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  • 1
    facet.materialart.
    Unknown
    Molecular and morphological characterization of Glossina sp. and identification of associated Trypanosoma sp. in Nigeria including analysis for sialidase activity (2020)
    PANGAEA
    Details
    Publication Date: 2023-10-07
    Description: These data sets include morphological and molecular data generated from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks (Kainji Lake National Park, Old Oyo National Park, Cross River National Park, Gashaka Gumti National Park), one Game Reserve (Yankari Game Reserve) and two tsetse invested areas (Ijah Gwari, Tuongo). Each tsetse was identified morphologically and dissected in the field, all tissues preserved and used for further analysis. Selected tsetse wings were mounted and photographed to be used for morphometric analysis. The whole gut of each tsetse was homogenized in the field and partially preserved for DNA analysis and used to investigate for sialidase activity with a fluorescent substrate and a natural substrate. Analysis for sialidase activity was done by fluorescent measurement and reverse phase HPLC of processed gut samples. DNA was purified from preserved homogenized gut and used for phylogenetic analysis of tsetse by amplification of Cytochrome Oxidase C SU1 and Internal Transcribed Spacer-1 (ITS1). Presence of trypanosomes was investigated in gut and crudely extracted DNA from proboscis by amplification of ITS1. Identification of the respective trypanosome species was done based on lengths polymorphism of the ITS1 region as well as sequencing of selected ITS1 amplicons and additional glycosomal Glyceraldehyde-3-phosohate dehydrogenase.
    Type: Dataset
    Format: application/zip, 6 datasets
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 2
    facet.materialart.
    Unknown
    Tryapanosoma prevalence in tsetse proboscis (Glossina sp.) collected in protected and tsetse invested areas in Nigeria (2020)
    PANGAEA
    Details
    Publication Date: 2023-10-07
    Description: This data set includes the results of nested PCR-analysis of tsetse (Glossina sp.) proboscis samples with primers described by Adams et.al. (2006) and Hamilton et al (2004) from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field. DNA was crudely extracted from the proboscis by Proteinase K digest. Presence of trypanosomes was investigated by amplification of Internal Transcribed Spacer-1 (ITS1). Identification of the respective trypanosome species was done based on lengths polymorphism of the ITS1 region by estimating the approximate size of the DNA amplicon based on electrophoretic separation in agarose gels, as well as sequencing of selected ITS1 amplicons and additional glycosomal Glyceraldehyde-3-phosohate dehydrogenase.
    Keywords: Animal African Trypanosomiasis in humans: An emerging zoonosis?; The role of African trypanosomal trans-sialidases in the transmission of Trypanosoma congolense between the fly vector and life stock host suffering Nagana disease; TRYPTS; TRYPZOO
    Type: Dataset
    Format: application/zip, 4.8 kBytes
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 3
    facet.materialart.
    Unknown
    Tsetse wing pictures (Glossina sp.) from tsetse collected in protected and tsetse invested areas in Nigeria (2020)
    PANGAEA
    Details
    Publication Date: 2023-10-07
    Description: This data set includes images of tsetse wings generated from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field. Selected tsetse wings were mounted in Faures' Solution and photographed to be used for morphometric analysis of wing size and relative vain distances. Species included are G. morsitans submorsitans, G. tachinoides, G. palpalis palpalis and some unidentified Glossina species.
    Keywords: 234-CRNP-T003b; 234-CRNP-T004b; 234-CRNP-T005b; 234-CRNP-T012b; 234-CRNP-T014b; 234-CRNP-T015b; 234-CRNP-T016b; 234-CRNP-T017b; 234-KNP-T001b; 234-KNP-T002b; 234-KNP-T004b; 234-ONP-T002b; 234-T-T007b; 234-T-T008b; 234-T-T014b; 234-YGR-T001b; 234-YGR-T002c; 234-YGR-T004c; 234-YGR-T005c; 234-YGR-T006c; 234-YGR-T008c; 234-YGR-T009c; 234-YGR-T010c; 234-YGR-T011c; 234-YGR-T012c; 234-YGR-T013c; 234-YGR-T014c; 234-YGR-T015c; 234-YGR-T016c; 234-YGR-T018c; Animal African Trypanosomiasis in humans: An emerging zoonosis?; BICON; Collector; Cross River National Park, Nigeria, Africa; Event label; Insecttrap, biconical; Kainji Lake National Park, Nigeria, Africa; Old Oyo National Park, Nigeria, Africa; Sample ID; The role of African trypanosomal trans-sialidases in the transmission of Trypanosoma congolense between the fly vector and life stock host suffering Nagana disease; Toungo Area, Nigeria, Africa; TRYPTS; TRYPZOO; Uniform resource locator/link to image; Yankari Game Reserve, Nigeria, Africa
    Type: Dataset
    Format: text/tab-separated-values, 298 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 4
    facet.materialart.
    Unknown
    Environmental data and tsetse numbers per trap in protected and tsetse invested areas in Nigeria (2020)
    PANGAEA
    Details
    Publication Date: 2023-10-07
    Description: This data set includes data from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. The dataset includes temperature and relative humidity at the set traps, and the number of total trapped and dissected Glossina sp.
    Keywords: 234-CRNP-T001a; 234-CRNP-T001b; 234-CRNP-T002a; 234-CRNP-T002b; 234-CRNP-T003a; 234-CRNP-T003b; 234-CRNP-T004a; 234-CRNP-T004b; 234-CRNP-T005a; 234-CRNP-T005b; 234-CRNP-T006a; 234-CRNP-T006b; 234-CRNP-T007a; 234-CRNP-T007b; 234-CRNP-T008a; 234-CRNP-T008b; 234-CRNP-T009a; 234-CRNP-T009b; 234-CRNP-T010a; 234-CRNP-T010b; 234-CRNP-T011a; 234-CRNP-T011b; 234-CRNP-T012a; 234-CRNP-T012b; 234-CRNP-T013a; 234-CRNP-T013b; 234-CRNP-T014a; 234-CRNP-T014b; 234-CRNP-T015a; 234-CRNP-T015b; 234-CRNP-T016a; 234-CRNP-T016b; 234-CRNP-T017a; 234-CRNP-T017b; 234-CRNP-T018a; 234-CRNP-T018b; 234-CRNP-T019a; 234-CRNP-T019b; 234-CRNP-T020a; 234-CRNP-T020b; 234-IG-T001a; 234-IG-T001b; 234-IG-T002a; 234-IG-T002b; 234-IG-T003a; 234-IG-T003b; 234-IG-T004a; 234-IG-T004b; 234-IG-T005a; 234-IG-T005b; 234-KNP-T001a; 234-KNP-T001b; 234-KNP-T001c; 234-KNP-T001d; 234-KNP-T002a; 234-KNP-T002b; 234-KNP-T002c; 234-KNP-T002d; 234-KNP-T003a; 234-KNP-T003b; 234-KNP-T003c; 234-KNP-T003d; 234-KNP-T004a; 234-KNP-T004b; 234-KNP-T004c; 234-KNP-T004d; 234-KNP-T005a; 234-KNP-T005b; 234-KNP-T005c; 234-KNP-T006a; 234-KNP-T006b; 234-KNP-T006c; 234-KNP-T007a; 234-KNP-T007b; 234-KNP-T007c; 234-KNP-T007d; 234-KNP-T008a; 234-KNP-T008b; 234-KNP-T008c; 234-KNP-T009a; 234-KNP-T009b; 234-KNP-T009c; 234-KNP-T010a; 234-KNP-T010b; 234-KNP-T010c; 234-KNP-T011a; 234-KNP-T011b; 234-KNP-T012a; 234-KNP-T012b; 234-KNP-T013a; 234-KNP-T013b; 234-KNP-T014a; 234-KNP-T014b; 234-KNP-T015a; 234-KNP-T015b; 234-ONP-T001a; 234-ONP-T001b; 234-ONP-T001c; 234-ONP-T001d; 234-ONP-T002a; 234-ONP-T002b; 234-ONP-T002c; 234-ONP-T002d; 234-ONP-T003a; 234-ONP-T003b; 234-ONP-T003c; 234-ONP-T003d; 234-ONP-T004a; 234-ONP-T004b; 234-ONP-T004c; 234-ONP-T004d; 234-ONP-T005a; 234-ONP-T005b; 234-ONP-T005c; 234-ONP-T005d; 234-ONP-T006a; 234-ONP-T006b; 234-ONP-T006c; 234-ONP-T006d; 234-ONP-T007a; 234-ONP-T007b; 234-ONP-T007c; 234-ONP-T007d; 234-ONP-T008a; 234-ONP-T008b; 234-ONP-T008c; 234-ONP-T008d; 234-ONP-T009a; 234-ONP-T009b; 234-ONP-T009c; 234-ONP-T009d; 234-ONP-T010a; 234-ONP-T010b; 234-ONP-T010c; 234-ONP-T010d; 234-ONP-T011a; 234-ONP-T011b; 234-ONP-T011c; 234-T-T001a; 234-T-T001b; 234-T-T002a; 234-T-T002b; 234-T-T003a; 234-T-T003b; 234-T-T004a; 234-T-T004b; 234-T-T005a; 234-T-T005b; 234-T-T006a; 234-T-T006b; 234-T-T007a; 234-T-T007b; 234-T-T007c; 234-T-T008a; 234-T-T008b; 234-T-T008c; 234-T-T009a; 234-T-T009b; 234-T-T009c; 234-T-T010a; 234-T-T010b; 234-T-T010c; 234-T-T011a; 234-T-T011b; 234-T-T012a; 234-T-T012b; 234-T-T013a; 234-T-T013b; 234-T-T014a; 234-T-T014b; 234-T-T015a; 234-T-T015b; 234-T-T016a; 234-T-T016b; 234-T-T017a; 234-T-T017b; 234-T-T018a; 234-T-T018b; 234-T-T019a; 234-T-T019b; 234-T-T020a; 234-T-T020b; 234-T-T021a; 234-T-T021b; 234-T-T022a; 234-T-T022b; 234-YGR-T001a; 234-YGR-T001b; 234-YGR-T001c; 234-YGR-T002a; 234-YGR-T002b; 234-YGR-T002c; 234-YGR-T003a; 234-YGR-T003b; 234-YGR-T003c; 234-YGR-T004a; 234-YGR-T004b; 234-YGR-T004c; 234-YGR-T005a; 234-YGR-T005b; 234-YGR-T005c; 234-YGR-T006a; 234-YGR-T006b; 234-YGR-T006c; 234-YGR-T007a; 234-YGR-T007b; 234-YGR-T007c; 234-YGR-T008a; 234-YGR-T008b; 234-YGR-T008c; 234-YGR-T009a; 234-YGR-T009b; 234-YGR-T009c; 234-YGR-T010a; 234-YGR-T010b; 234-YGR-T010c; 234-YGR-T011a; 234-YGR-T011b; 234-YGR-T011c; 234-YGR-T012a; 234-YGR-T012b; 234-YGR-T012c; 234-YGR-T013a; 234-YGR-T013b; 234-YGR-T013c; 234-YGR-T014a; 234-YGR-T014b; 234-YGR-T014c; 234-YGR-T015a; 234-YGR-T015b; 234-YGR-T015c; 234-YGR-T016a; 234-YGR-T016b; 234-YGR-T016c; 234-YGR-T017a; 234-YGR-T017b; 234-YGR-T017c; 234-YGR-T018a; 234-YGR-T018b; 234-YGR-T018c; Animal African Trypanosomiasis in humans: An emerging zoonosis?; BICON; Comment; Cross River National Park, Nigeria, Africa; Event label; Glossina sp.; Humidity, relative; Ijah Gwari, Nigeria, Africa; Insecttrap, biconical; Kainji Lake National Park, Nigeria, Africa; Old Oyo National Park, Nigeria, Africa; Surface elevation; Temperature, air; The role of African trypanosomal trans-sialidases in the transmission of Trypanosoma congolense between the fly vector and life stock host suffering Nagana disease; Toungo Area, Nigeria, Africa; TRYPTS; TRYPZOO; Yankari Game Reserve, Nigeria, Africa
    Type: Dataset
    Format: text/tab-separated-values, 818 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 5
    facet.materialart.
    Unknown
    Collected tsetse tissue and tsetse species (Glossina sp.) collected in protected and tsetse invested areas in Nigeria (2020)
    PANGAEA
    Details
    Publication Date: 2023-10-07
    Description: This data set includes data from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field. Wings were removed and stored dry. Legs were immersed in a DNA-preservation agent. Salivary glands and ovaries were dissected and stored in a DNA-preservation agent. The whole gut was dissected, homogenized in the field and partially preserved for DNA analysis and used to investigate for sialidase activity. The data table summarizes the available tissue, sex and species information for each dissected specimen. Species collected were G. morsitans submorsitans, G. tachinoides, G. palpalis palpalis and several unidentified Glossina species.
    Keywords: 234-CRNP-T003b; 234-CRNP-T004b; 234-CRNP-T005b; 234-CRNP-T012b; 234-CRNP-T014b; 234-CRNP-T015b; 234-CRNP-T016b; 234-CRNP-T017b; 234-IG-T001b; 234-IG-T002b; 234-IG-T003b; 234-IG-T004b; 234-IG-T005b; 234-IG-T006b; 234-KNP-T001b; 234-KNP-T002b; 234-KNP-T002c; 234-KNP-T003b; 234-KNP-T003c; 234-KNP-T004b; 234-KNP-T004c; 234-KNP-T005c; 234-KNP-T006b; 234-KNP-T006c; 234-KNP-T007b; 234-KNP-T007c; 234-KNP-T008b; 234-KNP-T008c; 234-KNP-T009c; 234-KNP-T010b; 234-KNP-T010c; 234-ONP-T001b; 234-ONP-T002b; 234-ONP-T002c; 234-ONP-T003b; 234-ONP-T003c; 234-ONP-T003d; 234-ONP-T004b; 234-ONP-T005b; 234-ONP-T005c; 234-ONP-T006b; 234-ONP-T006c; 234-ONP-T006d; 234-ONP-T007b; 234-ONP-T007c; 234-ONP-T008b; 234-ONP-T008d; 234-ONP-T009b; 234-ONP-T009c; 234-ONP-T009d; 234-ONP-T010b; 234-ONP-T011b; 234-T-T007b; 234-T-T008b; 234-T-T009b; 234-T-T014b; 234-YGR-T001b; 234-YGR-T002c; 234-YGR-T004c; 234-YGR-T005c; 234-YGR-T006c; 234-YGR-T008c; 234-YGR-T009c; 234-YGR-T010c; 234-YGR-T011c; 234-YGR-T012c; 234-YGR-T013c; 234-YGR-T014c; 234-YGR-T015c; 234-YGR-T016c; 234-YGR-T018c; Animal African Trypanosomiasis in humans: An emerging zoonosis?; BICON; Collector; Comment; Cross River National Park, Nigeria, Africa; DATE/TIME; Dissector; Event label; Family; Feeding status; Ijah Gwari, Nigeria, Africa; Insecttrap, biconical; Kainji Lake National Park, Nigeria, Africa; Kingdom; Old Oyo National Park, Nigeria, Africa; Order; Reference/source; Sample ID; Sex; Species; Subsample ID; The role of African trypanosomal trans-sialidases in the transmission of Trypanosoma congolense between the fly vector and life stock host suffering Nagana disease; Tissues; Toungo Area, Nigeria, Africa; TRYPTS; TRYPZOO; Yankari Game Reserve, Nigeria, Africa
    Type: Dataset
    Format: text/tab-separated-values, 7326 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 6
    facet.materialart.
    Unknown
    Tryapanosoma prevalence in tsetse (Glossina sp.) gut collected in protected and tsetse invested areas in Nigeria (2020)
    PANGAEA
    Details
    Publication Date: 2023-10-07
    Description: This data set includes the results of nested PCR-analysis of tsetse (Glossina sp.) gut samples with primers described by Adams et.al. (2006) and Hamilton et al (2004) from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field. The gut samples were homogenized in the field. DNA was purified from preserved homogenized gut using the Qiagen Blood and Tissue Kit. Presence of trypanosomes was investigated by amplification of Internal Transcribed Spacer-1 (ITS1). Identification of the respective trypanosome species was done based on lengths polymorphism of the ITS1 region by estimating the approximate size of the DNA amplicon based on electrophoretic separation in agarose gels, as well as sequencing of selected ITS1 amplicons and additional glycosomal Glyceraldehyde-3-phosohate dehydrogenase.
    Keywords: Animal African Trypanosomiasis in humans: An emerging zoonosis?; The role of African trypanosomal trans-sialidases in the transmission of Trypanosoma congolense between the fly vector and life stock host suffering Nagana disease; TRYPTS; TRYPZOO
    Type: Dataset
    Format: application/zip, 7.7 kBytes
    Location Call Number Limitation Availability
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    DATA PANGAEA
  • 7
    facet.materialart.
    Unknown
    Sialidase assay and analysis of free sialic acid in tsetse (Glossina sp.) collected in protected and tsetse invested areas in Nigeria (2020)
    PANGAEA
    Details
    Publication Date: 2023-11-29
    Description: This data set includes results of a sialidase assay applied in tsetse gut tissue from a tsetse (Glossina sp.) collection carried out in Nigeria between 2014 and 2016. Traps were employed for one to three days in four different National Parks, one Game Reserve and two tsetse invested areas. Each tsetse was identified morphologically and dissected in the field, all tissues preserved and used for further analysis. The whole gut of each tsetse was homogenized in the field and partially preserved for DNA analysis and used to investigate for sialidase activity with a fluorescent substrate and a natural substrate. Analysis for sialidase activity was done by fluorescent measurement and reverse phase HPLC of processed gut samples.
    Keywords: 234-CRNP-T003b; 234-CRNP-T004b; 234-CRNP-T005b; 234-CRNP-T012b; 234-CRNP-T014b; 234-CRNP-T015b; 234-CRNP-T016b; 234-CRNP-T017b; 234-IG-T001b; 234-IG-T002b; 234-IG-T003b; 234-IG-T004b; 234-IG-T005b; 234-IG-T006b; 234-KNP-T001b; 234-KNP-T002b; 234-KNP-T002c; 234-KNP-T003b; 234-KNP-T003c; 234-KNP-T004b; 234-KNP-T004c; 234-KNP-T005c; 234-KNP-T006b; 234-KNP-T006c; 234-KNP-T007b; 234-KNP-T007c; 234-KNP-T008b; 234-KNP-T008c; 234-KNP-T009c; 234-KNP-T010b; 234-KNP-T010c; 234-ONP-T001b; 234-ONP-T002b; 234-ONP-T002c; 234-ONP-T003b; 234-ONP-T003c; 234-ONP-T003d; 234-ONP-T004b; 234-ONP-T005b; 234-ONP-T005c; 234-ONP-T006b; 234-ONP-T006c; 234-ONP-T006d; 234-ONP-T007b; 234-ONP-T007c; 234-ONP-T008b; 234-ONP-T008d; 234-ONP-T009b; 234-ONP-T009c; 234-ONP-T009d; 234-ONP-T010b; 234-ONP-T011b; 234-T-T007b; 234-T-T008b; 234-T-T009b; 234-T-T014b; 234-YGR-T002c; 234-YGR-T004c; 234-YGR-T005c; 234-YGR-T006c; 234-YGR-T008c; 234-YGR-T009c; 234-YGR-T010c; 234-YGR-T011c; 234-YGR-T012c; 234-YGR-T013c; 234-YGR-T014c; 234-YGR-T015c; 234-YGR-T016c; 234-YGR-T018c; Analytical method; Animal African Trypanosomiasis in humans: An emerging zoonosis?; BICON; Collector; Cross River National Park, Nigeria, Africa; Event label; Gear; Ijah Gwari, Nigeria, Africa; Insecttrap, biconical; Kainji Lake National Park, Nigeria, Africa; Old Oyo National Park, Nigeria, Africa; Reference/source; Relative fluorescence intensity, ratio; Sample ID; Sialic acid, free; Subsample ID; The role of African trypanosomal trans-sialidases in the transmission of Trypanosoma congolense between the fly vector and life stock host suffering Nagana disease; Toungo Area, Nigeria, Africa; Treatment; TRYPTS; TRYPZOO; Wavelength; Yankari Game Reserve, Nigeria, Africa
    Type: Dataset
    Format: text/tab-separated-values, 5968 data points
    Location Call Number Limitation Availability
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    DATA PANGAEA
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