ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
The monoclonal antibody MoAb-VκIIIb binds a cross-reactive idiotopic (CRI) determinant on light (L) chains encoded by the VκIIIb subgroup A27a (Humkv325) gene segment. The aim of this study was to localize the MoAb-VκIIIb CRI. Mutational analyses involving region exchanges between a CRI-positive VκIIIb chain and a CRI-negative Vκ1 chain indicate that the MoAb-VκIIIb CRI is located in framework region (FR) 3 of A27a (Humkv325) encoded L chains. CRI-positive kappa chains unpaired with a heavy (H) chain are reactive with MoAb-VκIIIb, indicating that the CRI is located on the kappa chain alone without involvement of H chain residues. Combinatorial antibodies composed of non-parental L and H chain pairings are reactive with MoAb-VκIIIb only when the L chain is A27a (Humkv325) encoded. The CRI, therefore, is not readily perturbed by H chain interactions. When the FR3 from a CRI-positive kappa chain replaced the FR3 in a CRI-negative lambda chain, the determinant was no longer detectable with MoAb-VκIIIb. It is possible, therefore, to exchange regions between kappa chains from different families and retain the CRI structure, however the determinant is lost when placed in a more foreign background such as a lambda chain. These data more precisely define the interaction between MoAb-VκIIIb and its CRI, and indicate that there are limits within which antibody FRs can be shuffled and still retain their native structural features.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1365-3083.1996.d01-319.x
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