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  • 1
    Electronic Resource
    Electronic Resource
    Simple molecular method to distinguish the identity of Calanus species (Copepoda: Calanoida) at any developmental stage (1999)
    Springer
    Marine biology 133 (1999), S. 91-96 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diagnostic morphological characteristics of copepods of the genus Calanus are restricted largely to minor variations in secondary sex characteristics. This presents a persistent problem in the identification of individuals to species level, especially for immature stages. We have developed a simple molecular technique to distinguish between the North Atlantic Calanus species (C. helgolandicus, C. finmarchicus, C. glacialis and C. hyperboreus) at any life stage. Using the polymerase chain-reaction (PCR), the mitochodrial large subunit (16S) ribosomal RNA (rRNA) gene was amplified from individual copepods preserved in ethanol. Subsequent digestion of the amplified products with the restriction enzymes DdeI and VspI, followed by electrophoretic separation in 2% agarose (Metaphor, FMC Ltd), produced a characteristic pattern for each species. The versatility of the method is demonstrated by the unambiguous identification to species of any life stage, from egg to adult, and of individual body parts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050446
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Stable production of human gastric lipase by chromosomal integration in the fission yeast Schizosaccharomyces pombe (1998)
    Springer
    Applied microbiology and biotechnology 49 (1998), S. 45-50 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Strains of the fission yeast Schizosaccharomyces pombe have been constructed containing single or multiple chromosomally integrated copies of an expression cassette for production of human gastric lipase. Integrant strains of S. pombe secrete active lipase and are stable for lipase production over a minimum of 50 generations in non-selective media. Lipase activity levels for integrant strains containing up to three tandem copies of the expression cassette are strongly correlated with copy number of the cassette in both complete and minimal media. Lipase activity is higher in complete medium than in minimal medium. Strains carrying three chromosomally integrated expression cassette copies can be grown without selection in complete medium and are capable of significantly higher lipase activities than strains containing the expression cassette on a multicopy plasmid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051135
    Location Call Number Limitation Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
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    Integrating conventional microscopy and molecular analysis to analyse the abundance and distribution of four Calanus congeners in the North Atlantic (2005)
    Oxford Univ. Press
    In:  Journal of Plankton Research, 28 (2). pp. 221-238.
    Details
    Publication Date: 2020-07-20
    Description: Analysis of the demographic structure of Calanus species in the North Atlantic presents particular difficulties due to the overlapping spatial distributions of four main congeneric species (Calanus finmarchicus, Calanus helgolandicus, Calanus glacialis and Calanus hyperboreus). These species have similar morphologies, making microscopic discrimination only possible between some of the species at late copepodite or adult stages. However, molecular techniques now offer the possibility of screening significant numbers of specimens and unambiguously identifying them to species, regardless of developmental stage. Unfortunately, the processing rate of specimens by molecular methods is still too low to offer a realistic alternative to microscopy for analysis of samples from large field surveys. Here, we outline and test an approach involving the use of molecular methodology in conjunction with conventional microscopy to assess the species assignment of developmental stage abundances of Calanus congeners. Our study has highlighted many important methodological issues. First, it cannot be assumed that the species composition is homogeneous across the development stages; applying proportional species composition of adults to morphologically undistinguishable earlier development stages can result in error. The second important conclusion is that prosome length may be a highly unreliable discriminator of C. finmarchicus and C. glacialis
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1093/plankt/fbi115
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 4
    facet.materialart.
    Unknown
    Integrating conventional microscopy and molecular analysis to analyse the abundance and distribution of four Calanus congeners in the North Atlantic (2005)
    Oxford Univ. Press
    In:  Journal of Plankton Research, 28 (2). pp. 221-238.
    Details
    Publication Date: 2020-07-20
    Description: Analysis of the demographic structure of Calanus species in the North Atlantic presents particular difficulties due to the overlapping spatial distributions of four main congeneric species (Calanus finmarchicus, Calanus helgolandicus, Calanus glacialis and Calanus hyperboreus). These species have similar morphologies, making microscopic discrimination only possible between some of the species at late copepodite or adult stages. However, molecular techniques now offer the possibility of screening significant numbers of specimens and unambiguously identifying them to species, regardless of developmental stage. Unfortunately, the processing rate of specimens by molecular methods is still too low to offer a realistic alternative to microscopy for analysis of samples from large field surveys. Here, we outline and test an approach involving the use of molecular methodology in conjunction with conventional microscopy to assess the species assignment of developmental stage abundances of Calanus congeners. Our study has highlighted many important methodological issues. First, it cannot be assumed that the species composition is homogeneous across the development stages; applying proportional species composition of adults to morphologically undistinguishable earlier development stages can result in error. The second important conclusion is that prosome length may be a highly unreliable discriminator of C. finmarchicus and C. glacialis.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.1093/plankt/fbi115
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    OceanRep: Article in a Scientific Journal - peer-reviewed
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