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  • 1
    Electronic Resource
    Electronic Resource
    Purification and properties of α-1,4-glucan phosphorylase from the red seaweed Gracilaria sordida (Gracilariales) (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: α-1,4-Glucan phosphorylase (EC 2.4.1.1) from the red seaweed Gracilaria sordida(Harv.) W. Nelson was adsorbed onto starch-Sepharose 6B and Sephacryl S-300 under specified conditions. The algal enzyme was purified to homogeneity by these two steps. A molecular weight of 97.4 kDa was observed on SDS-polyacrylamide gel electrophoresis under reducing conditions, while the native molecular weight was 240 kDa asrevealed by 8-25% native gradient gel electrophoresis or 245 kDa by gel filtration. The pI of the enzyme was 5.4. It had a Km of 227, 264, 285, and 453 μg ml-1, respectively, towards glycogen, amylopectin, amylose, and maltodextrin. The enzyme activity was inhibited by cyclohexaamylose, ADP-glucose, and UDP-glucose. In contrast to other plant sources, cell-free extracts of G. sordida contained only one form of phosphorylase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb02122.x
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  • 2
    Electronic Resource
    Electronic Resource
    A Brominating and Hydroxylating Peroxidase from the Red Alga Cystoclonium purpureum (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 37 (1976), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A particulate enzyme displaying peroxidase activity has been extracted from the red alga Cystoclonium purpureum (Huds.) Batt. The enzyme preparation was shown to contain ferri-protoporphyrin IX through its formation of formic acid hemochromogen with absorption maxima at 399, 402, 555 and 600 nm. The preparation catalyses the formation of 3-bromo-p-hydroxybenzyl alcohol from p-hydroxybenzyl alcohol in the presence of H2O2 and NaBr at pH = 5.4. The formation of 3-bromo-p-hydroxybenzyl alcohol was measured by gas chromato-graphy-mass spectrometry. At pH = 6.7, 4,5-di-hydroxybenzyl alcohol was formed. Addition of homogentisic acid stimulated the formation of dihydroxybenzyl alcohol and suppressed the brominating reaction. Iodide inhibits the enzyme. The results are consistent with a two-site model of the enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1976.tb01864.x
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of a Cytokinin, 6-(3 Methyl-2-butenylamino) purine, in Sea Water and the Effect of Cytokinins on Brown Algae (1973)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 28 (1973), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Using combined gas chromatography and mass spectrometry a growth-promoting, organic component of sea water has been quantitatively estimated and identified as the cytokinin 6-(3 methyl-2-butenylamino) purine. Additions of this cytokinin, kinetin or sea water from the Fucus-Ascophyllum zone increased the growth of Ectocarpus confervoides and Pylaiella litoralis in artificial sea water. Additions of kinetin to bacteria-free cultures of Ectocarpus fasciculatus also initiated the production of upright ectocarpoid filaments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1973.tb01158.x
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  • 4
    Electronic Resource
    Electronic Resource
    A stress-induced oxidative burst in Eucheuma platycladum (Rhodophyta) (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 92 (1994), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A hurst of hydrogen peroxide has been found in the red macroalga Eucheuma platycladwn Schmitz as a response to mechanical stress. After exposure of pieces of thalli (2 cm) broken from the plant and stirred with a magnetic bar an oxidative burst was registered, as measured by luminol dependent chemiluminescence (LDC). The burst was totally inhibited by cataluse (EC 1.11.1.6). showing the generation of H2:O2; Ten g of seaweed in 300 ml sea water caused a maximal medium concentration of LDC corresponding to 7 u.M H2O2; The burst decayed after about 30 min. The decay is probably caused by increased catalase aciivity of the sea water. due to leakage of catalasc from the seaweed. Addition of NaN3 caused a dramatic increase in LDC. probably due to inhibition of catalase. Similar bursts of active oxygen, involving active oxygen species such as O2, H2O2 and OH. have been reported as pan of the hypcrsensitive reaction in some higher plants, e.g. tobacco. potato and soybean. Exposure of plants or cell suspension cultures to some pathogenic bacteria, fungi, inorganic elicitors or physical damage causes an oxidalive burst that is often followed by necrosis. The production ot active oxygen is thought to he a first defence against invading pathogens. We assume that the oxidative burst from E. platycladum is of a defensive nature, providing a protection against grazers and pathogenic organisms. To our knowledge this is the first repoil of an oxidalive burst from seaweeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb08830.x
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  • 5
    Electronic Resource
    Electronic Resource
    The Effect of Activated Charcoal on Tissue Cultures: Adsorption of Metabolites Inhibiting Morphogenesis (1978)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 43 (1978), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cell suspensions of Daucus carota and Haplopappus gracilis and callus suspensions of Allum cepa var. proliferum were grown in media with and without activated charcoal. Differentiation occurred in those Daucus and Allium cultures that contained charcoal. It was shown by mass spectrometry that the media without charcoal contained high amounts of phenylacetic acid and p-OH-benzoic acid (Daucus), 2,6-OH-benzoic acid (Allium) and benzoic acid, pelargonic acid and caprylic acid (Haplopappus), whereas the media with activated charcoal did not. It was also shown that p-OH-benzoic acid had inhibitory effects on the embryogenesis in Daucus cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1978.tb01575.x
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  • 6
    Electronic Resource
    Electronic Resource
    Marine Brown Algae Requiring Vitamin B12 (1969)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 22 (1969), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The brown algae Lithosiphon pusillus, Ectocarpus fasciculatus and Pylaiella litoralis were cultivated in bacteria-free cultures in artificial sea water, ASP 6F. The growth was tested with different additions of vitamins and other metabolites. Lithosiphon pusillus and Ectocarpus fasciculatus were found to require vitamin B12 for optimal growth.The zoospores of Pylaiella litoralis, when cultured in medium Asp 6F with kinetin added, had an absolute requirement for vitamin B12.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1969.tb07455.x
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  • 7
    Electronic Resource
    Electronic Resource
    Bromine, Bromophenols and Floridorubin in the Red Alga Lenormandia prolifera (1979)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 46 (1979), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The pigment floridorunin was localized to the cuticle of the red alga Lenormandia prolifera (C. Ag.) J. Agardh by x-ray micro-analysis in Ihe transmission electron microscope and by its colour reactions in the light microscope. The pigment was set free from the cell wall by a pectinase. Bromine was also identified in the chloro-plasts. the middle lamellae, the intercellulars and the pore plugs of the alga. The content of bromophenols in the alga increases with increasing age. The cell walls of old plants are stratified and their outer parts are apparently shed. The bromophenols could have a function as regulators of the epiphytes or the shedding of parts of the outer cell wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1979.tb06543.x
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  • 8
    Electronic Resource
    Electronic Resource
    The Demand for Iodine and Bromine of three Marine Brown Algae grown in Bacteria-free Cultures (1969)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 22 (1969), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Three marine brown algae have been cultivated with different additions of iodine and bromine in bacteria-free cultures. Ectocarpus jasciculatus appeared to have an absolute demand for iodine and was inhibited by a concentration of 64 μmol of KJ per 1. Lithosiphon pusillus had the best growth in the highest concentration tested (64 μmol/1) but there was always some growth in the series without iodine. Additions could be made either as inorganic iodine or as organically bound iodine. Additions of KJ to a culture medium consisting of vitamin-free Asp 6 F with B12 (1 μg/1) and kinetin (20 μmol/1) remarkably increased the growth of the zoospores of Pylaiella litoralis. Lithosiphon pusilius proved to be indifferent to bromide additions in media containing KJ. In media lacking KJ addition of 1 μmol of KBr per 1 is stimulating but higher concentrations of KBr are inhibiting. The inhibiting effect is overcome by iodide addition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1969.tb07423.x
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  • 9
    Electronic Resource
    Electronic Resource
    Ectocarpus fasciculatus : Marine Brown Alga requiring Kinetin (1968)
    [s.l.] : Nature Publishing Group
    Nature 218 (1968), S. 776-776 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To obtain Ectocarpus fasciculatus showing its normal habitus I had to use kinetin which influences both rate of growth and appearance3. In two experiments I found kinetin to have a growth stimulating effect (Tables 1 and 2). Ectocarpus fasciculatus appeared to have normal morphology in ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/218776a0
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  • 10
    Electronic Resource
    Electronic Resource
    Cytokinin-like activity in sea water from theFucus-Ascophyllum zone (1972)
    Springer
    Cellular and molecular life sciences 28 (1972), S. 111-112 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Les résultats indiquent la présence de cytokinines dans l'eau de mer de la zone deFucus-Ascophyllum. Des cultures de tissus deNicotiana ont été employés pour prouver l'activité des cytokinines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01928297
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