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  • 1
    Electronic Resource
    Electronic Resource
    Crystallization and analysis of the subunit assembly and quaternary structure of imidazoleglycerol phosphate dehydratase from Saccharomyces cerevisiae (1995)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 51 (1995), S. 845-847 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Imidazoleglycerol phosphate dehydratase (IGPD) from Saccharomyces cerevisiae has been crystallized in the presence of a range of divalent cations using the hanging-drop method of vapour diffusion with ammonium sulfate or polyethylene glycol (PEG) 4000 as the precipitants. X-ray precession photographs have established that the crystals formed with ammonium sulfate (form A) belong to the space group F432, with cell parameter a = 177.5 Å and a single subunit in the asymmetric unit. A preliminary data set collected to 6 Å resolution on a two-detector San Diego Multiwire area detector has established that the crystals formed with PEG 4000 (form B) belong to either of the special pair of space groups I23 or I213, with cell parameter a = 131.0 Å. A self-rotation function has been calculated using these data and indicates that the cell axes show pseudo fourfold symmetry consistent with a dimer in the asymmetric unit in this crystal form. Light-scattering studies indicate that in the presence of Mn2+ and a number of other divalent cations IGPD undergoes assembly to a particle of molecular weight approximately 500 kDa. Given the subunit molecular weight of 23 kDa together with the symmetry of the crystals it would indicate that the most likely quaternary structure for this enzyme is based on a 24-mer in 432 symmetry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444995001569
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  • 2
    Electronic Resource
    Electronic Resource
    Crystallization of cytochrome b562 from Erwinia chrysanthemi (1997)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 53 (1997), S. 197-199 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Cytochrome b562 from Erwinia chrysanthemi has been crystallized using the hanging-drop vapour-diffusion method with ammonium sulfate as the precipitant. X-ray precession photographs show that the crystals formed belong to either of the enantiomorphic space groups P41212 or P43212 with the cell parameters a = b = 98.6 and c = 62.7 Å. Estimation of the crystal density and consideration of the possible values for Vm indicate that there is either a dimer or trimer in the asymmetric unit. Experiments using the synchrotron radiation source at the CCLRC Daresbury Laboratory have shown that the crystals diffract to at least 2.7 Å resolution. An analysis of the N-terminal sequence indicates that this cytochrome shows limited homology to the cytochrome b562 from E. coli. Determination of the structure will therefore allow analysis of the relationship between these two proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444996011201
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  • 3
    Electronic Resource
    Electronic Resource
    Altered intestinal development after jejunal ligation in fetal sheep (1993)
    Springer
    Virchows Archiv 423 (1993), S. 45-50 
    Details
    ISSN: 1432-2307
    Keywords: Fetus ; Small intestine ; Ultrastructure ; Intestinal atresia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Experimental obstruction of the fetal small intestine resulted in massive hypertrophy of the segment proximal to the site of obstruction. Villus morphology was grossly abnormal. Enterocytes developed many irregular features, most notably cytoplasmic extensions (pseudopods, or blebs) from their apical surface. Distal to the site of obstruction, morphological anomalies which resembled those seen after experimental oesophageal ligation were found. These included delayed disappearance of the apical endocytic network, disrupted or absent microvilli, glycogen accumulation and inappropriate cell extrusion. Proximal to the obstruction, where stasis of swallowed fluid occurs, distension and abnormal intestinal development ensues. Distal to the obstruction where the intestine develops in the absence of swallowed fluid, development is also abnormal. The anomalies resemble those noted after oesophageal ligation in utero, and possibly are the results of reduced cellular nutrition. These results suggest that fetal ingestion provides the developing gastrointestinal tract with an important stimulus for normal growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606431
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  • 4
    Electronic Resource
    Electronic Resource
    Basal lamina and other extracellular matrix produced by bovine granulosa cells in anchorage-independent culture (1995)
    Springer
    Cell & tissue research 282 (1995), S. 463-471 
    Details
    ISSN: 1432-0878
    Keywords: Basal lamina ; Basement membrane ; Ovary ; Follicle ; Granulosa cell ; Follicular fluid ; Collagen type IV ; Fibronectin ; Proteoglycan ; Ruthenium red ; Bovine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Bovine granulosa cells from 3–7 mm follicles were cultured without anchorage in soft agar/methylcellulose solution for 14 days, with or without 50 ng/ml basic fibroblast growth factor. The granulosa cells divided to form colonies of cells. These were analysed by light and electron microscopy, immunohistochemistry and Western immunoblotting. In approximately 20% of the colonies extracellular matrix was clearly visible at the light-microscope level. Ultrastructurally the matrix resembled a basal lamina 30–100 nm thick and was composed of tangled fibres or cords. Unidentified spherical structures of less than 50 nm diameter were sometimes present and attached to this basal lamina. The basal lamina of follicles had similar features, except that the basal lamina produced in vitro was a large aggregate of many convoluted layers. The cells produced collagen type IV and the cellular form of fibronectin. Intercellular areas not associated with basal lamina were identified. Ruthenium red staining revealed these areas to be rich in proteoglycan granules. Free granules were clustered near the cell surface, and the lumina of these areas were rich in fibres decorated with ruthenium red. This material did not resemble follicular fluid of antral follicles. Thus, granulosa cells in anchorage-independent cultures have a follicular cell morphology and secrete two distinct extracellular matrices, one similar to the follicular basal lamina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318878
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  • 5
    Electronic Resource
    Electronic Resource
    Basal lamina and other extracellular matrix produced by bovine granulosa cells in anchorage-independent culture (1995)
    Springer
    Cell & tissue research 282 (1995), S. 463-471 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Basal lamina ; Basement membrane ; Ovary ; Follicle ; Granulosa cell ; Follicular fluid ; Collagen type IV ; Fibronectin ; Proteoglycan ; Ruthenium red ; Bovine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Bovine granulosa cells from 3–7 mm follicles were cultured without anchorage in soft agar/methylcellulose solution for 14 days, with or without 50 ng/ml basic fibroblast growth factor. The granulosa cells divided to form colonies of cells. These were analysed by light and electron microscopy, immunohistochemistry and Western immunoblotting. In approximately 20% of the colonies extracellular matrix was clearly visible at the light-microscope level. Ultrastructurally the matrix resembled a basal lamina 30–100 nm thick and was composed of tangled fibres or cords. Unidentified spherical structures of less than 50 nm diameter were sometimes present and attached to this basal lamina. The basal lamina of follicles had similar features, except that the basal lamina produced in vitro was a large aggregate of many convoluted layers. The cells produced collagen type IV and the cellular form of fibronectin. Intercellular areas not associated with basal lamina were identified. Ruthenium red staining revealed these areas to be rich in proteoglycan granules. Free granules were clustered near the cell surface, and the lumina of these areas were rich in fibres decorated with ruthenium red. This material did not resemble follicular fluid of antral follicles. Thus, granulosa cells in anchorage-independent cultures have a follicular cell morphology and secrete two distinct extracellular matrices, one similar to the follicular basal lamina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050497
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    Paper (German National Licenses)
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