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  • 1
    Electronic Resource
    Electronic Resource
    Quantitative changes in cytoplasmic and microsomal proteins associated with aluminium toxicity in two cultivars of winter wheat (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 14 (1991), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Seedlings of two sister lines of hard red winter wheat, Tx78 (Al-sensitive) andTx84 (Al-tolerant), were given a 24-h pulse of Al sufficient to irreversibly inhibit growth in the sensitive but not the tolerant cultivar. Proteins were then extracted from root tips of both cultivars, separated by 2D-PAGE, and quantified using computer analysis of gels. The objective of the work was to determine if the major changes in protein expression associated with Al stress were cultivar-specific, if the changes correlated with Al tolerance, and if they occurred in a specific fraction of cell proteins. Of the approximately 600 proteins examined, 14 cytoplasmic proteins and eight microsomal proteins were induced or enhanced by Al treatment in one or both cultivars, while nine cytoplasmic and 12 microsomal proteins were diminished or repressed. Among the 43 proteins significantly altered by Al treatment, three cytoplasmic proteins, and no microsomal proteins, were induced or enhanced solely in the-AI-tolerant cultivar. Al affected the programme of formation of both cytoplasmic and microsomal proteins, but appeared to cause the greater change in proteins associated with the cytoplasm. The results indicate that most of the changes in protein expression associated with Al stress probably result from effects of Al on cell metabolism, and that only a few proteins may be induced or enhanced as part of a cultivar-specific mechanism of Al tolerance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1991.tb01505.x
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  • 2
    Electronic Resource
    Electronic Resource
    Protein Biomarkers of Phytotoxicity in Hazard Evaluation (1996)
    Springer
    Bulletin of environmental contamination and toxicology 56 (1996), S. 926 -934 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001289900134
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of various classes of herbicides on four species of algae
    Amsterdam : Elsevier
    Pesticide Biochemistry and Physiology 7 (1977), S. 203-209 
    Details
    ISSN: 0048-3575
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0048-3575(77)90011-6
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of amino acids on methionine-sulfoximine-induced heterocyst formation in Anabaena (1977)
    Springer
    Planta 136 (1977), S. 277-279 
    Details
    ISSN: 1432-2048
    Keywords: Amino acids ; Anabaena ; Glutamine synthetase ; Heterocysts ; Methionine sulfoximine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heterocyst development in ammonia-grown cultures of Anabaena variabilis and Anabaena 7120 was fully induced by the amino-acid analog methionine sulfoximine (MSO) at concentrations of 0.5–1.0 μM. Glutamine, glutamate, aspartate, and alanine at 0.5 mM blocked the induction of heterocysts by MSO in A. variabilis. With Anabaena 7120, glutamine and glutamate were fully effective and alanine partially effective in preventing MSO-induced heterocyst formation. In MSO-treated algae, glutamine synthetase activity was reduced to less than 15% of control values within 4–6 h. Inactivation of the enzyme was prevented by all four amino acids tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385996
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  • 5
    Electronic Resource
    Electronic Resource
    Transcriptional activity of heterocysts isolated from Anabaena variabilis (1987)
    Springer
    Archives of microbiology 148 (1987), S. 115-120 
    Details
    ISSN: 1432-072X
    Keywords: Heterocysts ; Anabaena ; Ribonucleic acid ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitrogenase activity, RNA synthesis, and protein synthesis were measured in heterocysts of Anabaena variabilis. Heterocysts labelled in situ for 4 h with [14C]uracil accumulated label in rRNA and tRNA to the same specific activity as RNA from vegetative cells. With isolated heterocysts, however, assimilation of [3H]uracil into RNa occurred at about 10% the rate in vegetative cells, and ceased 90 min after isolation. Pulse-chase experiments indicated that heterogeneous, high-molecular-weight RNA synthesized during the first 30 min of incubation was turned over during a 2 h chase, howver there was no accumulation of label in rRNA and tRNA as was seen with heterocysts labelled in situ and with vegetative cells. Assimilation of [3H]glycine into protein by isolated heterocysts was linear up to about 60 min, then proceeded at a slower rate for an additional 180 min. Maintenance of protein synsthesis and nitrogen fixation were both blocked by chloramphenicol and rifampicin. The data suggest that differentiated heterocysts continue to synthesize RNA and proteins and that these processes may contribute to the functional lifetime of heterocysts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425358
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  • 6
    Electronic Resource
    Electronic Resource
    Development of an ELISA for estimation of the copper nutritional status of wheat and cotton (1993)
    Springer
    Plant and soil 155-156 (1993), S. 453-456 
    Details
    ISSN: 1573-5036
    Keywords: copper deficiency ; cotton ; ELISA ; Gossypium hirsutum ; immunoassay ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Studies were carried out with hydroponically grown wheat and cotton to develop the Cu-requiring protein phenolase as a biomarker of Cu nutrient status. Isozymes of phenolase whose levels were reduced by Cu deficiency were identified by Western blots. A competitive enzyme-linked, immunosorbent assay (ELISA) was developed that could detect as little as 25 ng of phenolase. The ELISA revealed that Cu-sufficient cotton leaves had about 4-fold more phenolase antigen than did Cu-sufficient wheat leaves. In both species, the level of phenolase was reduced by 2- to 5-fold in leaves of Cu-deficient plants. Because the immunoassay for phenolase protein is rapid, inexpensive, and can be carried out with small amounts of leaf material, it has potential as a tool for assessment of the Cu status of crop plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025081
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  • 7
    Electronic Resource
    Electronic Resource
    Effect of low-dose ultrasonic treatment on growth rates and biomass yield of (1989)
    Springer
    Biotechnology techniques 3 (1989), S. 389-392 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Major project tasks included assembly of an ultrasonic treatment array and measurement of the cell culture growth rate as a function of ultrasonic frequency, and ultrasonic power level and dosage. Growth rates forAnabaena flos aquae were increased with both single or multiple ultrasonic dosages and were over and above that obtained with vigorous mechanical stirring.Selenastrum capricornutum growth rates were decreased by ultrasonic treatment. The results were also shown to be independent of the degree of cell agglomeration. Collectively, the data support the conclusion that low-dose, short duration ultrasonic treatment induces changes in culture growth rates in both algal species examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01875005
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