GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Document type
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    Antigenic determinants shared between HLA-A, -B, -C antigens and H-2 class I molecules modified by bovine beta-2 microglobulin (1988)
    Springer
    Immunogenetics 27 (1988), S. 158-158 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351095
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Antigenic determinants shared between HLA-A, −B, −C antigens and H-2 class I molecules modified by bovine beta-2 microglobulin (1987)
    Springer
    Immunogenetics 26 (1987), S. 351-355 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The specificity of the mouse class I-specific antibody COB6-3 was examined in detail. It was found to react with the mouse class I molecules H-2Db, Kd, and Qa-2, and with human HLA-A, −B, −C antigens. The specificity pattern of COB6-3, despite its different origin, was similar to that of the monomorphic HLA class I-specific antibody W6/32. Cross-inhibition studies show that on human cells the antigenic determinants recognized by the two antibodies are situated close together and may be identical. On mouse cells, reactivity of both antibodies was generated upon replacement of mouse beta-2 microglobulin (B2m) with its bovine counterpart, but differences in specificity were observed using human B2m.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00343703
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Serological and immunochemical analysis of H-2 class I molecules encoded by the D bregion (1984)
    Springer
    Immunogenetics 20 (1984), S. 341-345 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364215
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Intraspecies transfer via total cellular DNA of the gene for hypoxanthine phosphoribosyltransferase into cultured mouse cells (1979)
    Springer
    Molecular genetics and genomics 170 (1979), S. 179-185 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Under selective growth conditions a revertant of mouse cells, defective in hypoxanthine phosphoribosyltransferase activity (HPRT, EC-No. 2.4.2.8), was isolated, which contained an electrophoretically abnormal form of HPRT activity. The specific HPRT activity in crude extracts of the revertant cells is about 30% of the level determined in normal wild type cells. The variant HPRT reacts with antiserum against normal mouse HPRT but the rate of heat inactivation of the variant activity is different from the wild type form. By isozyme and karyotype analyses of somatic cell hybrids between the revertant mouse cells and Chinese hamster cells we found that the abnormal HPRT activity is coded for by the mouse X-chromosome as expected for a mutation in the structural HPRT gene. DNA has been purified from the abnormal HPRT revertant cells and incubated with mouse A9 cells (HPRT-). After growth in selective medium one clone was isolated which expressed the electrophoretically abnormal form of HPRT. Six clones showed the normal form of HPRT due to reversion of the defective HRRT locus in A9 cells. This result indicates DNA-mediated transfer of the mouse HPRT gene at a frequency of about 0.5×10-7. A similar frequency has been found for transfer of the variant HPRT locus via isolated metaphase chromosomes to A9 recipient cells. When placed in non-selective media the DNA-mediated transferent cells gradually lost their ability to express the HPRT transgenome at a rate of about 6% per average cell generation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337794
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Chromosomal gene transfer of human cytosol thymidine kinase into mouse cells (1978)
    Springer
    Molecular genetics and genomics 161 (1978), S. 49-57 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary If a chromosomal fragment transferred into recipient cells were integrated or strongly associated with a specific recipient chromosome it should segregate with this chromosome in hybrid cells. In order to corroborate this prediction we studied two independent mouse cell clones (“transferent clones”) which had taken up by chromosomal gene transfer a human chromosomal fragment carrying the gene for cytosol thymidine kinase (TKs, E.C.No.2.7.1.75). The following results were obtained. 1. Ten somatic cell hybrids isolated after fusion of transferent mouse clones and chinese hamster cells expressed functional human TKs and mouse galactokinase (GALK, E.C.No.2.7.1.6) activity. Counterselected derivatives of all clones had lost human TKs but still expressed mouse GALK. Recently both mouse genes for TKs and GALK have been assigned to mouse chromosome 11 (Kozak and Ruddle, 1977a, McBreen et al., 1977). Therefore our results argue against integration or association of the human gene for TKs at the site of the homologous defective mouse TKs-GALK-region in the genome of transferent clones. 2. In three somatic cell hybrids isolated after fusion of microcells from two different transferent mouse clones with established chinese hamster cells only human TKs but not mouse GALK was expressed. Karyotypic analysis of one hybrid suggested the presence of at least one copy of mouse chromosome 9 per hybrid cell. Chromosome 9 and the mouse isozyme activity of mannose phosphate isomerase which had been previously assigned to a gene locus on mouse chromosome 9 were missing in a subclone counter-selected against the presence of TKs activity. These findings suggested that the transferred human gene for TKs may be integrated or strongly associated with mouse chromosome 9 in this transferent mouse clone. 3. Two other somatic cell hybrids were analyzed which were derived from a different phenotypically stable transferent mouse clone by similar microcell fusion with the chinese hamster cells. Although these hybrids expressed human TKs activity, no mouse chromosome could be detected in these clones. Possibly after fusion of microcells derived from certain transferent mouse clones the transferred human chromosomal fragment could become translocated from a mouse chromosome to the chinese hamster genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266614
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...