Publication Date:
2011-09-14
Description:
For a time, membrane proteins proved intractable to X-ray crystallography. There were difficulties of overexpression and purification, multiple choices of solubilizing detergent that could only be decided by trial and error, unwillingness to crystallize, and often, poor diffraction from resultant crystals. However, the tide now seems to be turning, and in PNAS, Oldham and Chen (1) present an X-ray crystallography study of the entire WT maltose uptake protein from Escherichia coli, in which four different structures are described at commendable 2.2- to 2.4-Å resolution. Maltose uptake protein is a well-characterized model (2) for the large ABC transporter family, members of...
Print ISSN:
0027-8424
Electronic ISSN:
1091-6490
Topics:
Biology
,
Medicine
,
Natural Sciences in General
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