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  • 1
    Electronic Resource
    Electronic Resource
    Oxford UK : Blackwell Science Ltd.
    Journal of fish diseases 25 (2002), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Uptake of five chemical forms of erythromycin by adult Artemia salina (L.) (erythromycin phosphate – EP, erythromycin stearate – ES, erythromycin estolate – EE, erythromycin hydrate – EH and crystalline erythromycin – CE) was investigated in two trials. In each trial, final erythromycin concentration in Artemia tissue and survival after a 12-h bioencapsulation period were determined. In the first trial, Artemia tissue concentration after a 12-h bioencapsulation period was significantly (P 〈 0.05) affected by erythromycin form with ES (68.5 ± 3.3 μg mL−1, mean ± SEM) ≈ EH (61.2 ± 3.4 μg mL−1) 〉 CE (37.1 ± 10.7 μg mL−1) 〉 EP (16.4 ± 7.7 μg mL−1) 〉 control. In trial 2, Artemia tissue concentration was also significantly (P 〈 0.05) affected by erythromycin form with EE (111.4 ± 9.6 μg mL−1) 〉 CE (89.1 ± 1.7 μg mL−1) 〉 ES (78.9 ± 1.6 μg mL−1) 〉 EP (33.4 ± 5.2 μg mL−1) 〉 control. Survival was significantly affected by erythromycin form in trial 1 with EP=control (100 ± 0.0%) 〉 ES (74.4 ± 2.0%) 〉 CE (32.2 ± 0.3%) 〉 EH (8.8 ± 4.4%). In trial 2, survival was also significantly affected by erythromycin form with EP=control (100 ± 0.0%) 〉 ES (67.1 ±3.7%) 〉 CE (52.5 ± 7.7%) 〉 EE (5.0 ± 2.5%). Based on both uptake and survival, EP and ES appear to be appropriate compounds for bioencapsulation of erythromycin using live adult Artemia.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The potential to use adult Artemia to deliver erythromycin to first-feeding sockeye salmon, Oncorhynchus nerka (Walbaum), was investigated in three trials. In the first trial, first-feeding sockeye were fed live erythromycin enriched adult Artemia or pellets containing equal amounts of erythromycin for 35 days. At the end of the trial, tissue erythromycin concentration of the fish fed the live Artemia was significantly greater (P 〈 0.05, 25.52 ± 1.29 μg mL−1; mean ± SEM), than the tissue concentration of the fish fed the pellets (0.72 ± 0.01 μg mL−1). In the second trial, first-feeding sockeye were fed either live or freeze-dried bioencapsulated erythromycin (adult Artemia) or pellets containing erythromycin daily for 21 days. Mean daily erythromycin concentration in fish fed the freeze-dried Artemia, live Artemia, or pellets did not differ significantly. In the third trial, apparent erythromycin digestibility was determined. Significantly more (P 〈 0.05) erythromycin was retained by juvenile sockeye fed freeze-dried bioencapsulated erythromycin (98.3 ± 1.0%) compared with medicated pellets (89.2 ± 1.7%). Uptake of bioencapsulated erythromycin from adult Artemia (live or freeze-dried) appears to be greater than uptake from pellets. Freeze-dried and live Artemia were equally effective at delivery suggesting enriched freeze-dried adult Artemia could be produced into a highly palatable, consistent, off-the-shelf product.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of fish diseases 23 (2000), S. 0 
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Live adult brine shrimp, Artemia franciscana (Latreille), were enriched with erythromycin to determine if Artemia could accumulate therapeutic levels for subsequent feeding to young fish. Three trials were conducted to determine the erythromycin incorporation and survival rates of enriched Artemia when fed either liposomes containing erythromycin or various erythromycin suspensions. Erythromycin concentration in Artemia fed a liposome suspension was low (∼ 5 μg mL−1) relative to Artemia fed the direct suspension (〉 100 μg mL−1) over the same time period. When enriched with suspensions up to 1 g erythromycin L−1 sea water for 14 h, Artemia survival was not significantly affected (P 〉 0.05) relative to controls. Using a suspension of 1 g L−1, tissue erythromycin concentrations of 109 ± 16 μg erythromycin mL−1 Artemia homogenate (mean ± SEM) were achieved after 12 h. Concentrations above 170 μg mL−1 were obtained using suspensions of 2–5 g L−1, but Artemia survival significantly (P 〈 0.05) decreased.
    Type of Medium: Electronic Resource
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