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  • 1
    Online Resource
    Online Resource
    Tokyo : United Nations University Press
    Keywords: Environmental protection International cooperation ; Oceanography Research ; Marine ecology ; Marine pollution Prevention
    Description / Table of Contents: The oceans cover more than 70 percent of the Earth's surface and play an important part in our lives by controlling climate and weather conditions; hosting shipping, transportation, recreation and tourism; and providing us with food, minerals and petroleum. The relationship between mankind and the oceans has been crucial since prehistoric times. With the growth of the human population, especially in coastal zones, there is a growing threat to oceans from land-based activities such as industrial effluent, municipal sewage, and runoff from agricultural areas, as well as antifouling agents used o
    Type of Medium: Online Resource
    Pages: Online-Ressource (xiii, 225 p) , ill., maps , 24 cm
    Edition: Online-Ausg. 2009 Electronic reproduction; Available via World Wide Web
    ISBN: 928081057X
    DDC: 333.91/64
    Language: English
    Note: Includes bibliographical references and index , CONTENTS; List of tables and figures; Preface; 1 Overview of the global marine and coastal challenges; Part I: Human activities related to marine life and management ; 2 Mankind belongs to the sea; 3 Environmental management of enclosed coastal seas; 4 International marine science activities in Japan; 5 The UNU's international marine environment researchnetworks: An approach towards sustainable seas in thetwenty-first century; Part II: Case studies of marine pollution in the world ; 6 Environmental problems in the coastal waters of China , 7 Marine pollution monitoring of butyltins and organochlorinesin the coastal waters of Thailand, the Philippines, and India8 Organochlorine contamination in Baikal seal (Phoca sibirica) fromLake Baikal, Russia; 9 Marine mammals and environmental contaminants in the PacificOcean: Current knowledge and frontiers for research; 10 Persistent organic pollutants and outbreaks of diseasein marine mammals; Part III: Marine biodiversity and environment in the Black Sea and the south-western Atlantic Ocean; 11 Biodiversity in the Black Sea: Threats and the future , 12 Marine biodiversity of the south-western Atlantic Oceanand main environmental problems of the regionGlossary; Acronyms; Contributors; Index , Electronic reproduction; Available via World Wide Web
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  • 2
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The taxonomic positions of Vibrio marinus and 11 related natural isolates were examined. Their phylogenetic positions on the basis of almost complete 16S rRNA sequences were determined by maximum likelihood, maximum parsimony and neighbor-joining analyses. V. marinus and the 11 isolates fell into a single cluster that was clearly distinct from other genera. There is now strong evidence that V. marinus should be reclassified as Moritella marina gen. nov., comb. nov.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 152 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The 16S rDNA genotypes among the family Vibrionaceae were determined using PCR/RFLP analysis. Five tetrameric restriction enzymes (Hha I, Dde I, Rsa I, Sau 3AI and Msp I) were used for RFLP analysis and adequate numbers of informative bands were obtained from each enzyme. Twenty-seven genotypes were obtained from 49 type and reference strains including 35 species. Nineteen species could be assigned to specific 16S rDNA genotypes, supporting the application of this analysis for identification. Trees constructed using five endonucleases resolved groups almost identical to those inferred from 16S rRNA gene sequencing. However, the branch lengths and detailed relationships among strains within a group differed from those inferred from sequence comparisons. The results of this study should be useful for genotyping, identification and approximate classification of natural isolates belonging to the family Vibrionaceae.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Marine biotechnology 1 (1999), S. 562-568 
    ISSN: 1436-2236
    Keywords: Key words: Archaeol, biomass measurement, methanogen, sediment, Tokyo Bay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract: An archaeal ether-linked lipid, archaeol, was determined to be a biomass indicator for methanogens both in the laboratory enriched culture and in marine sediments. The archaeol measurement method described by Ohtsubo et al. in 1993 was modified and applied to marine sediments. We compared the amount of archaeol with the cell number of methanogens or methane concentration in laboratory enriched culture of methanogens from marine sediment. Good correlations were obtained as follows: (Methane, mmol) = 11.2 × (Archaeol, mg): r= .996 or (Cell number) = 1.13 × 1011× (Archaeol, mg): r= .995, respectively. In the sediments of Tokyo Bay, archaeol was measured from approximately 46 to 561 ng/dry g sediment at the entrance to 267 to 4160 ng/dry g sediment at the innermost area. Using the coefficient from the laboratory experiment, these data corresponded to cell numbers of 5.2 × 106 to 4.7 × 108/dry g sediment. These values were 1 or 2 orders of magnitude higher than those obtained by culture methods in previous studies. Although dead or decomposed cells might be detected, archaeol measurement is useful for estimating the biomass of methanogens because of the good correlation between methane concentration and archaeol content in marine environments. In this study, we found a correlation of (Methane, mmol) = 0.012 × (Archaeol, mg): r= .932, n= 17 in marine sediments.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A deep ocean sampler (DOS) has been developed for microbiological sampling and is capable of aseptically collecting 400-ml water samples from any depth in the world oceans. The instrument maintains samples under in situ pressure and temperature. A hyperbaric transfer system has also been developed, enabling transfer of sample volumes up to 150 ml, without decompression or dilution, to pressurized incubation chambers. Utilization of14C-glutamate (21 to 96μg/l) and14C-acetate (4.6μg/l) by microbial populations in undecompressed water samples from the N.W. Atlantic and the Cape and Angola Basins was recorded over incubation periods of 2 to 18 weeks. Rates of substrate utilization ranged from 1 to 38×10−2 μg/l/day.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A significant number of viable colony-forming bacteria were recovered from deep-ocean bottom water samples passed through a 0.45μm filter. However, these bacteria small enough to pass through a 0.45μm membrane filter and termed “filterable bacteria” were less abundant in open-ocean surface water and coastal water samples. The reduced size of bacterial cells present in deep-ocean bottom water samples was documented by scanning electron microscopy. The concentration of ATP in the water samples was found to be correlated with results of direct counts of bacteria. Numerical taxonomy of bacterial strains isolated from water samples collected at two stations in the deep sea yielded taxonomic clusters grouped according to sample and size fraction. The generic composition of bacterial populations of bottom water filtrates was compared with that of bacteria retained by 0.45μ m filters. Strains ofAlcaligenes, Flavobacterium, Pseudomonas, andVibrio spp. were identified among those retained by, as well as passing through, 0.45μm filters. Two marine isolates obtained from the filtrate of a deep-ocean water sample were incubated for 9 weeks in nutrient-free artificial seawater, during which the cells became rounded and reduced in size. After the 9-week incubation period, more than 10% of the viable cells of both cultures were able to pass through a 0.4μm filter. The viable count at 9 weeks wasca. 10% of that of the initial population, although from direct counts the total population number remained relatively constant throughout the incubation period. From the observed reduction in cell size and increased starvation resistance of cells held under low nutrient conditions, it is concluded that a significant relationship exists between decreased cell size and increased survival of marine bacteria in the deep sea.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-868X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Barotolerant bacterium was isolated from sediment sample which was obtained from the depth of 4033 m in the Izu-Ogasawara Trench. The physiological property, growth characteristics and fatty acid composition were examined. The strain was a psychrotrophic and barotolerant bacterium, and was identified as species in the genusAlteromonas. The fatty acids of the strain were from C12 to C18. As the growth pressure increased, the portion of unsaturated fatty acid in membrane fraction increased due to an increase in the portion of C17∶1 and C18∶1, while the relative portion of C16∶0 and C16∶1 decreased. On the other hand, as the growth temperature decreased, the portion of unsaturated fatty acid increased due to the increase in the portion of C16∶1 and C18∶1.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-868X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Fluorescent oligonucleotide probes were used to detectVibrio cholerae directly under epifluorescence microscope. The probe which is complementary to the specific 16SrRNA sequence ofVibrio cholerae was labelled with Texas-Red, whereas the universal probe was labelled with Fluorescein. These probes allowed the distinctionVibrio cholerae from other eubacteria under the same microscopic field. In order to detect and enumerate specific bacteria in natural seawater, this method was combined with the direct viable count (DVC) technique. The combined method increased intracellular rRNA levels in the sample, and made it possible to detect the target bacteria with the specific gene probe. The applicability of this new method was confirmed both for the laboratory mixed culture system and natural seawater.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. A new approach to separate members of the genus Photobacterium from the genus Vibrio with RFLP (Restriction Fragment Length Polymorphism) patterns by HhaI digestion of PCR-amplified 16S rDNA was developed in the present study. It was clearly shown that these patterns of the genus Photobacterium were unique and distinguishable from Vibrio species. This method is very simple and does not need other supporting procedures, such as Southern transfer and probe hybridization. It can be applied not only to luminous species, but also to non-luminous Photobacterium spp. This result promises a rapid tool to distinguish the genus Photobacterium from Vibrio and should be useful in routine identification system.
    Type of Medium: Electronic Resource
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