GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Document type
Keywords
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    Biomass, bioactivity and biodiversity: microbial ecology of the deep subsurface: analysis of ester-linked phospholipid fatty acids (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 20 (1997), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: To understand the ecology of microorganisms in deep subsurface sediments, it is necessary to utilize analyses which look at communities in situ since culturable organisms represent a small fraction of the extant microbiota. Application of the signature lipid biomarker (SLB) technology provides quantitative measures of viable biomass (abundance and distribution), community composition and insight into the nutritional physiological status of the community. With this analysis it was possible to define differences in the communities of different geologic horizons with differing geochemical and geophysical compositions. Although a number of different sites across the continental United States have been studied, the focus of this report is on a single site located in the semi-arid west in New Mexico state where late cretaceous (120–135 Myr) shales and sandstones could be sampled. Sediment cores from this site yielded ester-linked phospholipid fatty acids (PLFA) indicating the presence of viable microbiota as well as diglyceride fatty acids (DGFA) which described the recently dead or non-viable populations of microorganisms. Both the spatial distribution and the nature of PLFA and DGFA were examined under the premises of two hypotheses: (1) that microorganisms in unheated shales would be similar to modern near shore marine sediment organisms and would be absent from heated (volcanic) shales and (2) that different microbial communities would be detected in shales and sandstones as a result of environmental influences and restrictions both at the time of deposition and in subsequent diagenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1997.tb00322.x
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Microbial growth and resuscitation alter community structure after perturbation (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 17 (1995), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: An increase in the number of culturable organisms and a decrease in the diversity of recoverable microbiota have been reported in deep subsurface materials after storage perturbation. The magnitude of the microbial community shift in stored samples was more pronounced at 4°C compared to −20°C. Phospholipid fatty acid analyses and acridine orange direct counts indicated that biomass did not increase significantly throughout storage. Changes in the types of fatty acid methyl esters determined over the time course indicated that some of the microbial community shift was due to bacterial proliferation. However, the recovery of new bacterial types only after the storage process suggested that some of the increase in culturable cell count was due to the resuscitation of dormant microorganisms, possibly activated by some aspect of sampling, sample handling, and/or storage. Comparison of acridine orange direct counts with phospholipid and diglyceride fatty acid content suggested that much of the biomass may have been non-living at early time points; however, after 30 days of storage most of the bacterial biomass was viable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1995.tb00124.x
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Methanogenesis and methanotrophy within a Sphagnum peatland (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 18 (1995), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Methane production and consumption activities were examined in a Massachusetts peatland. Peat from depths of 5–35 cm incubated under anaerobic conditions, produced an average of 2 nmol CH4 g−1 h−1 with highest rates for peat fractions between 25–30 cm depth. Extracted microbial nucleic acids showed the strongest relative hybridization with a 16S rRNA oligonucleotide probe specific for Archaea with samples from the 25–30 cm depth. In aerobic laboratory incubations, the peat consumed methane with a maximum velocity of 67 nmol CH4 g−1 h−1 and a Ks of 1.6 μM. Methane consumption activity was concentrated 4–9 cm below the peat surface, which corresponds to the aerobic, partially decomposed region in this peatland. Phospholipid fatty acid analysis of peat fractions demonstrated an abundance of methanotrophic bacteria within the region of methane consumption activity. Increases in temperature up to 30°C produced an increase in methane consumption rates for shallow samples, but not for samples taken from depths greater than 9 cm. Nitrogen fixation experiments were carried out using 15N2 uptake in order to avoid problems associated with inhibition of methanotrophy. These experiments demonstrated that methane in peat samples did not stimulate nitrogen fixation activity, nor could activity be correlated with the presence of methanotrophic bacteria in peat fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.1995.tb00178.x
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Stability of a rhizosphere microbial community exposed to natural and manipulated environmental variability (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 38 (2001), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Phospholipid fatty acid (PLFA) and artificial neural network analyses were used to examine the composition of the Spartina alterniflora rhizosphere microbial community after exposure to manipulations in the field designed to alter the availability of host plant-derived and abiotic nutrient resources. We also tracked the experiments over a sufficient duration to observe significant natural variability in edaphic variables. We determined the PLFA composition of axenic S. alterniflora roots in order to distinguish differences in PLFAs that were due to changes in the rhizosphere microbiota from those only due to variability in plant root mass among samples. There were no significant changes in the PLFA profiles in response to experimental treatments and little change over the 8-week duration of the experiments. The microbial communities in the S. alterniflora rhizosphere did not respond dramatically to changing environmental conditions in the absence of major physical or chemical disruptions of the rhizosphere.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6941.2001.tb00883.x
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Comparison of phylogenetic relationships based on phospholipid fatty acid profiles and ribosomal RNA sequence similarities among dissimilatory sulfate-reducing bacteria (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 119 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Twenty-five isolates of dissimilatory sulfate-reducing bacteria were clustered based on similarity analysis of their phospholipid ester-linked fatty acids (PLFA). Of these, 22 showed that phylogenetic relationships based on the sequence similarity of their 16S rRNA directly paralleled the PLFA relationships. Desulfobacter latus and Desulfobacter curvatus grouped with the other Desulfobacter spp. by 16S rRNA comparison but not with the PLFA analysis as they contained significantly more monoenoic PLFA than the others. Similarly, Desulfovibrio africanus clustered with the Desulfovibrio spp. by 16S rRNA but not with them when analyzed by PLFA patterns because of higher monoenoic PLFA content. Otherwise, clustering obtained with either analysis was essentially congruent. The relationships defined by PLFA patterns appeared robust to shifts in nutrients and terminal electron acceptors. Additional analyses utilizing the lipopolysaccharide-lipid A hydroxy fatty acid patterns appeared not to shift the relationships based on PLFA significantly except when completely absent, as in Gram-positive bacteria. Phylogenetic relationships between isolates defined by 16S rRNA sequence divergence represent a selection clearly different from the multi-enzyme activities responsible for the PLFA patterns. Determination of bacterial relationships based on different selective pressures for various cellular components provides more clues to evolutionary history leading to a more rational nomenclature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06905.x
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Validation of signature polarlipid fatty acid biomarkers for alkane-utilizing bacteria in soils and subsurface aquifer materials (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 62 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Extractable cell membrane-derived polarlipid ester-linked fatty acids (PLFA) obtained from aerated soils gassed with methane or propane and from methane- and propane-oxidizing bacteria isolated from the soils were analyzed by capillary gas chromatography/mass spectrometry. Exposure of aerated soils to methane resulted in the formation of a high proportion of an unusual 18-carbon mono-unsaturated PLFA, 18:lw8c. High proportions of this fatty acid biomarker are found in monocultures from this soil grown in minimal media with methane. This PLFA has been previously established as associated with authentic type II methane-oxidizing bacteria. The microbiota in aerated soils exposed to hydrocarbons containing propane, formed a suite of PLFA characterized by high proportions of a 16-carbon mono-unsaturated acid, 16:lw6c, and an 18-carbon saturated fatty acid with an additional methyl branch at the 10 position, 10 Me 18:0. This PLFA pattern has been detected in several monocultures enriched from the soil with propane-amended minimal media. The correspondence of high proportions of these unusual mono-unsaturated PLFA in the isolated monocultures and in situ in the soils after stimulation with the appropriate hydrocarbon is a strong validation of the utility of these biomarkers in defining the community structure of the surface soil microbial community.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03656.x
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Characterization of the microbiology within a 21 m3section of rock from the deep subsurface (1993)
    Springer
    Microbial ecology 26 (1993), S. 145-159 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of aerobic chemoheterotrophic microorganisms within a 21 m3 section of deep subsurface rock was determined. Nineteen samples for microbiological analysis were aseptically taken by hand from the walls of a 400 m deep subsurface tunnel after an alpine miner created fresh rock faces 0.76, 1.52, 2.28, and 3.04 m into the tunnel wall. The direct counts were several orders of magnitude greater than viable counts in all samples. One of each morphologically distinct bacterial type from each sample was purified and analyzed for fatty acid methyl esters (FAME) using the Microbial Identification System (MIDI). Numbers of bacterial types, diversity, and equitability of recoverable microbial communities were the same or similar using either morphotype or FAME analyses as the basis for distinguishing between bacterial types. Twenty-nine genera (Euclidean distance of ⩽25) were found within the rock section, while 28 of the 210 bacterial types isolated were nonculturable under the growth regime required for cluster analysis. Most isolates clustered at the genus level with Arthrobacter, Gordona, and Acinetobacter. Two genera, containing 16 isolates, were unmatched to known organisms within the MIDI data base and clustered with other isolates at a Euclidean distance greater than 50. While some species (Euclidean distance ⩽10) were recovered from multiple sites within the rock section, most were found at 1–3 sites and usually without a definitive pattern of distribution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00177049
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Lipid components in anal scent sacs of three mongoose species (Helogale parvula, Crossarchus obscurus, Suricata suricatta) (1992)
    Springer
    Journal of chemical ecology 18 (1992), S. 1511-1524 
    Details
    ISSN: 1573-1561
    Keywords: Mongoose ; Helogale parvula ; Crossarchus obscurus ; Suricata suricatta ; anal gland ; lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Anal sac secretions of three social mongoose species (Helogale parvula, Crossarchus obscurus, andSuricata suricatta) were chemically analyzed by means of gas chromatography-mass spectrometry. Compounds with high molecular weights (greater than 250) were found to distinguish the three species. Differences in composition suggest a possible species-specific role in scent marking. MaleH. parvula had vitamin E present in the anal sac, whereas it was absent in the females. This suggests a possible sex-specific function of vitamin E inH. parvula. No differences in chemical composition were found between male and femaleS. suricatta. Ten compounds were found in femaleC. obscurus that were not present in the male.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00993225
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    facet.materialart.
    Unknown
    Geochemistry, microbiology and phospholipid fatty acid content of ODP Site 169-1036 sediments (2000)
    PANGAEA
    In:  Supplement to: Summit, Melanie; Peacock, Aaron D; Ringelberg, David; White, David C; Baross, John A (2000): Phospholipid fatty acid-derived microbial biomass and community dynamics in hot, hydrothermally influenced sediments from Middle Valley, Juan De Fuca Ridge. In: Zierenberg, RA; Fouquet, Y; Miller, DJ; Normark, WR (eds.) Proceedings of the Ocean Drilling Program, Scientific Results, College Station, TX (Ocean Drilling Program), 169, 1-19, https://doi.org/10.2973/odp.proc.sr.169.117.2000
    Details
    Publication Date: 2024-01-09
    Description: Phospholipid fatty acids were measured in samples of 60°-130°C sediment taken from three holes at Site 1036 (Ocean Drilling Program Leg 169) to determine microbial community structure and possible community replacement at high temperatures. Five of six samples had similar concentrations of phospholipid fatty acids (2-6 pmol/g dry weight of sediment), and biomass estimates from these measurements compare favorably with direct microscopic counts, lending support to previous microscopic measures of deep sedimentary biomass. Very long-chain phospholipid fatty acids (21 to 30 carbons) were detected in the sediment and were up to half the total phospholipid fatty acid measured; they appear to increase in abundance with temperature, but their significance is not known. Community composition from lipid analysis showed that samples contained standard eubacterial membrane lipids but no detectable archaeal lipids, though archaea would be expected to dominate the samples at high temperatures. Cluster analysis of Middle Valley phospholipid fatty acid compositions shows that lipids in Middle Valley sediment samples are similar to each other at all temperatures, with the exception of very long-chain fatty acids. The data neither support nor deny a shift to a high-temperature microbial community in hot cores, so at the present time we cannot draw conclusions about whether the microbes observed in these hot sediments are active.
    Keywords: Ocean Drilling Program; ODP
    Type: Dataset
    Format: application/zip, 2 datasets
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
  • 10
    facet.materialart.
    Unknown
    (Table T1) Geochemical and microbiological characteristics of ODP Site 169-1036 sediments (2000)
    PANGAEA
    Details
    Publication Date: 2024-01-09
    Keywords: 169-1036A; 169-1036B; 169-1036C; Ammonium; Bacteria, abundance; Calcium; Chlorine; Dead Dog vent field, North Pacific Ocean; DEPTH, sediment/rock; DRILL; Drilling/drill rig; DSDP/ODP/IODP sample designation; Escanaba Trough, North Pacific Ocean; Event label; Fatty acids, very long chain; Joides Resolution; Leg169; Magnesium; Ocean Drilling Program; ODP; pH; Phospholipid fatty acids; Sample code/label; Sulfate; Temperature, in rock/sediment
    Type: Dataset
    Format: text/tab-separated-values, 72 data points
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    DATA PANGAEA
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...