ISSN:
1573-501X
Keywords:
combinatorial chemistry
;
delivery microreactors
;
drop-on-demand liquid
;
miniaturization
;
photolysis
;
silicon wafer
;
single bead synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Microstructured silicon wafers were employed as miniaturizedsolid-phase reaction vessels as well as miniaturized microtiter plates. Employing piezoelectric drop-on-demand liquidjets, a combinatorial library of 256 Peptides was synthesizedon single beads. The synthesis protocol was associated to thelocation in the silicon nano-well arrangement. Products werephotolytically cleaved in the same well that was used forsynthesis and subsequently interrogated for thrombin inhibitionin a homogeneous competition assay. The assay procedure wasbased on drop-on-demand liquid delivery and laser inducedfluorescence imaging. The novel format proved useful for theintegration of both synthesis and screening into one platform,a prerequisite for an iterative, evolutionary approach towardsdrug discovery.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1016287827310
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