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  • 1
    Electronic Resource
    Electronic Resource
    Drought signal transduction in plants (1996)
    Springer
    Plant growth regulation 20 (1996), S. 105-110 
    Details
    ISSN: 1573-5087
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Water deficit is one of the most common environmental limitations of crop productivity by affecting growth through alterations in metabolism and gene expression. The mechanisms involved in drought perception and signal transduction pathways are poorly understood. The participation of the plant hormone abscisic acid (ABA) has been well established. ABA levels increase when there are changes in the environment that result in cellular dehydration. Different approaches have been taken to understanding the molecular responses to desiccation and how ABA regulates gene expression. Recent efforts have identified particular topics of importance in the dissection of the signal transduction pathway which are summarized as follows: physiological approaches: identification of signalling molecules. Genetic approaches: the use of mutants, and Molecular approaches: promoter analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024006
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization, subcellular localization and nuclear targeting of casein kinase 2 from Zea mays (1999)
    Springer
    Plant molecular biology 40 (1999), S. 199-211 
    Details
    ISSN: 1573-5028
    Keywords: casein kinase 2 ; gene structure ; phosphorylation ; nuclear localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) α subunit using the previously described αCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100–150 bp. The sequence of the exons is 100% homologous to the sequence of the αCK2-1 cDNA. Southern hybridization of total genomic DNA from maize embryos with αCK2 cDNA indicated that the αCK2-1 gene is part of a multigenic family. We also isolated a new embryo cDNA clone coding for an αCK2-2 subunit. We studied the regulation of the enzyme in embryos at the mRNA level, at the protein level and by activity testing. By using immunocytochemistry the CK2 protein was localized in several types of cells of mature embryos. Particularly strong signals were visible in the cytoplasm of epidermis and meristematic cells. Decoration of nuclei of root cortex and scutellum cells was also observed suggesting that CK2 can shift from the cytoplasm into nuclei in specific cell types. We examined whether CK2 contained specific protein domains which actively target the protein to the nucleus by using in-frame fusions of the maize CK2 α subunit to the reporter gene encoding β-glucuronidase (GUS) which were assayed in transiently transformed onion epidermal cells. Analysis of chimeric constructs identified one region containing a nuclear localization signal (NLS) that is highly conserved in other αCK2 proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006196530079
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    Paper (German National Licenses)
    Fulltext
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