ISSN:
1432-0886
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract In this paper we describe an analysis of the tissue distribution of two recently identified components of synaptonemal complexes (SCs), an Mr 125000 and an Mr 190000 protein, in the male rat by immunoblot analysis and immunocytochemical techniques. We compared the tissue distribution of these antigens with that of two earlier identified SC components, an Mr 30000 and an Mr 33000 polypeptide. For this purpose we used monoclonal antibodies (Mabs) that react exlusively with SCs in lysed spermatocytes, and that recognize the above mentioned antigens specifically in immunoblots of SC proteins or of nuclear proteins from spermatocytes: these were Mab IX9D5 (anti-190000), Mab IX5B2 (anti-125000), Mab II52F10 (anti-30000+33000), and Mab IX8G9 (anti-30000+33000). In the immunoblot experiments, we could detect the Mr 190000 and 125000 antigens exclusively in blots of SC proteins or nuclear proteins from spermatocytes; these antigens were not detectable in blots of nuclear proteins from liver, brain, spermatogonia or spermatids or in blots of proteins from mitotic chromosomes or nuclear laminae. With the anti-30000+33000 Mabs we obtained essentially the same result, except that Mab IX8G9, but not II52F10, recognizes a small amount of Mr 30000 antigen in blots of nuclear proteins from spermatids and spermatogonia. Although this might be ascribed to contamination of the isolated spermatids and spermatogonia, we cannot exclude that a small amount of Mr 30000 antigen is present in these cells. In the immunofluorescence analysis, the testis was the only tissue that reacted detectably with the above antibodies. Within the testis, spermatocytes and some early spermatids were the only cell types that contained detectable amounts of antigen. The Mr 125000 antigen was exclusively observed in nuclei of spermatocytes, from zygotene up to and including diplotene, in paired segments of SCs. The Mr 30000+33000 and 190000 antigens were present in paired as well as unpaired segments of SCs in nuclei of permatocytes, from zygotene up to and including diplotene and in the nuclei of some early spermatids in presumed remnants of SCs. We conclude that SCs consist largely of meiosisspecific proteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00357057
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