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  • 1
    Online Resource
    Online Resource
    General and Applied Aspects of Halophilic Microorganisms. (1991)
    New York, NY :Springer,
    Details
    Keywords: Halophilic microorganisms-Congresses. ; Electronic books.
    Description / Table of Contents: Proceedings of an FEMS-NATO ARW held in Alicant, Spain, September 17-22, 1989.
    Type of Medium: Online Resource
    Pages: 1 online resource (384 pages)
    Edition: 1st ed.
    ISBN: 9781461537304
    Series Statement: NATO Science Series A: Series ; v.201
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=6493085
    DDC: 576
    Language: English
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    GEOMAR EBL
  • 2
    Electronic Resource
    Electronic Resource
    Environmental genomics, the big picture? (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 231 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The enormous sequencing capabilities of our times might be reaching the point of overflowing the possibilities to analyse data and allow for a feedback on where to focus the available resources. We have now a foreseeable future in which most bacterial species will have an annotated genome. However, we know also that most prokaryotic diversity would not be included there. On the one hand, there is the problem of many groups not being easily amenable to culture and hence not represented in culture-centred microbial taxonomy. On the other hand, the gene pools present in one species can be orders of magnitude larger that the genome of one strain (selected for genome sequencing). Contrasting with eukaryotic genomes, the repertoire of genes present in one prokaryotic cell genome does not correlate stringently with its taxonomic identity. Hence gene catalogues from one environment might provide more meaningful information than the classical species catalogues. Metagenomics or microbial environmental genomics provide a different tool that gravitates around the habitat rather than the species. Such tool could be just the right way to complement ‘organismal genomics’. Its potential to advance our understanding of microbial ecology and prokaryotic diversity and evolution is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0378-1097(04)00006-0
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Unexpected diversity of small eukaryotes in deep-sea Antarctic plankton (2001)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 409 (2001), S. 603-607 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Phylogenetic information from ribosomal RNA genes directly amplified from the environment changed our view of the biosphere, revealing an extraordinary diversity of previously undetected prokaryotic lineages. Using ribosomal RNA genes from marine picoplankton, several new groups of bacteria and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/35054537
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Diversity of free-living prokaryotes from a deep-sea site at the Antarctic Polar Front (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 36 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: To contribute to the understanding of deep-sea planktonic communities, we explored the prokaryotic diversity of a 3000 m deep site at the Antarctic Polar Front using molecular methods. Bacterial 16S rDNA-amplified sequences corresponded to the as yet uncultivated groups SAR11, within the α-Proteobacteria, and SAR324, within the δ-Proteobacteria, as well as to the γ-Proteobacteria, Cytophagales, Planctomyces, Gram-positives, and the group of environmental sequences SAR406. Among them, γ-proteobacterial sequences were the most abundant and diverse. Within Archaea, and using six different primer sets for 16S rDNA amplification, only euryarchaeotal sequences were retrieved. Most of them clustered with the Thermoplasma-related marine groups II and III, but some corresponded to a recently described group of marine sequences emerging at the base of haloarchaea. Our data suggest that γ-Proteobacteria and Euryarchaeota may be dominant elements in terms of genetic diversity of the two prokaryotic domains in this deep-sea pelagic area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00840.x
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  • 5
    Electronic Resource
    Electronic Resource
    Spatial and temporal variation in marine bacterioplankton diversity as shown by RFLP fingerprinting of PCR amplified 16S rDNA (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 24 (1997), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We have applied a simple methodology based on restriction endonuclease digestion of the total bacterial and archaeal 16S rDNA amplified from sea water samples to compare the prokaryotic diversity present along a transect from coastal to offshore waters in the Mediterranean basin (from Barcelona to the Balearic Islands). Samples from the surface and deep-chlorophyll maximum (DCM) were obtained. Temporal variation during a short span was also investigated by repeating the sampling after 48 h. The patterns of digestion bands from the samples were compared to detect major changes in the bacterial or archaeal groups present. For bacteria the main difference was found between the free-living community and that retained by the eukaryotic filter and assumed to be particle attached. By contrast Archaea collected by the same means did not appear as a separate group. The other main variation was depth-related, with remarkably different communities at the surface, at the DCM, and a single sample taken at 400 m depth. On the other hand, the variation along the transect from the continental platform to an offshore (2000 m maximum depth) station was relatively small. Temporal (48 h) and small scale spatial (a few km) variation was minimal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1997.tb00420.x
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular evidence supporting the existence of two major groups in uropathogenic Escherichia coli (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 14 (1996), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract Molecular methods allow an extremely fine strain typing that can be used to establish the population structure of bacterial species. This methodology has been used to characterize a collection of 74 uropathogenic Escherichia coli obtained from three hospitals located in geographically distant towns in Spain, some representatives of the ECOR collection and other reference strains. Genomic DNA was analyzed by RAPD (Random Amplified Polymorphic DNA) that can characterize a bacterial strain to the level of defining individual clones. The 16S rDNA-23S rDNA spacers were amplified by PCR and submitted to restriction analysis. Finally, the presence or absence of G adhesins in Escherichia coli as well as the type of adhesin (three types are known) have been shown by PCR amplification followed by digestion with restriction enzymes. As expected a wide diversity was shown by RAPD and identical patterns were only found in the case of strains isolated from the same individual, an obvious case of relapse. Analysis of the spacers' restriction patterns showed the presence of two markedly differentiated clusters that we have named α and ß. Both RAPD and spacer restriction patterns originated similar clusters of strains showing a consistency in the evolution of the global genome with the sequence variation of the ribosomal spacers. Furthermore, most of the strains having G-adhesin, with only a few exceptions, corresponded to the α rRNA spacer group. The two spacer types detected were also consistent with some phenotypic markers such as sucrose and raffinose utilization. The α and β clusters could be intraspecific groups produced by partial sexual isolation or other barriers that are originating a divergent evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-695X.1996.tb00291.x
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Bacterial diversity in two coastal lagoons deduced from 16S rDNA PCR amplification and partial sequencing (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 18 (1995), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Amplification and sequence analysis of the 16S rRNA genes from DNA samples extracted directly from the environment allows the study of microbial diversity in natural ecosystems without the need for cultivation. In this study this methodology has been applied to two coastal lagoons. Activity and numbers of heterotrophic bacteria have indicated that, as expected, Prévost lagoon (located on the French Mediterranean coast) is more eutrophic than that of the Arcachon Bay (French Atlantic coast). Analysis of partial 16S rRNA gene sequences revealed that, in both environments, a relatively large number of clones related to Cytophaga/Flexibacter/Bacteroides as well as to α-Proteobacteria were found. One hundred percent similarity with the sequences of the data bases were not found for any of the more than a hundred clones studied, in fact for most clones maximum similarity was below 95% for the approx. 200 bases sequenced. Similarity was not higher with any of the sequences found for the 14 isolates (pure cultures) obtained from the same samples. Redundancy, i.e. number of identical sequences, was higher in the samples from Arcachon. In addition, sequences related to representatives of ten major phylogenetic branches of Bacteria were obtained from Prévost lagoon; however only five branches were represented by the data from Arcachon. These findings indicated a higher bacterial phylogenetic diversity in the Prévost lagoon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1995.tb00183.x
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    The contribution of halophilic Bacteria to the red coloration of saltern crystallizer ponds (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 36 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Analysis of the pigments extracted from solar saltern crystallizer ponds in Santa Pola near Alicante and on the Balearic island of Mallorca, Spain, showed that 5–7.5% of the total prokaryotic pigment absorbance could be attributed to a novel carotenoid or carotenoid-like compound. This unidentified pigment was identical to the sole pigment present in Salinibacter ruber, the only described member of a newly discovered genus of red halophilic Bacteria related to the Cytophaga-Flavobacterium-Bacteroides group. On the basis of fluorescence in situ hybridization experiments it has been shown that Salinibacter is an important component of the microbial community of Spanish saltern ponds. The red color of saltern crystallizer ponds may thus not only be due to red halophilic Archaea and to β-carotene-rich Dunaliella cells as previously assumed, but may contain a bacterial contribution as well. The Salinibacter pigment was not detected in samples collected from crystallizer ponds of the salterns of Eilat, Israel, and only traces of it may have been present in the Newark, CA, USA, salterns. The community structure of the prokaryote community inhabiting saltern crystallizers thus shows significant geographic variations. Polar lipid analyses of the biomass collected from the Santa Pola salterns showed that the total contribution of Salinibacter and other Bacteria to the total biomass was minor, the most important component of the community being halophilic Archaea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00832.x
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Antagonistic interactions among halobacteria due to halocin production (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 36 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract By using 3 different methods for the detection of antagonism, complex patterns of this type of interaction were evident within a group comprising 79 halobacterial strains, which included the most divergent types of culture collection strains available, as well as freshly isolated ones. The experiments carried out showed that such interactions are not due to phages, but to the effects of bacteriocin-like substances, known as halocins. With the exception of only 2 strains, all the remainder caused inhibition to a greater or lesser degree, showing a wide variety of activity spectra. Comparative numerical analysis of these spectra revealed the existence of at least 15 different groups of halocins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01691.x
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  • 10
    Electronic Resource
    Electronic Resource
    The ecology and taxonomy of aerobic chemoorganotrophic halophilic eubacteria (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 39 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract There exists a wide diversity of halophilic eubacteria with chemoorganotrophic-aerobic metabolism. Most of them have a more moderate salt response than halophilic archaebacteria, falling into the category of moderately halophilic bacteria. Although mostly isolated from salted food, their natural habitats are hypersaline waters of intermediate levels of salt concentration, and hypersaline soils. In hypersaline waters, the taxonomic groups found are the ones that also predominate in ocean waters, such as representatives of the genera Vibrio, Pseudomonas and Flavobacterium. However, in hypersaline soils, the taxonomic groups present are those typical of normal soils, such as Pseudomonas, Bacillus and Gram-positive cocci. The halophilic bacteria from soils are also more resistant to exposure to low salt concentrations than the organisms isolated from waters. Therefore, it seems that the general characteristics of the hypersaline environments drastically affect the types of halophilic bacteria present, and that the halophilic character has arisen in many phylogenetic groups of eubacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01837.x
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