ISSN:
1432-1424
Keywords:
Key words: NGF—Na channels—Pancreatic β-cells—cAMP—Reverse hemolytic plaque assay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract. Nerve growth factor (NGF) induces neuritelike process outgrowth in cultured adult pancreatic β cells. DbcAMP partially mimics this effect on cell morphology, and both compounds act synergistically to promote neuritelike process outgrowth. To determine if NGF- and dbcAMP-induced differentiation was accompanied by changes in β cell electrical activity, we studied the macroscopic Na current of adult rat β cells identified with the reverse hemolytic plaque assay and cultured for one week with these factors. After 5–7 days, β cells cultured in the presence of 2.5S NGF exhibited a 48% increase on the macroscopic Na current, which was due to an increase on Na current density. We did not observe changes on voltage dependence of current activation, nor on steady-state inactivation. Although dbcAMP also promotes changes on β cell morphology, it did not affect the Na current density.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002329900109
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