAc (αGal) antigens. α1,3-galactosyltransferase-deficient (GalT–/–; H-2b/d) mice received with 1 × 108 AKR (αGal+/+ H-2k) spleen cells (SC) followed by 200 mg/kg CP, or alternatively followed by 200 mg/kg CP, 30 mg/kg Busulfan (BU) and 1 × 108 T-cell-depleted AKR bone marrow cells (BMC). The generation of both anti-αGal and anti-donor antibodies were completely suppressed, but normal antibody production against third party antigens was observed after BALB/c skin grafting in both groups of GalT–/– mice. In GalT–/– mice, treated with SC and CP, mixed chimerism was not observed. Cellular rejection was observed in grafted donor AKR hearts with an absence of humoral rejection, whereas humoral rejection was observed in untreated GalT–/– mice. On the other hand, long-term mixed chimerism and permanent acceptance of donor AKR skin graft and heart graft were achieved in GalT–/– mice treated with SC, CP, BU and BMC. These results demonstrate the efficacy of classical drug-induced tolerance in the induction of B-cell tolerance against αGal antigens. However, induction of stable mixed chimerism was required for the suppression of cellular rejection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.2005.01644.x
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  • 3
    Electronic Resource
    Electronic Resource
    Purification and characterization of human kidney plasminogen activator dissimilar to urokinase
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 717 (1982), S. 327-336 
    Details
    ISSN: 0304-4165
    Keywords: (Human kidney) ; Plasminogen activator ; Urokinase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-4165(82)90187-8
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation of a major apolipoprotein of canine and murine pulmonary surfactant Biochemical and immunochemical characteristics
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 665 (1981), S. 442-453 
    Details
    ISSN: 0005-2760
    Keywords: (Canine lung, Murine lung) ; Apolipoprotein ; Detergent ; Immunoassay ; Pulmonary surfactant
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2760(81)90257-5
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  • 5
    Electronic Resource
    Electronic Resource
    Role of calcium ions in the structure and function of pulmonary surfactant
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 793 (1984), S. 18-27 
    Details
    ISSN: 0005-2760
    Keywords: (Dog) ; Ca^2^+, Phospholipid structure ; Lung surfactant ; Tubular myelin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2760(84)90048-1
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  • 6
    Electronic Resource
    Electronic Resource
    Secretion of surfactant by primary cultures of alveolar type II cells isolated from rats
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism 713 (1982), S. 118-127 
    Details
    ISSN: 0005-2760
    Keywords: (Rat) ; Alveolar type II cell ; Lung surfactant ; Phosphatidylcholine ; Phosphatidylglycerol ; Phospholipid secretion
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2760(82)90174-6
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  • 7
    Electronic Resource
    Electronic Resource
    Tumor necrosis factor alpha-induced alteration of glycosaminoglycans in cultured vascular smooth-muscle cells
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Cell Research 1176 (1993), S. 20-26 
    Details
    ISSN: 0167-4889
    Keywords: (Bovine vascular smooth-muscle cell) ; Cytokine ; Glycosaminoglycan ; Tumor necrosis factor α
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4889(93)90172-L
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  • 8
    Electronic Resource
    Electronic Resource
    Conditioned media of carcinoma cells cultured in hypoxic microenvironment stimulate angiogenesis in vitro; relationship to basic fibroblast growth factor (1995)
    Springer
    Virchows Archiv 425 (1995), S. 561-568 
    Details
    ISSN: 1432-2307
    Keywords: Angiogenesis ; Fibroblast growth factor basic ; Endothelial cells ; Hypoxia ; Neoplasms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Conditioned media (CM) harvested from human pulmonary squamous cell carcinoma (QG56), pulmonary small cell carcinoma (QG90) and gastric adenocarcinoma (MKN28) cultivated under hypoxic conditions (3% oxygen), enhanced the angiogenic activity in vitro more than those obtained under normoxic cultivation (20% oxygen). The total length of the tube structures formed by bovine capillary endothelial cells (BCEs) in the CM cultured at 3% oxygen was about 1.5 (QG56 and MKN28) or 1.9 (QG90) times longer than that at 20% oxygen. Tube formation was diminished by the preincubation of CM with anti-basic fibroblast growth factor (bFGF) IgG. After performing the fractionations of the CM and the crude extracts of cell lysates cultured using a heparin-Sepharose column, the mitogenic activity in the CM from all cancer cells at 3% oxygen was about twice that of CM at 20% oxygen, while it decreased in the cell lysates at 3% oxygen to about 40% of those at 20% oxygen. This mitogenic activity of BCEs in the CM from all cancer cells was almost totally suppressed by anti-bFGF IgG, but not with anti-vascular endothelial growth factor IgG. Hypoxia is an important factor in tumour angiogenesis by bFGF or bFGF-like molecule(s) derived from tumour cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00199343
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  • 9
    Electronic Resource
    Electronic Resource
    Hypoxia-induced expression of vascular endothelial growth factor by retinal glial cells promotes in vitro angiogenesis (1995)
    Springer
    Virchows Archiv 426 (1995), S. 479-486 
    Details
    ISSN: 1432-2307
    Keywords: Vascular endothelial growth factor ; Retinal glial cells ; Hypoxia ; Angiogenesis ; Retinal capillary endothelial cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To determine whether retinal glial cells (RGCs) participate in the paracrine regulation of retinal neovascularization, we investigated whether cultured RGCs synthesize and release vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) under normoxic or hypoxic conditions. Northern blot analysis demonstrated that cultured RGCs transcribed both VEGF mRNA with two molecular bands approximately 3.9 and 4.3 kilobases (kb), and bFGF mRNA with approximately 3.7 and 6.0 kb. The expression of VEGF mRNA was greatly enhanced by hypoxic cultivation (2% oxygen) when compared with normoxic cultivation (20% oxygen), while the expression of bFGF mRNA by RGCs was not significantly affected by hypoxia. The effects of RGCs-conditioned media (CM) on tritiated-thymidine incorporation and in vitro angiogenesis by retinal capillary endothelial cells (RECs) in producing the formation of capillary-like tubes in type I collagen gels, were evident in the observation that RGCs-CM harvested after hypoxic cultivation significantly enhanced tritiated-thymidine incorporation (1.9 times, P〈0.01) and in vitro angiogenesis (2.4 times, P〈0.01) compared with the normoxic RGCs-CM. These enhancing effects of RGCs-CM at hypoxia were suppressed by anti-VEGF neutralizing antibody. Furthermore, RECs were shown to express mRNA encoding the VEGF receptor flt-1 by northern blot analysis. These results suggest that VEGF expressed by RGCs under hypoxic conditions plays an integral role in the initiation and progression of retinal neovascularization in a paracrine manner.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193171
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  • 10
    Electronic Resource
    Electronic Resource
    A monoclonal antibody that defines basal cells of stratified epithelia in various human and rabbit tissues (1989)
    Springer
    Histochemistry and cell biology 92 (1989), S. 319-324 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monoclonal antibodies were produced against monkey lung lavage fluid by using a mouuse hybridoma technique. One monoclonal antibody, KP8D4, specifically reacted with basal cells in human bronchial epithelia by immunohistological staining of acetone-fixed, frozen sections and it recognized a protein with an apparent molecular weight of 84000, as determined by gel immunoblotting. The distribution of this protein was immunohistochemically examined in various human tissues (lung, tongue, esophagus, stomach, intestine, liver, pancreas, salivary gland, spleen, thymus, heart, aorta, vena cava, prostate, breast, kidney, urinary bladder, thyroid, brain, skin, striated muscle) and various tissues of rats, rabbits and pigs. The results showed a specific affinity of KP8D4 to basal cells of stratified epithelia in the various human and rabbit tissues. This antibody may be a useful tool for studies of normal development and diverse pathological disorders.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500547
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