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  • 1
    Electronic Resource
    Electronic Resource
    Chromosome doubling in Tripsacum: the production of artificial, sexual tetraploid plants (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A collection of embryogenic diploid calli of Tripsacum was established and treated with colchicine to induce chromosome doubling. Sections containing duplicated cells in calli were identified using flow cytometry and ploidy level was determined in the regenerated plantlets. Tetraploid plants from several origins were obtained. In contrast to wild polyploid plants, which show apomictic development, the regenerated tetraploid plants reproduced sexually. By hybridizing these plants with wild tetraploid apomicts, various populations were established; these will allow a study of the inheritance of apomixis in Tripsacum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00798.x
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  • 2
    Electronic Resource
    Electronic Resource
    Detection of the apomictic mode of reproduction in maize-Tripsacum hybrids using maize RFLP markers (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1198-1203 
    Details
    ISSN: 1432-2242
    Keywords: Diplospory ; RFLP ; Bulk-segregant analysis ; Genome similarity ; Intergeneric hybrids ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyploid plants in the genus Tripsacum, a wild relative of maize, reproduce through gametophytic apomixis of the diplosporous type, an asexual mode of reproduction through seed. Moving gene(s) responsible for the apomictic trait into crop plants would open new areas in plant breeding and agriculture. Efforts to transfer apomixis from Tripsacum into maize at CIMMYT resulted in numerou intergeneric F1 hybrids obtained from various Tripsacum species. A bulk-segregant analysis was carried out to identify molecular markers linked to diplospory in T. dactyloides. This was possible because of numerous genome similarities among related species in the Andropogoneae. On the basis of maize RFLP probes, three restriction fragments co-segregating with diplospory were identified in one maize-Tripsacum dactyloides F1 population that segregated 1∶1 for the mode of reproduction. The markers were also found to be linked in the maize RFLP map, on the distal end of the long arm of chromosome 6. These results support a simple inheritance of diplospory in Tripsacum. Manipulation of the mode of reproduction in maize-Tripsacum backcross generations, and implications for the transfer of apomixis into maize, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222943
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  • 3
    Electronic Resource
    Electronic Resource
    Chromosomal and embryological analyses in sexual x apomictic hybrids ofPanicum maximum Jacq. (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 153-156 
    Details
    ISSN: 1432-2242
    Keywords: Panicum maximum ; Embryology ; Apomixis ; Sexuality ; Polyploidization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cytological analyses in series of crosses between 7 sexual pistillate and 8 apomictic staminate parents of speciesPanicum maximum (Gramineae) are reported. Although these 15 progenitors were tetraploid (2n = 32), 2 dihaploids (2n = 16), 45 hexaploids (2n = 48) and 5 octoploids (2n = 64) were observed among 333 progeny plants. The role of unreduced gametes as the originators of polyploidy is discussed in relation to the so-called ‘elements of apomixis’. The 2 dihaploids appeared to be sexual while the hexaploids and octoploids were all apomictic. At the tetraploid level sexual and apomictic hybrids segregated in a ratio close to 1∶1. These results were then compared to those already obtained from studies on other tropical grasses and indicate a simple genetic determinism for gametophytic apomixis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279706
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  • 4
    Electronic Resource
    Electronic Resource
    Breeding Panicum maximum in Brazil. 1. Genetic resources, modes of reproduction and breeding procedures (1989)
    Springer
    Euphytica 41 (1989), S. 107-112 
    Details
    ISSN: 1573-5060
    Keywords: Panicum maximum ; guineagrass ; apomixis ; tropical grass breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Apomixis is widely distributed among tropical forage grasses, and has long been merely regarded as an impediment to breeding. Panicum maximum is presented as the first opportunity for Brazilian geneticists to develop and test original breeding schemes adapted to an apomictic species. A large and representative germplasm of P. maximum has been introduced and is currently being evaluated. Basic knowledges on biology and reproduction are also available, which demonstrate an easy manipulation of apomixis and sexuality. Several limiting traits have already been detected during evaluation, which justify breeding attempts. An ideal scheme is given to transfer new qualities to already selected varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022419
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