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  • 1
    Electronic Resource
    Electronic Resource
    382 Multihormonal potency of apudomas - an examplary case
    Amsterdam : Elsevier
    Journal of Steroid Biochemistry 19 (1983), S. 127 
    Details
    ISSN: 0022-4731
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0022-4731(83)91882-4
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Electron microscopic demonstration of calcitonin in human medullary carcinoma of thyroid by the immuno gold staining method (1984)
    Springer
    Histochemistry and cell biology 81 (1984), S. 369-372 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In a human medullary carcinoma of thyroid gland containing calcitonin in light microscopic demonstration by the avidin biotin complex (ABC) method characteristic secretory granules were found electron microscopically in the cytoplasm of the tumour cells. They consisted in so-called type I granules (270±25 nm) and type II granules (135±17 nm). By the immuno gold staining (IGS) method the content of many secretory granules measuring 85–270 nm (152±18 nm) in diameter could be identified as calcitonin. These granules seemed to be predominantly of type II because of their nearly corresponding size and feature. The type I grnaules were less frequent in number and they showed no or little immunoreactivity. The results indicate that the IGS-method is practicable to demonstrate the ultrastructural localization of calcitonin and to identify clearly the nature of intracytoplasmic granules in electron microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00514331
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A simple method for incubation of tissue sections in immunohistochemistry (1981)
    Springer
    Histochemistry and cell biology 72 (1981), S. 315-319 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The construction of a simple “incubatin chamber” is presented by which a homogeneous incubation of tissue sections with calculable amounts of immunoreagents is guaranteed. The incubation of large area sections, and parallel incubations of 10 slides are possible. As an example of application a case of alpha-1-antitrypsin deficiency in the liver is demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00517144
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    An alkaline-phosphatase staining method in avidin-biotin immunohistochemistry (1985)
    Springer
    Histochemistry and cell biology 82 (1985), S. 421-424 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An avidin-biotin alkaline-phosphatase (ABAP) staining method has been developed for the labeling of tissue sections and cell smears. The introduction of alkaline phosphatase as a marker enzyme through an avidin bridge results in excellent immunocytochemical labeling of different antigens using poly- and monoclonal antibodies. This technique avoids problems with endogenous peroxidase activity that sometimes occur using peroxidase staining procedures. The introduction of a preformed avidin-biotin alkaline-phosphatase complex (ABAPC) makes the presented technique as simple to handle as the widely used avidin biotin-peroxidase complex method (ABC). The ABAPC technique could be combined with other enzymatic labelings for double immunoenzymatic staining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02450475
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  • 5
    Electronic Resource
    Electronic Resource
    The use of monoclonal antibodies against thyroglobulin in immunoperoxidase techniques (1983)
    Springer
    Histochemistry and cell biology 78 (1983), S. 177-180 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A monoclonal mouse antibody to human thyroglobulin was used in a modified biotin-peroxidase-complex method to demonstrate thyroglobulin in different tissues and tumors of the thyroid gland. Mouse ascites fluid containing monoclonal antibodies could be diluted up to 1∶1,600 for detection of thyroglobulin, whereas no staining was observed in a medullary carcinoma. Nonspecific background staining was negligible. These results demonstrate that monoclonal antibodies against thyroglobulin are suitable in the immunohistochemical localisation of thyroid hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00489496
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    Paper (German National Licenses)
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