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Seawater carbonate chemistry and combined mechanistic effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101, 2010 (2010)

Levitan, Orly ; Kranz, Sven A ; Spungin, D ; [et al.]

PANGAEA

In: Supplement to: Levitan, Orly; Kranz, Sven A; Spungin, D; Prasil, O; Rost, Björn; Beran-Frank, Ilana (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: A mechanistic view. Plant Physiology, 154, 346-356, https://doi.org/10.1104/pp.110.159285

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Publication Date: 2024-03-15

Description: The marine diazotrophic cyanobacterium Trichodesmium responds to elevated atmospheric CO2 partial pressure (pCO2) with higher N2 fixation and growth rates. To unveil the underlying mechanisms, we examined the combined influence of pCO2(150 and 900 µatm) and light (50 and 200 µmol photons m-2 s-1) on TrichodesmiumIMS101. We expand on a complementary study that demonstrated that while elevated pCO2 enhanced N2 fixation and growth, oxygen evolution and carbon fixation increased mainly as a response to high light. Here, we investigated changes in the photosynthetic fluorescence parameters of photosystem II, in ratios of the photosynthetic units (photosystem I:photosystem II), and in the pool sizes of key proteins involved in the fixation of carbon and nitrogen as well as their subsequent assimilation. We show that the combined elevation in pCO2 and light controlled the operation of the CO2-concentrating mechanism and enhanced protein activity without increasing their pool size. Moreover, elevated pCO2 and high light decreased the amounts of several key proteins (NifH, PsbA, and PsaC), while amounts of AtpB and RbcL did not significantly change. Reduced investment in protein biosynthesis, without notably changing photosynthetic fluxes, could free up energy that can be reallocated to increase N2 fixation and growth at elevated pCO2 and light. We suggest that changes in the redox state of the photosynthetic electron transportchain and posttranslational regulation of key proteins mediate the high flexibility in resources and energy allocation in Trichodesmium. This strategy should enableTrichodesmium to flourish in future surface oceans characterized by elevated pCO2, higher temperatures, and high light.

Keywords: Alkalinity, Gran titration (Gran, 1950); Alkalinity, total; Aragonite saturation state; Bacteria; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; CF1 subunit of ATP synthase protein; CF1 subunit of ATP synthase protein, standard deviation; Comment; Cyanobacteria; Effective absorbance cross-section of photosystem II; Effective absorbance cross-section of photosystem II, standard deviation; Electron transport rate of photosystem II, per cell; Electron transport rate of photosystem II, standard deviation; EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experimental treatment; Fluorescence, intrinsic; Fluorescence, intrinsic, standard deviation; Fluorescence, maximum; Fluorescence, maximum, standard deviation; Fluorescence, variable; Fluorescence, variable, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); GlnA subunit of Gln synthetase; GlnA subunit of Gln synthetase, standard deviation; Iron protein of nitrogenase; Iron protein of nitrogenase, standard deviation; Laboratory experiment; Laboratory strains; Light; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Open photosystem II reaction centers; Open photosystem II reaction centers, standard deviation; Other metabolic rates; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Photosynthetic protein, PsbA, standard deviation; Photosynthetic protein, PsbC; Photosynthetic protein, PsbC, standard deviation; Photosynthetic protein PsbA; Photosynthetic protein Rubisco; Photosynthetic protein Rubisco, standard deviation; Phytoplankton; Primary production/Photosynthesis; Radiation, photosynthetically active; Salinity; see reference(s); Single species; Temperature, water; Time in hours; Trichodesmium sp.

Type: Dataset

Format: text/tab-separated-values, 608 data points

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Trichodesmium's strategies to alleviate phosphorus limitation in the future acidified oceans (2014)

Spungin, D ; Berman-Frank, I ; Levitan, Orly

PANGAEA

In: Supplement to: Spungin, D; Berman-Frank, I; Levitan, Orly (2014): Trichodesmium's strategies to alleviate phosphorus limitation in the future acidified oceans. Environmental Microbiology, 16(6), 1935-1947, https://doi.org/10.1111/1462-2920.12424

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Publication Date: 2024-05-27

Description: Global warming may exacerbate inorganic nutrient limitation, including phosphorus (P), in the surface-waters of tropical oceans that are home to extensive blooms of the marine diazotrophic cyanobacterium, Trichodesmium. We examined the combined effects of P limitation and pCO2, forecast under ocean acidification scenarios, on Trichodesmium erythraeum IMS101 cultures. We measured nitrogen acquisition, glutamine synthetase activity, C uptake rates, intracellular Adenosine Triphosphate (ATP) concentration and the pool sizes of related key proteins. Here, we present data supporting the idea that cellular energy re-allocation enables the higher growth and N2 fixation rates detected in Trichodesmium cultured under high pCO2. This is reflected in altered protein abundance and metabolic pools. Also modified are particulate organic carbon and nitrogen production rates, enzymatic activities, and cellular ATP concentrations. We suggest that adjusting these cellular pathways to changing environmental conditions enables Trichodesmium to compensate for low P availability and to thrive in acidified oceans. Moreover, elevated pCO2 could provide Trichodesmium with a competitive dominance that would extend its niche, particularly in P-limited regions of the tropical and subtropical oceans.

Keywords: Adenosine 5-Triphosphate, per cell; Adenosine 5-Triphosphate, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bacteria; Bicarbonate ion; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, particulate, production per cell; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Carbon/Phosphorus ratio; Carbon/Phosphorus ratio, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon dioxide, standard deviation; Carbon uptake rate, standard deviation; Carbon uptake rate per cell; CF1 subunit of ATP synthase protein; CF1 subunit of ATP synthase protein, standard deviation; Chlorophyll a, standard deviation; Chlorophyll a per cell; Cyanobacteria; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); GlnA subunit of Gln synthetase; GlnA subunit of Gln synthetase, standard deviation; Glutamine synthetase biosynthetic activity, standard deviation; Glutamine synthetase biosynthetic activity per cell; Glutamine synthetase transferase/biosynthetic activity ratio; Glutamine synthetase transferase/biosynthetic activity ratio, standard deviation; Growth rate; Growth rate, standard deviation; Incubation duration; Iron protein of nitrogenase; Iron protein of nitrogenase, standard deviation; Laboratory experiment; Laboratory strains; Length; Length, standard deviation; Macro-nutrients; Nitrogen/Phosphorus ratio; Nitrogen/Phosphorus ratio, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate organic carbon, per cell; Particulate organic carbon, production, standard deviation; Particulate organic carbon content per cell, standard deviation; Particulate organic nitrogen, standard deviation; Particulate organic nitrogen per cell; Particulate organic nitrogen production, standard deviation; Particulate organic phosphorus, standard deviation; Particulate organic phosphorus per cell; Pelagos; pH; pH, standard deviation; Phosphate; Photosynthetic protein, PsbA, standard deviation; Photosynthetic protein, PsbC; Photosynthetic protein, PsbC, standard deviation; Photosynthetic protein PsbA; Photosynthetic protein Rubisco; Photosynthetic protein Rubisco, standard deviation; Phytoplankton; Primary production/Photosynthesis; Production of particulate organic nitrogen; Salinity; Single species; Species; Temperature, water; Trichodesmium erythraeum

Type: Dataset

Format: text/tab-separated-values, 1003 data points

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