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  • 1
    Electronic Resource
    Electronic Resource
    Identification of embryonal antigens of maize: Globulins as primary reserve proteins of the embryo (1978)
    Springer
    Planta 143 (1978), S. 11-20 
    Details
    ISSN: 1432-2048
    Keywords: Antigen spectra ; Embryo (proteins) ; γ-Plantlets ; Germination (seeds) ; Globulins ; Proteins (storage) ; Seedling growth ; Storage proteins ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Normal and γ-irradiated caryopses of Zea mays L. were germinated, and the degradation of embryonal antigens (EA) was followed in the endosperms, scutella and embryonic axes of the seedlings, using double immunodiffusion, immunoelectrophoresis and quantitative immunoprecipitation. The predominant transient EA were presumed to be storage proteins related to the reserve globulins of dicotyledonous seeds. Therefore globulins were isolated from maize scutella, purified by (NH4)2SO4 fractionation and isoelectric precipitation, and the molecular weights of the polypeptide units were estimated by discontinuous sodium-dodecyl-sulphate slab electrophoresis. The globulins were found to be identical with the predominant EA and amounted to about 40% of the protein nitrogen in the embryos of mature, non-germinated caryopses. The presumed reserve function of the globulins and the characteristic time course of their degradation in embryonic axes and scutella of maize seedlings are discussed in relation to the two-step pattern of mobilization of nitrogen reserves in germinating cereal caryopses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389046
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identical embryonal proteins in intact and isolated tissues of maize (Zea mays L.) (1979)
    Springer
    Planta 145 (1979), S. 245-251 
    Details
    ISSN: 1432-2048
    Keywords: Antigen ; Callus ; Cell suspension culture ; Dedifferentiation ; Meristem ; Proliferation proteins ; Proteins (in differentiation) ; Tissue culture ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Minor antigens characteristic of developing and mature embryos were not found in the shoot and root meristems of the seedlings. Some of these embryonal antigens (EA) were present, however, in callus and cell-suspension cultures, irrespective of their tissue origin, and were maintained throughout repeated subcultures, in some cases for more than 2 years. These EA were distinct both from the meristematic antigens found in the intact seedlings and in callus cultures, and from organ-specific antigens found only in intact plants. The EA of callus tissues derived from several maize genotypes were serologically identical. We therefore assume that these EA are “proliferation proteins” or “early proteins” expressed by cells that have not undergone any determination and lack any tissue or organ specificity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00454448
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Evidence for proteins specific for vascular elements in intact and cultured tissues and cells of maize (1980)
    Springer
    Planta 148 (1980), S. 116-123 
    Details
    ISSN: 1432-2048
    Keywords: Antigen ; Callus ; Cell suspension cultures ; Proteins (in differentiation) ; Seedling ; Vascular cylinder (stele) ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A group of antigenically distinct proteins characteristic for the tissue complex of the vascular cylinders was found in maize (Zea mays L.) seedlings using an immunofiltration technique. Specific stelar antigens present in the fully developed stele (vascular cylinder) of the primary root were also found in steles extracted from adventitious roots and from the mesocotyl but were absent, within the limits of sensitivity of the immunodiffusion tests employed, in root cortex and epidermis. Some of the stelar antigens were also evident in the meristem of the primary root and were present in traces in the scutellum, the mesocotyl node, and the primary leaves plus coleoptile. The specific stelar antigens could be traced in 13- and 15-day-old developing embryos and were definitely expressed by the 21 st day after pollination. Several stelar-specific antigens were found in embryo-derived callus tissues and in stem-derived cells maintained in serial suspension culture. Higher resolution of the stelar antigens by a modified technique of crossed immunoelectrophoresis was used to demonstrate several minor stelar antigens that were presumably characteristic exclusively of the completely differentiated stele. This technique along with sequential immunoprecipitation of labelled proteins provided a semiquantitative estimate of the specific stelar antigens in the meristem and the stele of the primary root, and in suspension-cultured cells which were devoid of noticeable signs of vascular differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386411
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  • 4
    Electronic Resource
    Electronic Resource
    Embryonal antigens in maize caryopses: The temporal order of antigen accumulation during embryogenesis (1977)
    Springer
    Planta 135 (1977), S. 225-231 
    Details
    ISSN: 1432-2048
    Keywords: Antigen accumulation ; Embryogenesis ; Globulins ; Proteins ; Seed proteins ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sequential appearance of a specific group of embryonal antigens (EA), presumably globulins, was demonstrated in developing maize (Zea mays L.) caryopses using a double immunodiffusion test with absorption of common antigens. Cross immunoelectrophoresis was employed to follow the differential pattern of EA accumulation in the growing scutellum and embryonic axis. The transient nature of two predominant EA seems to indicate their role as specific protein reserves of embryonal tissues. Another presumably organ-specific EA was maintained in callus obtained from a 28-day-old culture of scutellum isolated from the mature non-germinated caryopsis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384894
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    Paper (German National Licenses)
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