Description: Numerous long-term, free-air plant growth facilities currently explore vegetation responses to the ongoing climate change in northern latitudes. Open top chamber (OTC) experiments as well as the experimental set-ups with active warming focus on many facets of plant growth and performance, but information on morphological alterations of plant cells is still scarce. Here we compare the effects of in-situ warming on leaf epidermal cell expansion in dwarf birch, Betula nana in Finland, Greenland, and Poland. The localities of the three in-situ warming experiments represent contrasting regions of B. nana distribution, with the sites in Finland and Greenland representing the current main distribution in low and high Arctic, respectively, and the continental site in Poland as a B. nana relict Holocene microrefugium. We quantified the epidermal cell lateral expansion by microscopic analysis of B. nana leaf cuticles. The leaves were produced in paired experimental treatment plots with either artificial warming or ambient temperature. At all localities, the leaves were collected in two years at the end of the growing season to facilitate between-site and within-site comparison. The measured parameters included the epidermal cell area and circumference, and using these, the degree of cell wall undulation was calculated as an Undulation Index (UI). We found enhanced leaf epidermal cell expansion under experimental warming, except for the extremely low temperature Greenland site where no significant difference occurred between the treatments. These results demonstrate a strong response of leaf growth at individual cell level to growing season temperature, but also suggest that in harsh conditions other environmental factors may limit this response. Our results provide evidence of the relevance of climate warming for plant leaf maturation and underpin the importance of studies covering large geographical scales.

Description: Testate amoebae are an abundant and functionally important group of protists in peatlands, but little is known about the seasonal patterns of their communities. We investigated the relationships between testate amoeba diversity and community structure and water table depth and light conditions (shading vs. insolation) in a Sphagnum peatland in Northern Poland (Linje mire) in spring and summer 2010. We monitored the water table at five sites across the peatland and collected Sphagnum samples in lawn and hummock micro-sites around each piezometer, in spring (3 May) and mid-summer (6 August) 2010. Water table differed significantly between micro-sites and seasons (Kruskal–Wallis test, p = 0.001). The community structure of testate amoebae differed significantly between spring and summer in both hummock and lawn micro-sites. We recorded a small, but significant drop in Shannon diversity, between spring and summer (1.76 vs. 1.72). Strongest correlations were found between testate amoeba communities and water table lowering and light conditions. The relative abundance of mixotrophic species Hyalosphenia papilio, Archerella flavum and of Euglypha ciliata was higher in the summer.

Description: We reconstructed 3300 years (3500 BCE and 200 BCE) of the development history of the Rąbień peatland located in central Poland, using pollen, macrofossil, testate amoebae, Cladocera, Chironomidae and geochemistry. Central Europe, particularly Poland, is characterised by a transitional climate that is influenced by continental and Atlantic air masses, which makes this region very sensitive to climate change. Our results demonstrate the high potential of the Rąbień peat record to reconstruct palaeohydrological dynamics. The studied time interval is characterised by two pronounced dry periods: from ~ 2500 to ~ 1700 BCE and from ~ 700 to ~ 500 BCE, and two significant increases in the water table: from ~ 1000 to ~ 800 BCE and from ~ 500 to ~ 250 BCE. The timing of the wet shift at 600 BCE corresponds to wet periods at different sites in Central and Eastern Europe. Our investigation reveals a more complicated and complex than previously assumed set of climatic relationships in Europe between 3500 BCE and 200 BCE, which might be linked through complex teleconnections of atmospheric circulation patterns. Only reconstructions that are based on an understanding of current observations from peatlands and lake ecosystems may lead to a better interpretation of past climate changes.