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  • 1
    Online Resource
    Online Resource
    Berlin, Heidelberg :Springer Berlin / Heidelberg,
    Keywords: Biochemistry. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (113 pages)
    Edition: 1st ed.
    ISBN: 9783540444237
    Series Statement: Reviews of Physiology, Biochemistry and Pharmacology Series ; v.151
    DDC: 612.01505
    Language: English
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: It has been postulated that elevated levels of matrix metalloproteinases (MMPs) and reactive oxygen species detected in fluids and biopsies of chronic wounds contribute to the failure of wound to heal by degrading essential growth factors, receptors and extracellular matrix proteins. This stimulated the development of a formulation containing metal ions (polyhydrated ionogen or PHI) and citric acid that reduced reactive oxygen species in cultures of polymorphonuclear leukocytes (J Wound Care 12:10, 2003). The effect of PHI on synthesis of MMPs was studied in cultures of tumor cells and human dermal fibroblasts. Dose response curves of PHI ranging from 0.02% to 10%(W/V) established no toxicity in cultures of human dermal fibroblasts after 24 hours of incubation with PHI at concentrations below 1.25%, as measured by MTT assay. Levels of MMP-2 activity measured by antibody capture assay were reduced approximately 80% in culture medium of Mor-86 glioma tumor cells after 24 hours of exposure to 1% PHI. These results suggest that PHI reduced the synthesis of MMP-2 protein, presumably by reducing transcription and/or translation of MMP-2 gene. Clinical case reports of chronic wounds treated by PHI dressing (DerMax) suggest that PHI treatment stimulated healing and reduced MMP-2 immunostaining in biopsies of the chronic wounds.Acknowledgment:  This research was supported by Greystone Medical Group.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK; Malden, USA : Blackwell Publishing Ltd/Inc.
    Wound repair and regeneration 13 (2005), S. 0 
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Bacterial growth within extended use wound dressings remains a problem. We have developed a superabsorbent, microbicidal dressing based on the permanent attachment of a cationic polymer (the quaternary ammonium compound diallyl dimethyl ammonium chloride, DADMAC) onto a range of physical substrates. Treated gauze (Sof-Wick™) absorbed 50x its weight in saline and reduced bacterial, viral and fungal growth by six logs in vitro (AATCC method 100), with no zone of inhibition or extractables, as shown in 〈link href="#f1"〉Figure 1 below. Bacterial kill occurs within minutes, and remains effective in 10% serum. Good hemostatic properties were demonstrated for treated gauze wound dressing using both a rat liver laceration model, and a renal artery transection model, showing equivalent efficacy to approved haemorrhage control dressings (Avitene, Surgicel, and Gel Foam) that do not claim microbicidal activity. Treated gauze passed cytotoxicity testing using rabbit eye and skin tests, as well as guinea pig dermal sensitization tests.〈figure xml:id="f1"〉1〈mediaResource alt="image" href="urn:x-wiley:10671927:WRR130216AL:image_n/WRR_130216al_f1.eps"/〉Gauze sponges inoculated with E. Coli and stained for bacterial presence. Left is control; right is NIMBUS treated. Note no zone of inhibition around treated gauze.Acknowledgment:  This research was supported by QuickMed Technologies.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK; Malden, USA : Blackwell Science Ltd/Inc.
    Wound repair and regeneration 12 (2004), S. 0 
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Introduction : Proinflammatory cytokines TNF-α, IL-1β, and matrix metalloproteinases (MMPs) are elevated in chronic wounds and their levels decrease during healing. TNF-α and IL-1β stimulate the production of MMPs which cause degradation of growth factors. Wound fluid from the V.A.C.® is of interest when investigating the mechanisms by which V.A.C.® therapy aids in wound healing. We hypothesize that the clinical improvement in healing of chronic wounds by the V.A.C.® system occurs by a shift from a chronic cytotoxic environment to a more conducive acute healing wound environment. This study aims at understanding the molecular and mechanistic consequences of V.A.C.® therapy on chronic wound healing. Methods : Wound fluid collected from two patients with Stage IV pressure ulcers at baseline, 24hours, 72hours, and 7 days following V.A.C.® therapy. Concentrations of TNF-α, IL-1β, MMP-2, -3, -9, TIMP-2, and total protein were determined using ELISA. Results : TNF-α levels in wound fluids decreased from baseline levels in two patients following 3 days of treatment. Similarly, levels of MMP-3, MMP-9 and TIMP-2 decreased from baseline following 3 days of treatment. There were no consistent trends in IL-1β & MMP-2. Conclusion : The preliminary results from this study suggest that improved wound healing with V.A.C.® therapy is related to decreased levels of proinflammatory cytokines, MMPs and TIMP-2 in wound fluid.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 39 (2001), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 38 (2000), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Electromagnetic surveys of a young, wholly sedimentary, strip barrier island (St. George Island) on Florida's Gulf Coast reveal a Dupuit-Ghyben-Herzberg fresh water lens with the thickest part located close to the ocean side of the island at both undeveloped and developed sites. This result is at odds with those obtained on other barrier beaches, where the lens is either approximately symmetric or skewed toward the lagoon side due to higher sea level and greater upper beach storage on the ocean side. The inferred morphology of the fresh water lens on St. George Island can be attributed to variations in hydrogeologic (e.g., hydraulic conductivity, presence of semipermeable confining layer) and/or boundary conditions (e.g., recharge, higher sea level on the lagoon side), but the effects of variations in these factors on the shape of the lens are not separable. We therefore use independent constraints on hydraulic conductivity (from tidal pumping analyses), hydrofacies distribution, and recharge patterns to assess the plausibility that lateral variations in various physical parameters produce the observed lens shape. Dune ridge topography, which may constitute a significant portion of the width of a narrow island, perturbs recharge and shallow ground water flow patterns and may contribute to the development of a thick fresh water lens on an island's ocean side. However, topographic variations alone cannot account for the anomalous form of the island's fresh water lens.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Physiology 52 (1990), S. 275-292 
    ISSN: 0066-4278
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Medicine , Biology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Pharmacology 36 (1996), S. 429-459 
    ISSN: 0362-1642
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Medicine , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical and experimental pharmacology and physiology 23 (1996), S. 0 
    ISSN: 1440-1681
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: 1. Discrete, non-adrenergic binding sites for imidazolines have been characterized in the brain and periphery. The 11 clonidine-preferring site is mainly distributed in the brain and brain stem, while the 12 idazoxan-preferring site is more widely distributed.2. The 11 site appears to be associated with modulation of blood pressure. Imidazolines act within the rostral ventrolat ral medulla to produce hypotension. The underlying signal transduction mechanism is poorly understood.3. The imidazolines clonidine and cirazoline inhibited nicotine-stimulated calcium entry into rat phaeochromocytoma (PC-12) cells by a non-adrenergic mechanism. This effect was not attributable to the stimulation of protein kinases.4. Similarly, clonidine and cirazoline inhibited nicotinestimulated inward currents into PC-12 cells. This inhibitory action was not altered by inhibitors of signal transducing G-proteins.5. Clonidine and cirazoline displaced the ion channel ligand [3H]-phencyclidine from nicotinic acetylcholine receptors, suggesting that these drugs act by direct blockade of the intrinsic ion channel of the nicotinic acetylcholine receptor.6. This ion channel-blocking activity represents a novel action of these imidazolines and may underlie some of the proposed physiological actions of 11 sites.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 325 (1987), S. 445-447 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] ICa was determined in differentiated N x G cells by the whole-cell clamp technique15, using Ba2+ as charge carrier. Outward currents were minimized by replacing K+ with Cs+. On step-depolarizations from -40 to OmV, /Ca had a density of 11.5± 2.4(xAcm~2 (mean±s.d.; n=46); the ...
    Type of Medium: Electronic Resource
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