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  • 1
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    Mortality of wild golden grey mullet (Liza auratus) in Iranian waters of the Caspian Sea, associated with viral nervous necrosis-like agent (2018)
    Details
    Publikationsdatum: 2021-05-19
    Beschreibung: An acute mortality caused by an uncertain agent occurred in wild golden grey mullet (Liza auratus) population in the Iranian side of the Caspian Sea in February, 2004. Clinical signs of the moribund fish were erratic behavior such as spiral swimming, belly-up at rest and over inflation of swim bladder. Histological examination of brain of dead fish revealed hyperaemia, degeneration and necrosis in external granular layers as well as focal extension in intestine. Severe leucopenia was also observed. No putative causal factors were suggested by bacteriological, parasitological or environmental examinations. In virological examina-tions, however brains of affected golden grey mullet were positive in the reverse transcriptase-polymerase chain reaction test for piscine nodavirus (Nodaviridae, Betanodavirus), the causative agent of Encephalopathy and retinopathy (VER), otherwise known as viral nervous necrosis (VNN) in a variety of cultured marine fishes worldwide. Nucleotide sequence of the PCR amplicons is closely related with the coat protein gene of piscine nodaviruses, particularly redspotted grouper nervous necrosis virus (RGNNV). Although, it was not succeeded to isolate the virus, experimental infection with the brain homogenates of the affected fish in sevenband grouper (Epinephelus septemfasciatus), which is highly susceptible to RGNNV, produced neuro- logical abnormality followed by mortality. These results suggest that the mortality observed in golden grey mullet in the Caspian Sea might have been associated with piscine nodavirus infection.
    Beschreibung: Published
    Schlagwort(e): Golden grey mullet ; VER ; VNN ; Nodavirus ; PCR
    Repository-Name: AquaDocs
    Materialart: Journal Contribution , Refereed
    Format: pp.43-58
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    ARTIKEL (OA)
  • 2
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    Mortality of wild golden grey mullet (Liza auratus) in Iranian waters of the Caspian Sea, associated with viral nervous necrosis-like agent (2021)
    In:  http://aquaticcommons.org/id/eprint/22266 | 18721 | 2018-03-07 07:57:54 | 22266 | Iranian Fisheries Science Research Institute
    Details
    Publikationsdatum: 2021-07-01
    Beschreibung: An acute mortality caused by an uncertain agent occurred in wild golden grey mullet (Liza auratus) population in the Iranian side of the Caspian Sea in February, 2004. Clinical signs of the moribund fish were erratic behavior such as spiral swimming, belly-up at rest and over inflation of swim bladder. Histological examination of brain of dead fish revealed hyperaemia, degeneration and necrosis in external granular layers as well as focal extension in intestine. Severe leucopenia was also observed. No putative causal factors were suggested by bacteriological, parasitological or environmental examinations. In virological examinations, however brains of affected golden grey mullet were positive in the reverse transcriptase-polymerase chain reaction test for piscine nodavirus (Nodaviridae, Betanodavirus), the causative agent of Encephalopathy and retinopathy (VER), otherwise known as viral nervous necrosis (VNN) in a variety of cultured marine fishes worldwide. Nucleotide sequence of the PCR amplicons is closely related with the coat protein gene of piscine nodaviruses, particularly red spotted grouper nervous necrosis virus (RGNNV). Although, it was not succeeded to isolate the virus, experimental infection with the brain homogenates of the affected fish in sevenband grouper (Epinephelus septemfasciatus), which is highly susceptible to RGNNV, produced neurological abnormality followed by mortality. These results suggest that the mortality observed in golden grey mullet in the Caspian Sea might have been associated with piscine nodavirus infection.
    Schlagwort(e): Biology ; Fisheries ; Golden grey mullet ; VER ; VNN ; Nodavirus ; PCR ; Caspian Sea ; IRAN
    Repository-Name: AquaDocs
    Materialart: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 43-58
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
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    Normal-state spin dynamics in the iron-pnictide superconductors BaFe_{2}(As_{1−x}P_{x})_{2} and Ba(Fe_{1−x}Co_{x})_{2}As_{2} probed with NMR measurements (2013)
    American Physical Society (APS)
    Details
    Publikationsdatum: 2013-05-11
    Beschreibung: Author(s): Y. Nakai, T. Iye, S. Kitagawa, K. Ishida, S. Kasahara, T. Shibauchi, Y. Matsuda, H. Ikeda, and T. Terashima The NMR results in iron pnictides BaFe 2 (As 1− x P x ) 2 and Ba(Fe 1− x Co x ) 2 As 2 are analyzed based on the self-consistent renormalization (SCR) spin-fluctuation theory. The temperature dependence of the NMR relaxation rate T 1 −1 as well as the electrical resistivity is well reproduced by a SCR model where two... [Phys. Rev. B 87, 174507] Published Fri May 10, 2013
    Schlagwort(e): Superfluidity and superconductivity
    Print ISSN: 1098-0121
    Digitale ISSN: 1095-3795
    Thema: Physik
    Publiziert von American Physical Society (APS)
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  • 4
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    Diagnosis of coronary spastic angina by implantable loop recorder (2013)
    Oxford University Press
    In: Europace
    Details
    Publikationsdatum: 2013-04-27
    Print ISSN: 1099-5129
    Digitale ISSN: 1532-2092
    Thema: Medizin
    Publiziert von Oxford University Press
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  • 5
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    Enzymatic Formation of Intra-protein Thioether Bonds [Enzymology] (2015)
    The American Society for Biochemistry and Molecular Biology (ASBMB)
    Details
    Publikationsdatum: 2015-04-25
    Beschreibung: The bacterial enzyme designated QhpD belongs to the radical S-adenosyl-l-methionine (SAM) superfamily of enzymes and participates in the post-translational processing of quinohemoprotein amine dehydrogenase. QhpD is essential for the formation of intra-protein thioether bonds within the small subunit (maturated QhpC) of quinohemoprotein amine dehydrogenase. We overproduced QhpD from Paracoccus denitrificans as a stable complex with its substrate QhpC, carrying the 28-residue leader peptide that is essential for the complex formation. Absorption and electron paramagnetic resonance spectra together with the analyses of iron and sulfur contents suggested the presence of multiple (likely three) [4Fe-4S] clusters in the purified and reconstituted QhpD. In the presence of a reducing agent (sodium dithionite), QhpD catalyzed the multiple-turnover reaction of reductive cleavage of SAM into methionine and 5′-deoxyadenosine and also the single-turnover reaction of intra-protein sulfur-to-methylene carbon thioether bond formation in QhpC bound to QhpD, producing a multiknotted structure of the polypeptide chain. Homology modeling and mutagenic analysis revealed several conserved residues indispensable for both in vivo and in vitro activities of QhpD. Our findings uncover another challenging reaction catalyzed by a radical SAM enzyme acting on a ribosomally translated protein substrate.
    Print ISSN: 0021-9258
    Digitale ISSN: 1083-351X
    Thema: Biologie , Chemie und Pharmazie
    Publiziert von The American Society for Biochemistry and Molecular Biology (ASBMB)
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  • 6
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    A retrospective study of patients with follicular lymphoma (FL): identification of in situ FL or FL-like B cells of uncertain significance in lymph nodes resected at the time of previous surgery for carcinomas (2015)
    BMJ Publishing Group
    Details
    Publikationsdatum: 2015-06-20
    Beschreibung: Background and aims In situ follicular lymphoma (iFL)/intrafollicular neoplasia and follicular lymphoma (FL)-like B cells of uncertain significance represent proliferation of Bcl-2/t(14;18)-positive B cells solely in the germinal centres. The condition is interpreted as an early event in the multi-step lymphomagenesis of FL. The aim of this study is to examine the issue more specifically. Methods We reviewed medical history of FL patients in whom thoracoabdominal surgery with lymphadenectomy had been performed for management of carcinomas. These previously resected lymph nodes as well as the current FL nodes were analysed by immunohistochemistry, fluorescent in situ hybridisation, and PCR amplification with direct sequencing. Results We studied four such FL patients from a total of 150 patients with FL; all had iFL in the previously resected lymph nodes. Clonal relation was verified and suggested in one case each. The time from lymphadenectomy to the diagnosis of FL was 23–120 months. There appeared to be a reverse correlation between the rate of Bcl-2-positive follicle proliferation and the time from surgery to diagnosis of FL. Conclusions Although the rate for development of FL in individuals having iFL has been reported to be low from prospective studies, the present data indicate that follow-up studies for a longer period is necessary; the rate of Bcl-2-positive follicle proliferation could be a factor to predict development of FL in prospective studies. Such a retrospective study may contribute to elucidate mechanism(s) involved in lymphomagenesis of FL.
    Schlagwort(e): Immunology (including allergy)
    Print ISSN: 0021-9746
    Digitale ISSN: 1472-4146
    Thema: Medizin
    Publiziert von BMJ Publishing Group
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  • 7
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    Conformational Change of Topa Quinone in Copper Amine Oxidase [Enzymology] (2015)
    The American Society for Biochemistry and Molecular Biology (ASBMB)
    Details
    Publikationsdatum: 2015-09-19
    Beschreibung: The catalytic reaction of copper amine oxidase proceeds through a ping-pong mechanism comprising two half-reactions. In the initial half-reaction, the substrate amine reduces the Tyr-derived cofactor, topa quinone (TPQ), to an aminoresorcinol form (TPQamr) that is in equilibrium with a semiquinone radical (TPQsq) via an intramolecular electron transfer to the active-site copper. We have analyzed this reductive half-reaction in crystals of the copper amine oxidase from Arthrobacter globiformis. Anerobic soaking of the crystals with an amine substrate shifted the equilibrium toward TPQsq in an “on-copper” conformation, in which the 4-OH group ligated axially to the copper center, which was probably reduced to Cu(I). When the crystals were soaked with substrate in the presence of halide ions, which act as uncompetitive and noncompetitive inhibitors with respect to the amine substrate and dioxygen, respectively, the equilibrium in the crystals shifted toward the “off-copper” conformation of TPQamr. The halide ion was bound to the axial position of the copper center, thereby preventing TPQamr from adopting the on-copper conformation. Furthermore, transient kinetic analyses in the presence of viscogen (glycerol) revealed that only the rate constant in the step of TPQamr/TPQsq interconversion is markedly affected by the viscogen, which probably perturbs the conformational change. These findings unequivocally demonstrate that TPQ undergoes large conformational changes during the reductive half-reaction.
    Print ISSN: 0021-9258
    Digitale ISSN: 1083-351X
    Thema: Biologie , Chemie und Pharmazie
    Publiziert von The American Society for Biochemistry and Molecular Biology (ASBMB)
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  • 8
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    Cell Culture Isolation of Piscine Nodavirus (Betanodavirus) in Fish-Rearing Seawater [Methods] (2016)
    The American Society for Microbiology (ASM)
    Details
    Publikationsdatum: 2016-04-05
    Beschreibung: Piscine nodavirus (betanodavirus) is the causative agent of viral nervous necrosis (VNN) in a variety of cultured fish species, particularly marine fish. In the present study, we developed a sensitive method for cell culture isolation of the virus from seawater and applied the method to a spontaneous fish-rearing environment. The virus in seawater was concentrated by an iron-based flocculation method and subjected to isolation with E-11 cells. A real-time reverse transcriptase PCR (RT-PCR) assay was used to quantify the virus in water. After spiking into seawater was performed, a betanodavirus strain (redspotted grouper nervous necrosis virus [RGNNV] genotype) was effectively recovered in the E-11 cells at a detection limit of approximately 10 5 copies (equivalent to 10 2 50% tissue culture infective doses [TCID 50 ])/liter seawater. In an experimental infection of juvenile sevenband grouper ( Epinephelus septemfasciatus ) with the virus, the virus was isolated from the drainage of a fish-rearing tank when the virus level in water was at least approximately 10 5 copies/liter. The application of this method to sevenband grouper-rearing floating net pens, where VNN prevailed, resulted in the successful isolation of the virus from seawater. No differences were found in the partial sequences of the coat protein gene ( RNA2 ) between the clinical virus isolates of dead fish and the cell-cultured virus isolates from seawater, and the viruses were identified as RGNNV. The infection experiment showed that the virus isolates from seawater were virulent to sevenband grouper. These results showed direct evidence of the horizontal transmission of betanodavirus via rearing water in marine aquaculture.
    Print ISSN: 0099-2240
    Digitale ISSN: 1098-5336
    Thema: Biologie
    Publiziert von The American Society for Microbiology (ASM)
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  • 9
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    Evolutionary well-conserved region in the signal peptide of parathyroid hormone-related protein is critical for its dual localization through the regulation of ER translocation (2016)
    Oxford University Press
    Details
    Publikationsdatum: 2016-03-23
    Beschreibung: Parathyroid hormone-related protein (PTHrP) has two different targeting signals: an N-terminal signal peptide for the endoplasmic reticulum (ER) targeting and an internal nuclear localization signal. The protein not only functions as a secretory protein, but is also found in the nucleus and/or nucleolus under certain conditions. PTHrP signal peptide is less hydrophobic than most signal peptides mainly due to its evolutionarily well-conserved region (QQWS). The substitution of four tandem leucine residues for this conserved region resulted in a significant inhibition of the signal peptide cleavage. At the same time, proportion of nuclear and/or nucleolar localization decreased, probably due to tethering of the protein to the ER membrane by the uncleaved mutant signal peptide. Almost complete cleavage of the signal peptide accompanied by a lack of nuclear/nucleolar localization was achieved by combining the hydrophobic h-region and an optimized sequence of the cleavage site. In addition, mutational modifications of the distribution of charged residues in and around the signal peptide affect its cleavage and/or nuclear/nucleolar localization of the protein. These results indicate that the well-conserved region in the signal peptide plays an essential role in the dual localization of PTHrP through ER targeting and/or the membrane translocation.
    Print ISSN: 0021-924X
    Digitale ISSN: 1756-2651
    Thema: Biologie , Chemie und Pharmazie
    Publiziert von Oxford University Press im Namen von The Japanese Biochemical Society (JBS).
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  • 10
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    Novel Carbapenem Antibiotics for Parenteral and Oral Applications: In Vitro and In Vivo Activities of 2-Aryl Carbapenems and Their Pharmacokinetics in Laboratory Animals [Susceptibility] (2013)
    The American Society for Microbiology (ASM)
    Details
    Publikationsdatum: 2013-01-23
    Beschreibung: SM-295291 and SM-369926 are new parenteral 2-aryl carbapenems with strong activity against major causative pathogens of community-acquired infections such as methicillin-susceptible Staphylococcus aureus , Streptococcus pneumoniae (including penicillin-resistant strains), Streptococcus pyogenes , Enterococcus faecalis , Klebsiella pneumoniae , Moraxella catarrhalis , Haemophilus influenzae (including β-lactamase-negative ampicillin-resistant strains), and Neisseria gonorrhoeae (including ciprofloxacin-resistant strains), with MIC 90 s of ≤1 μg/ml. Unlike tebipenem (MIC 50 , 8 μg/ml), SM-295291 and SM-369926 had no activity against hospital pathogens such as Pseudomonas aeruginosa (MIC 50 , ≥128 μg/ml). The bactericidal activities of SM-295291 and SM-369926 against penicillin-resistant S. pneumoniae and β-lactamase-negative ampicillin-resistant H. influenzae were equal or superior to that of tebipenem and greater than that of cefditoren. The therapeutic efficacies of intravenous administrations of SM-295291 and SM-369926 against experimentally induced infections in mice caused by penicillin-resistant S. pneumoniae and β-lactamase-negative ampicillin-resistant H. influenzae were equal or superior to that of tebipenem and greater than that of cefditoren, respectively, reflecting their in vitro activities. SM-295291 and SM-369926 showed intravenous pharmacokinetics similar to those of meropenem in terms of half-life in monkeys (0.4 h) and were stable against human dehydropeptidase I. SM-368589 and SM-375769, which are medoxomil esters of SM-295291 and SM-369926, respectively, showed good oral bioavailability in rats, dogs, and monkeys (4.2 to 62.3%). Thus, 2-aryl carbapenems are promising candidates that show an ideal broad spectrum for the treatment of community-acquired infections, including infections caused by penicillin-resistant S. pneumoniae and β-lactamase-negative ampicillin-resistant H. influenzae , have low selective pressure on antipseudomonal carbapenem-resistant nosocomial pathogens, and allow parenteral, oral, and switch therapies.
    Print ISSN: 0066-4804
    Digitale ISSN: 1098-6596
    Thema: Biologie , Medizin
    Publiziert von The American Society for Microbiology (ASM)
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