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  • 1
    Electronic Resource
    Electronic Resource
    Vanilla viruses in the South Pacific (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 42 (1993), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Surveys for viruses affecting Vanilla fragrans and V. tahitensis carried out in Cook Islands, Fiji, Niue, Tonga and Vanuatu detected cymbidium mosaic potexvirus and odontoglossum ringspot tobamovirus in all countries; vanilla necrosis potyvirus in Fiji, Tonga and Vanuatu; and vanilla mosaic potyvirus in Cook Islands, Fiji and Vanuatu. For all these viruses the surveys extended the previously known distribution in the South Pacific. Other, unreported potyviruses and rhabdovirus-like particles were detected from Fiji and Vanuatu. Symptoms associated with potyvirus infection (leaf distortion, sunken chlorotic patches, stem necrosis and vine die-back) were found in a majority of plantings in Fiji, Tonga and Vanuatu; in Fiji and Tonga there were instances where up to 50% of the plants in farmers’ plots were affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1993.tb01479.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The occurrence and effects of barley yellow dwarf virus in maize in SW England (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 33 (1984), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: During 1975 and 1976 barley yellow dwarf virus was isolated, using the aphid Rhopalosiphum padi (L.) from five maize cultivars in the Exeter area. The isolates produced interveinal flecking, often accompanied by leaf reddening, in the maize cultivar Anjou 210 and several other susceptible cultivars. ln 1976 visual assessment of six commercial maize plantings indicated natural infections ranging from 11 to 14% of plants, whilst infection in a disease observation plot ranged from 0.8 to 27.5%. Population counts for the three aphid vectors Sitobion avenae (F.), Metopolophium dirhodum (W.) and Rhopalosiphum padi (L.), associated with the crop, corresponded closely with the Rothamsted Insect Survey results from Starcross, Devon.The maize cultivars Anjou 210 and De Kalb 202 were grown in experimental plots with individual rows containing 0, 25, 50, or 100% barley yellow dwarf-infected plants. Infection caused severe stunting of Anjou 210 but had less effect on De Kalb 202. Dry matter yield losses of 50% for Anjou 210 and 27% for De Kalb 202 were recorded; there were also significant changes (p〈0.05) in dry matter content, metabolisable energy, digestible crude protein and water soluble carbohydrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1984.tb02874.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Severe stunting of Vanilla tahitensis in French Polynesia caused by Cucumber mosaic virus (CMV), and the detection of the virus in V. fragrans in Reunion Island (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 50 (2001), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2001.00579.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Taxonomy of Wisteria vein mosaic virus and extensions to its host range and geographical distribution (2003)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 52 (2003), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Wisteria mosaic, a serious disease of Wisteria spp. in horticultural production in many parts of the world, is caused by a virus, Wisteria vein mosaic virus (WVMV). This paper reports the presence of the virus in a new host, Wisteria venusta, and a new geographical distribution, New South Wales, Australia. A partial sequence (1329 nucleotides) of this isolate of WVMV was obtained, which represents the first available sequence data for the virus. Alignment of the nucleotide and predicted amino acid sequences with those of members of the Potyviridae showed closest identity with viruses of the Potyvirus genus. The predicted amino acid sequence has one open reading frame, open at the 5′ end, corresponding to part of the nuclear inclusion b protein and the capsid protein, followed by a 251-nucleotide untranslated region and a polyadenylated tail at the 3′ end.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2003.00798.x
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  • 5
    Electronic Resource
    Electronic Resource
    Characterization of a strain of Apple stem grooving virus in Actinidia chinensis from China (2003)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 52 (2003), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A new strain of Apple stem grooving virus (ASGV) has been identified in Actinidia chinensis imported from China. The leaves of these plants exhibited a variety of symptoms including interveinal mottling, chlorotic mosaics and ringspots. Capillovirus-like particles were observed under the electron microscope, and the virus could be mechanically transmitted to a range of herbaceous indicators. The virus was detected using ELISA with antisera raised against ASGV. Sequencing of the virus revealed that it had more than 95% amino acid identity with ASGV in the putative coat and movement proteins. From the morphological, transmission, serological and molecular evidence, it was concluded that the virus is a strain of ASGV. It is not known how this strain of ASGV is transmitted, other than by grafting, nor is it known what effect the virus has on the growth of infected vines. The Actinidia-infecting strain of ASGV does not occur in New Zealand, and infected plants will not be released from quarantine. The detection methods used during the research will assist quarantine and the safe movement of breeding material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2003.00857.x
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  • 6
    Electronic Resource
    Electronic Resource
    N-terminal polyhedrin sequences and occludedBaculovirus evolution (1981)
    Springer
    Journal of molecular evolution 17 (1981), S. 329-333 
    Details
    ISSN: 1432-1432
    Keywords: Baculovirus ; Polyhedrin ; N-terminal amino acid sequences ; Molecular evolution ; Virus evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A phylogenetic tree for occluded baculoviruses was constructed based on the N-terminal amino acid sequence of occlusion body proteins from six baculoviruses including three lepidopteran nuclear polyhedrosis viruses (NPVs), [two unicapsid (Bombyx mori andOrgyia pseudotsugata) and one multicapsid (Orgyia pseudotsugata)]; one granulosis virus (Pieris brassicae); and NPVs from a hymenopteran (Neodiprion sertifer) and a dipteran (Tipula paludosa). Amino acid sequence data for theB. mori NPV were from a report by Sere-bryani et al. (1977) and that for theO. pseudotsugata NPVs were reported previously by us (Rohrmann et al. 1979). The other N-terminal amino acid sequences are presented in this paper. The phylogenetic relationships determined based on the molecular evolution of polyhedrin were also investigated by antigenic comparisons of the proteins using a solid phase radioimmune assay. The results indicate that the lepidopteran NPVs are the most closely related of the above group of viruses and are related to these viruses in the following order:N. sertifer NPV,P. brassicae granulosis virus, andT. paludosa NPV. These data, in conjunction withBaculovirus distribution and evidence concerning insect phylogeny, suggest that theBaculovirus have an ancient association with insects and may have evolved along with them.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01734354
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  • 7
    Electronic Resource
    Electronic Resource
    Nucleotide sequence, serology and symptomatology suggest that vanilla necrosis potyvirus is a strain of watermelon mosaic virus II (1993)
    Springer
    Archives of virology 129 (1993), S. 93-103 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Vanilla necrosis potyvirus (VNV) is the cause of significant losses to the South Pacific islands vanilla crop. The gene for the coat protein of VNV has been cloned and sequenced. Comparison of this gene with other potyviral coat protein sequences revealed 97% nucleotide sequence homology (98% amino acid homology) to a US isolate of watermelon mosaic virus II (WMV-II), 93% nucleotide sequence homology (96% amino acid homology) to an Australian isolate of WMV-II and 81% nucleotide sequence homology (88% amino acid homology) to soybean mosaic virus-N (SMV-N). Serological analysis, by Western blot and ELISA, confirmed the close relationship between VNV and WMV-II. Furthermore, a limited host range determination found VNV and WMV-II able to infect the same series of test plants. However, symptoms differed significantly on three test species demonstrating that VNV and WMV-II are not identical in biological properties. We suggest that VNV be renamed WMV-II (Tonga).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01316887
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  • 8
    Electronic Resource
    Electronic Resource
    Rapid vegetative multiplication in Colocasia esculenta (L) Schott (taro) (1998)
    Springer
    Plant cell, tissue and organ culture 55 (1998), S. 223-226 
    Details
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Initial multiplication of taro was achieved by dividing corms into pieces, each with several buds, and planting them in sterile potting mix. Surface planting and decapitation of the new primary meristem on each sucker forced additional shoot meristems to grow. Meristems of six cultivars were cultured on a modified Murashige and Skoog medium plus TDZ. In experiments with the cultivar Niue, explants cultured on modified MS medium plus 2.6 μM (0.6 mg L-1 TDZ grew more vigorously than on media including BA. Transfer to 4.3 μM (1.0 mg L-1 TDZ subsequently gave a 15–25 fold increase in production of plantlets per four week culture period compared to four-fold with BAP. The two stage propagation technique described in this paper is an efficient way to produce taro plants for commercial planting or the production of pathogen-tested material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1017156112466
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  • 9
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    Recombinant expression of the coat protein of Botrytis virus X and development of an immunofluorescence detection method to study its intracellular distribution in Botrytis cinerea [FUNGAL] (2012)
    Society for General Microbiology (SGM)
    Details
    Publication Date: 2012-10-12
    Description: Botrytis cinerea is infected by many mycoviruses with varying phenotypical effects on the fungal host, including Botrytis virus X (BVX), a mycovirus that has been found in several B. cinerea isolates worldwide with no obvious effects on growth. Here we present results from serological and immunofluorescence microscopy (IFM) studies using antiserum raised against the coat protein of BVX expressed in Escherichia coli fused to maltose-binding protein. Due to the high yield of recombinant protein it was possible to raise antibodies that recognized BVX particles. An indirect ELISA, using BVX antibodies, detected BVX in partially purified virus preparations from fungal isolates containing BVX alone and in mixed infection with Botrytis virus F . The BVX antiserum also proved suitable for IFM studies. Intensely fluorescing spots (presumed to be virus aggregates) were found to be localized in hyphal cell compartments and spores of natural and experimentally infected B. cinerea isolates using IFM. Immunofluorescently labelled sections through fungal tissue, as well as fixed mycelia grown on glass slides, showed aggregations of virions closely associated with fungal cell membranes and walls, next to septal pores, and in hyphal tips. Also, calcofluor white staining of mature cell walls of virus-transfected Botrytis clones revealed numerous cell wall areas with increased amounts of chitin/glycoproteins. Our results indicate that some BVX aggregates are closely associated with the fungal cell wall and raise the question of whether mycoviruses may be able to move through the wall and therefore not be totally dependent on intracellular routes of transmission.
    Print ISSN: 0022-1317
    Electronic ISSN: 1465-2099
    Topics: Medicine
    Published by Society for General Microbiology (SGM)
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