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  • 1
    Electronic Resource
    Electronic Resource
    Glutamate dehydrogenase regulation in callus cultures of Nicotiana plumbaginifolia: effect of glucose feeding and carbon source starvation on the isoenzymatic pattern (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 14 (1991), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. In callus cultures of Nicotiana plumbaginifolia, the activity of glutamate dehydrogenase was repressed by glucose, whereas, on the contrary, carbon and energy source deprivation induced a remarkable increase in specific activity. Definition of these two opposite types of response was made possible by the use of glycerol as a non-repressing carbon source: in this condition, glutamate dehydrogenase activity reached an intermediate level, which was similar to the derepressed values of activity obtainable when cultures were allowed to exhaust the glucose supply in the medium. Isoelectric focusing analysis revealed the existence of three different isoenzymatic patterns which could be correlated to the three different levels of specific activity: repressed (glucose), induced (carbon starvation) and intermediate (glycerol). Repression affected mainly the four more cathodic bands which were predominant in non-repressed conditions. The possible catabolic role of these isoenzymes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1991.tb01533.x
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of glucose on glutamate dehydrogenase and acid phosphatase and its reversal by cyclic adenosine 3′:5′-monophosphate in single cell cultures of Asparagus officinalis (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 60 (1984), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cell cultures of Asparagus officinalis L. ev. “Argenteuil” were used to study the effect of glucose on the synthesis of glutamate dehydrogenase and acid phosphatase. Both the enzymes are progressively repressed by increasing the glucose concentration in the culture medium. Furthermore, synthesis of glutamatc dehydrogenase and acid phosphatase in cells grown in the presence of high glucose concentrations is derepressed by the addition of the cyclic adenosine 3′:5′-monophosphate to the culture medium. These data support the hypothesis that also in cells from higher plants a regulatory mechanism exists with some similarities to the “catabolite repression” mechanism operating in several prokaryotes and lower eukaryotes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1984.tb04250.x
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  • 3
    Electronic Resource
    Electronic Resource
    Isozyme gene markers in the dioecious species Asparagus officinalis L. (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 613-618 
    Details
    ISSN: 1432-2242
    Keywords: Asparagus officinalis ; Isoenzymes ; Marker genes ; Sex-linked inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Extracts from phylloclads of Asparagus officinails were electrophoretically analyzed for isozyme polymorphism. Fourteen enzyme systems were examined using four buffer systems: seven enzymes (acid phosphatase, catalase, glutamate-oxaloacetate transaminase, isocitrate dehydrogenase, malate dehydrogenase, peroxidase, and 6-phosphogluconate dehydrogenase) exhibited clear and consistent banding patterns. Isozyme polymorphism was studied in seven pairs of male and female doubled haploids and in their male F1s. Segregation of polymorphic loci was examined in the backcross progenies and was found to be consistent with a simple Mendelian inheritance in all cases, except for three anodical peroxidases, where two factors have been hypothesized. No linkage could be found between isozyme markers that were segregating in the same cross, but association was demonstrated between one malate dehydrogenase locus and the sex determining genes. The availability of isozyme markers may be useful in breeding and, in particular, the localization of one malate dehydrogenase locus on the sex chromosomes may be helpful in mapping the sex genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226726
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  • 4
    Electronic Resource
    Electronic Resource
    A genetic map of Asparagus officinalis based on integrated RFLP, RAPD and AFLP molecular markers (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1083-1089 
    Details
    ISSN: 1432-2242
    Keywords: Key words Asparagus ; Dioecious species ; Genetic map ; Molecular markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An integrated genetic map of the dioecious species Asparagus officinalis L. has been constructed on the basis of RFLP, RAPD, AFLP and isoenzyme markers. The segregation analysis of the polymorphic markers was carried out on the progeny of five different crosses between male and female doubled-haploid clones generated by anther culture. A total of 274 markers have been organized to ten linkage groups spanning 721.4 cM. Since the haploid chromosome number of asparagus is ten, the established linkage groups probably represent the different chromosomes; however, the only group associated with a specific chromosome is the one which includes sex, whose determinant genes have been located on chromosome 5. A total of 33 molecular markers (13 RFLPs, 18 AFLPs, 2 RAPDs and 1 isoenzyme) have been located on this chromosome. The closest marker to the sex determinant is the AFLP SV marker at 3.2 cM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050995
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  • 5
    Electronic Resource
    Electronic Resource
    Linkage Arrangement of RFLP loci in progenies from crosses between doubled haploid Asparagus officinalis L. clones (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 124-128 
    Details
    ISSN: 1432-2242
    Keywords: Asparagus officinalis L ; Genetic map RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A preliminary genetic map of the dioecious species Asparagus officinalis L. (2n = 20) has been constructed on the basis of restriction fragment length polymorphism (RFLP) and isozyme marker data. With DNA samples digested with either EcoRI or HindIII 61 out of 148 probes (41%) identified RFLPs in six families of doubled haploid lines obtained through anther culture. A higher level of polymorphism (65%) was observed when a single family was screened for RFLPs using six distinct restriction enzymes. Segregation analysis of the BC progenies (40–80 individuals) resulted in a 418-cM extended map comprising 43 markers: 39 RFLPs, three isozymes and one morphological (sex). These markers are clustered in 12 linkage groups and four of them exhibited significant deviations from the expected 1∶1 ratio. One isozyme and three RFLP markers were assigned to the sex chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221005
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  • 6
    Electronic Resource
    Electronic Resource
    Analysis of rho mutability in Saccharomyces cerevisiae (1980)
    Springer
    Molecular genetics and genomics 177 (1980), S. 589-595 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Erythromycin induced mutability of the cytoplasmic factor rho in S. cerevisiae does not depend only on the participation of its mitochondrial target (formally a mitochondrial protein synthesis component) to the replication of the mitochondrial DNA. The relative increase from the spontaneous background in the percentage of RD mutants accumulated in the presence of the drug is the same in mmc and MMC strains whereas the total number of RD mutants accumulated is about tenfold higher in mmc strains. Mutations at the mitochondrial locus that controls the sensitivity to Erythromycin reduce both the Erythromycin induced and the spontaneous mutability of rho in mmc strains indicating that the gene products of nuclear (MMC) and mitochondrial (ERYs) determinants interact in determining the fidelity of a “replicative complex” for the mitochondrial DNA. The fact that ERYR allelesaare able to suppress the pet-ts associated mutability of rho but not the RD conditional trait of these mutants makes evident that the pet-ts gene-products have an additional role beside the enzymatic or the biosynthetic ones. Since the pet-ts genes are not directly involved in the metabolism of mitochondrial DNA we hypothesize that they provide for some structures that contribute to ensure the proper conformation of a “replicative complex” for mitochondrial DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272668
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  • 7
    Electronic Resource
    Electronic Resource
    Analysis of rho mutability in Saccharomyces cerevisiae (1980)
    Springer
    Molecular genetics and genomics 177 (1980), S. 581-588 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two additional types of nuclear determinants involved in the control of spontaneous mutability of rho in S. cerevisiae have been identified: mmc and the pet-ts 1, 2, 10, 52 and 53 genes. These genes in their mutated recessive form increase at various extents the number of respiratory deficient cytoplasmic “petite” mutants accumulated. The gene mmc does not affect the respiratory activity and is not temperature-dependent whereas the pet-ts genes determine at the non permissive temperature a respiratory deficient phenotypes even if they affect the mutability of rho at the permissve and at the non permissive temperature. The data here reported suggest that a “replicative complex” exists for the mitochondrial DNA. It is in the purpose of this paper to deal with the relative contrition that mmc and pet-ts gene products have in ensuring the fidelity of this “replicative complex”.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272667
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