ISSN:
1398-9995
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
A microfiberglass-based histamine assay (HRM) was compared with an automated flourometric histamine assay (HRA). Twenty-four with and 24 without a case history (CH) of milk and/or egg allergy were tested by HRM and HRA, skin prick test (SPT), and specific serum IgE (RAST). Six different concentrations of milk, egg, and anti-IgE to stimulate washed leukocytes (250 μ for HRA and whole blood samples (25 μ for HRM) in parallel. When we compared scores representing basophil senditivity, correlation coefficients (rs) were positive (r(anti-IgE))=0.88, r(egg) = 0.95, r(milk) = 0.88, P〈 0.001), but no significant correlation were found after found after exclusion of the negatives in both tests. In some individual dose-response curves, the scores obtained by HRM were shifted to higher allergen and anti-IgE concentrations. A high degree of concordance was found in positive and negative responses between the two: anti-IgE 91%, egg 92%, milk 86%. Finally, we found a good concordance between, on one other, CH, SPT, and RAST (HRM vs. CH/SPT/RAST) 92/82/82%; milk 89/74/67%. We conclude that HRM is in good qualitative, but poor quantitative, agreement with the autoanalyzer-based fluorometric histamine assay.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1398-9995.1993.tb02174.x
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