Note: Front Cover -- The Centrosome in Cell Replication and Early Development -- Copyright Page -- Contents -- Contributors -- Preface -- Chapter 1. The Centrosome and Parthenogenesis -- I. Introduction -- II. Toward a Functional Description of the Centrosome -- III. The Centrosome Organelle versus Self-Assembled Centers -- IV. Centrosome Reproduction and the Parthenogenetic Activity of the Centrosome -- V. Conclusion -- References -- Chapter 2. y-Tubulin -- I. Centrosomes and Microtubule Nucleation -- II. y-Tubulin and the Tubulin Superfamily of Proteins -- III. Distribution of y-Tubulin in Cells -- IV. Studies of y-Tubulin Function -- V. y-Tubulin and Microtubule Assembly: Current Knowledge and Unanswered Questions -- VI. Concluding Remarks -- References -- Chapter 3. y-Tubulin Complexes and Their Role in Microtubule Nucleation -- I. Introduction -- II. Biochemical and Structural Studies of y TuRC and y TuSC -- III. Evidence That y TuRC Is the Major Microtubule Nucleator at the Centrosome -- IV. The Mechanism of Microtubule Nucleation -- V. Concluding Remarks -- References -- Chapter 4. y-Tubulin of Budding Yeast -- I. Introduction -- II. Tubulins of Yeast -- III. Interactions between Tubulins -- IV. The g -Tubulin Complex in Yeast -- V. Future Directions -- References -- Chapter 5. The Spindle Pole Body of Saccharomyces cerevisiae: Architecture and Assembly of the Core Components -- I. Introduction -- II. SPB Duplication and Assembly of the Mitotic Spindle -- III. Structure of the SPB -- IV. SPB Core Components -- V. Assembly of the Core SPB Components -- VI. Transcriptional Regulation of Core SPB Components -- VII. Homologs of Spindle Pole Components in Other Organisms -- VIII. Future Directions -- References -- Chapter 6. The Microtubule Organizing Centers of Schizosaccharomyces pombe -- I. Introduction. , II. Cytoplasmic Microtubule Organizing Centers -- III. The Spindle Pole Body -- IV. Molecular Characterization of SPB Components -- V. Perspectives -- References -- Chapter 7. Comparative Structural, Molecular, and Functional Aspects of the Dictyostelium discoideum Centrosome -- I. Introduction -- II. Comparative Morphology of the Dictyostelium Centrosome -- III. Comparative Analysis of Dictyostelium Centrosome Duplication -- IV. Comparative Molecular Biology of the Dictyostelium Centrosome -- V. Conclusions -- References -- Chapter 8. Are There Nucleic Acids in the Centrosome? -- I. Introduction -- II. Do Centrioles or Centrosomes Contain DNA? -- III. Do Centrosomes Contain RNA? -- IV. Conclusions -- References -- Chapter 9. Basal Bodies and Centrioles: Their Function and Structure -- I. Introduction to Centrioles and Basal Bodies -- II. The Structure of Centrioles and Basal Bodies -- III. The Function of Basal Bodies and Centrioles as Revealed by Mutational Analysis -- IV. Basal Body/Centriole Replication -- References -- Chapter 10. Centriole Duplication and Maturation in Animal Cells -- I. Overview -- II. Centriole Lineage Patterns -- III. Structural Features of Centriole Maturation and Duplication -- IV. Centriolar Duplication, Maturation, and the Cell Cycle -- V. Centriolar Maturation and Centriolar Function -- VI. Ciliogenesis and Cell Cycle -- VII. Future Developments and Directions -- References -- Chapter 11. Centrosome Replication in Somatic Cells: The Significance of the G1 Phase -- I. Introduction -- II. The Cell Cycle in Somatic Mammalian Cells -- III. Electron Microscopic Descriptions of Centrosome Doubling, and the Cellular Events of Centrosome Replication -- IV. Spindle Pole Body Doubling in Yeast Cells -- V. Events in G1 That Trigger Centrosome Replication in Somatic Mammalian Cells -- VI. Summary -- References. , Chapter 12. The Coordination of Centrosome Reproduction with Nuclear Events during the Cell Cycle -- I. Introduction -- II. The Events of Centrosome Reproduction -- III. Controls for Centrosome Reproduction -- IV. Coordination of Centrosome Reproduction with Nuclear Events in the Cell Cycle -- References -- Chapter 13. Regulating Centrosomes by Protein Phosphorylation -- I. Introduction -- II. Protein Phosphorylation: A Major Theme in Cell Cycle Control -- III. Centrosome and Spindle Pole Body Dynamics through the Cell Cycle -- IV. In Control of Centrosome Duplication -- V. Regulating Microtubule Nucleation -- VI. Establishing a Bipolar Mitotic Spindle -- VII. Creating the Zygotic Centrosome -- VIII. Loss of Phosphorylation Control: Cancer Implications -- IX. Perspectives: Into the Next Millennium -- References -- Chapter 14. The Role of the Centrosome in the Development of Malignant Tumors -- I. Introduction -- II. Centrosome Defects and Abnormal Mitoses in Cancer -- III. Amplified Centrosomes and Aneuploidy -- IV. Excess Pericentriolar Material Is Associated with High Frequency of Abnormal Mitoses -- V. Centrosome-Associated Kinases and Cancer -- VI. Tumor Suppressor Proteins and the Centrosome -- VII. Maintenance of Cell and Tissue Polarity Minimizes Tumor Aggression in Model Systems -- VIII. Conclusions -- References -- Chapter 15. The Centrosome-Associated Aurora/Ipl-like Kinase Family -- I. Introduction -- II. The Aurora and Ipl-like Family: Structure, Regulation and Substrates -- III. Summary -- References -- Chapter 16. Centrosome Reduction during Mammalian Spermiogenesis -- I. Introduction -- II. Overview of Mammalian Spermiogenesis -- III. Stages of Centrosome Reduction during Spermiogenesis -- IV. Conclusions -- References. , Chapter 17. The Centrosome of the Early C. elegans Embryo: Inheritance, Assembly, Replication, and Developmental Roles -- I. Why C. elegans? -- II. Origin and Replication of the Zygotic Centrosomes -- III. The Central Role of the Centrosome-Pronucleus Complex in Specifying AP Polarity -- IV. Nuclear Positioning -- V. Concluding Remarks -- References -- Chapter 18. The Centrosome in Drosophila Oocyte Development -- I. Introduction -- II. The Germarium: Centrosomes Are Anchored to Achieve Polarized Cell Divisions -- III. The Oocyte MTOC -- IV. Meiosis -- V. Summary -- References -- Chapter 19. The Centrosome in Early Drosophila Embryogenesis -- I. Introduction -- II. Ultrastructure -- III. Composition -- IV. Duplication and Separation -- V. Fertilization and the First Mitotic Division -- VI. Precortical Divisions -- VII. Cortical Divisions -- References -- Chapter 20. Centrosome Maturation -- I. Introduction -- II. Centrosome Maturation -- III. Centrosome Structure and Microtubule Nucleation Potential -- IV. The Role of the Centriole -- V. The Procentrosome -- VI. Paternal versus Maternal Centrosomes -- VII. Summary -- References -- Index -- Contents of Previous Volumes.

Note: Cover -- Contents -- Contributors -- Preface -- Chapter 1. Purification and Reconstitution of Drosophila y-Tubulin Complexes -- I. Introduction -- II. Isolation of γ -Tubulin-Containing Complexes from Drosophila Embryo Extracts -- III. γTuSC Reconstitution in Sf9 Cells -- IV. Functional Assays for Isolated γTuSC or γTuRC -- V. Concluding Remarks -- References -- Chapter 2. Using Rapid Freeze and Freeze-Substitution for the Preparation of Yeast Cells for Electron Microscopy and Three-Dimensional Analysis -- I. Introduction -- II. How Rapid Freezing and Freeze-Substitution Work -- III. Rapid Freezing Techniques -- IV. Freeze-Substitution Protocols -- V. Embedding Protocols -- VI. Sectioning and Imaging -- VII. Immunolabeling for Electron Microscopy -- VIII. Three-Dimensional Modeling of Spindle Pole Bodies and Other Organelles -- IX. Conclusion -- References -- Chapter 3. Digital Fluorescence Microscopy of Cell Cytoplasts with and without the Centrosome -- I. Introduction -- II. Experimental Procedures -- References -- Chapter 4. Methods for the Study of Pericentrin in Centrosome Assembly and Function -- I. Introduction -- II. Pericentrin Localization to Centrosomes -- III. Pericentrin Recruitment to Centrosomes -- IV. Obtaining Enriched Fractions of Pericentrin -- V. Artificial Elevation of Pericentrin Levels -- VI. Pericentrin Staining to Detect Centrosome Defects in Tumor Tissues and Cell Lines -- References -- Chapter 5. Molecular Dissection of Yeast Spindle Pole Bodies by Two Hybrid, in Vitro Binding, and Co-purification -- I. Introduction -- II. The Two-Hybrid Approach -- III. Immuno- and Affinity Precipitation of Spindle Pole Body Components -- IV. In Vitro Binding of Spindle Pole Body Proteins -- References -- Chapter 6. Genetic Analysis of Yeast Spindle Pole Bodies -- I. Introduction -- II. Dosage-Dependent Suppressor Screen. , III. Extragenic Suppressor Screen -- IV. Synthetic Lethal Screen -- V. Synthetic Dosage Lethality -- VI. Conclusions -- References -- Chapter 7. Methods for the Study of Centrosomes in Drosophila during Embryogenesis -- I. Introduction -- II. Materials -- III. Methods -- References -- Chapter 8. Methods for Identification of Centrosome-Associated Proteins -- I. Introduction -- II. Preparation of Centrosomes and Anticentrosomal Antibodies -- III. Molecular Cloning of cDNA Encoding Centrosome-Associated Proteins -- IV. Evaluation of Clone Specificity -- V. Identification of a Novel 135-kDa Centrosomal Protein, Cep135 -- References -- Chapter 9. Reconstitution of Centrosome Microtubule Nucleation in Drosophila -- I. Introduction -- II. Preparation of Extracts and Centrosomes from Drosophila Embryos -- III. In Vitro Reconstitution of Microtubule Nucleation by Salt-Inactivated Centrosomes -- IV. Conclusions -- References -- Chapter 10. Reconstitution of Centrosome Microtubule Nucleation in Spisula -- I. Introduction -- II. Preparations -- III. Disassembly and Reassembly of Microtubule Nucleation Potential -- IV. Fractionation of Extracts -- V. Summary -- References -- Chapter 11. In Vitro Approaches for the Study of Microtubule Nucleation at the Fission Yeast Spindle Pole Body -- I. Introduction -- II. Protocol for Permeabilization -- III. Protocol for Microtubule Nucleation -- IV. Results -- References -- Chapter 12. Gamma Tubulin and Microtubule Nucleation in Mammalian Cells -- I. Introduction -- II. Interference with γ -Tubulin Function in Mammalian Cells Abrogates Microtubule Nucleation -- III. Overexpression of γ -Tubulin in Mammalian Cells Causes Ectopic Microtubule Nucleation -- IV. Conclusion -- References -- Chapter 13. Gamma Tubulin in Plant Cells -- I. Introduction -- II. Microtubule Arrays in Plant Cells. , III. Methods for Cloning Plant γ -Tubulin cDNAs and Genes -- IV. Methods for Localizing γ -Tubulin in Plant Cells -- V. Functional Studies of γ -Tubulin in Plants -- VI. Future Prospects -- References -- Chapter 14. Centrosomes and Parthenogenesis -- I. Introduction -- II. Parthenogenetic Test in Xenopus -- III. Coupling between Egg Cell Cycle and Centrosome Duplication Pathway -- IV. Species Barrier Specificity -- V. Active or Inactive Centrosomes from the Same Species -- VI. Prospects -- References -- Chapter 15. In Vitro Approaches for the Study of Molecular Motors in Aster Formation -- I. Introduction -- II. Microtubule Aster Formation -- III. Examination of Microtubule Motor Function in Aster Assembly -- IV. Concluding Remarks -- References -- Chapter 16. Methods for the Study of Centrosome-Independent Spindle Assembly in Xenopus Extracts -- I. Introduction -- II. Preparation of Xenopus Egg Extract and Necessary Reagents -- III. Microtubule Polymerization Assays in Extract -- IV. Spindle Assembly Assays -- V. Studying Proteins Involved in Spindle Assembly -- VI. Methods to Observe Spindle Assembly -- VII. Conclusions -- References -- Chapter 17. Methods for the Study of Centrosome Reproduction in Mammalian Cells -- I. Introduction -- II. Induction of Centrosome Overproduction in Cultured Mammalian Cells -- III. Preparation of Extracts from Hydroxyurea-Arrested Chinese Hamster Ovary Cells -- IV. Analysis of Centrosome Protein Function Using Mammalian Cell Extracts and Salt-Stripped Centrosome Remnants -- V. Conclusions and Prospects -- References -- Chapter 18. Centrosome Reproduction in Xenopus Lysates -- I. Introduction -- II. Reagents and Equipment -- III. Methods -- References -- Chapter 19. Centrosome Reproduction in Vitro: Mammalian Centrosomes in Xenopus Lysates -- I. Introduction -- II. Logic of the Assay -- III. Duplication Assay. , IV. Major Pitfalls -- V. Discussion -- VI. Prospects -- References -- Chapter 20. Identification of Centrosome Kinases -- I. Introduction -- II. Subcellular Localization of Previously Characterized Protein Kinases -- III. Isolation of Novel Centrosome Kinases -- IV. Functional Analysis of Centrosome Kinases -- V. Identifying Substrates for Centrosome Kinases -- VI. Perspectives -- References -- Chapter 21. Methods for the Analysis of Centrosome Reproduction in Cancer Cells -- I. Introduction -- II. Tissue Procurement and Storage -- III. Preparation and Storage of Xenopus Egg Extract -- IV. Reaction Mixture Composition and Use -- V. Experimental Design and Data Analysis -- VI. Summary -- VII. Appendices -- References -- Chapter 22. Isolation of Centrosomes from Dictyostelium -- I. Introduction -- II. Isolation of Dictyostelium Centrosomes -- III. Analysis of Isolated Dictyostelium Centrosomes -- IV. Generation of Monoclonal Antibodies against Dictyostelium Centrosomes -- V. Conclusions -- References -- Index -- Volumes in Series.