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  • 1
    ISSN: 1438-2199
    Keywords: Amino acids ; Lysine ; Herbicides ; Azotobacter ; Xenobiotics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Production of lysine byAzotobacter chroococcum strain H23 was studied in chemically-defined media amended with different concentrations of alachlor, metolachlor, 2,4-D, 2,4,5-T and 2,3,6-TBA. The presence of 5, 10, and 50μg/ml of alachlor or 2,3,6-TBA significantly decreased quantitative production of lysine. However, the presence 2,4-D or 2,4,5-T at concentrations of 10 and 50μg/ml enhanced the production of lysine. Quantitative production of lysine was not affected as consequence of the addition of metolachlor to the culture medium, showing that the release lysine to the culture media byA. chroococcum was not affected by that herbicide.
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  • 2
    ISSN: 1432-0789
    Keywords: A. chroococcum ; Seed inoculation ; Zea mays ; Acetylene reduction assay ; Yield response ; Associative N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Inoculated and non-inoculated seedlings of Zea mays were grown in agricultural soils under aseptic and non-sterile conditions. Acetylene reduction activity and microbial counts were determined after 7 and 30 days of growth. Irrespective of the soil type Azotobacter spp. were commonly isolated under maize cultivation. Inoculation of agricultural soils with a suspension of A. chroococcum led to an increase in Azotobacter numbers, although this effect diminished with time. Nitrogenase activity was detected on maize roots and increased in response to the inoculation with A. chroococcum, showing that this associative growth could be of primary importance for the plant. The results of assays for acetylene reduction activity indicated that the nitrogenase activity was associated only with the root systems.
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  • 3
    ISSN: 1432-0789
    Keywords: A. chroococcum ; Zea mays ; Yield response ; Fertilizer N ; Inoculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field experiments were conducted during 1985 and 1986 to examine the effect of Azotobacter chroococcum on the grain yield of maize. Application of 40 kg N ha−1 plus A. chroococcum caused a significant increase in maize yield. Azotobacter inoculation was more efficient at lower doses (40 kg N ha−1) than at high doses (80 kg N ha−1) of urea.
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  • 4
    ISSN: 1432-0789
    Keywords: Key words Azotobacter ; Azospirillum ; Rhizobium ; Vicia faba L. ; Legume inoculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Mixed inoculation of Vicia faba L. with four different Rhizobium/Azospirillum and Rhizobium/Azotobacter combinations led to changes in total content, concentration and/or distribution of the mineral macro- and micronutrients, K, P, Ca, Mg, Fe, B, Mn, Zn and Cu, when compared with plants inoculated with Rhizobium only. The effects varied to a great extent among the Azotobacter and Azospirillum strains selected for combined inoculation.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 13 (1992), S. 173-175 
    ISSN: 1432-0789
    Keywords: Methidathion ; Soil microflora ; Enumeration of functional bacterial groups ; Acetylene reduction assay ; ARA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We studied the effects of the organophosphorus insecticide methidathion, at concentrations of 10, 50, 100, 200 and 300 μg g-1 in an agricultural soil, on fungi, total bacterial populations, aerobic N2-fixing bacteria, denitrifying bacteria, nitrifying bacteria (phases I and II), and nitrogenase activity (acetylene reduction assay). The presence of 10–300 μg g-1 of methidathion significantly increased fungal populations (colony-forming units). Denitrifying bacteria, aerobic N2-fixing bacteria and N2 fixation were significantly increased at concentrations of 50–300 μg g-1. The total number of bacteria increased significantly at concentrations of 100–300 μg g-1. Nitrifying bacteria decreased initially at concentrations of 300 μg g-1, but recovered rapidly to levels similar to those in the control soil without the insecticide.
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  • 6
    ISSN: 1432-072X
    Keywords: Azotobacter ; Low-phosphate medium-Adenine nucleotides ; Energy charge-Poly ; β-hydroxybutyrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of a low phosphate concentration on intracellular adenine nucleotide content, oxygen consumption and poly-β-hydroxybutyrate deposition was investigated with N-free and NH 4 + batch cultures of Azotobacter vinelandii. When the microorganisms were cultured under low-phosphate concentrations the cells contained much larger amounts of poly-β-hydroxybutyrate, but displayed lower oxygen consumption activities and energy charge values than did control cells. Also, the ratio ATP to ADP was much higher in control cells and the intracellular levels of ATP were lower in low-phosphate cells.
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  • 7
    ISSN: 1432-072X
    Keywords: Azotobacter vinelandii ; Phenolic acids ; Nitrogenase activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth and nitrogenase activity (acetylene reduction) of Azotobacter vinelandii in chemically defined N-free media were studied in the presence of p-hydroxybenzoic, vanillic, p-coumaric, and ferulic acids at concentrations from 0.01 to 1% (w/v). Growth and nitrogenase activity were only detected when the microorganism was cultured on p-hydroxybenzoic acid either as sole carbon source or mixed with other phenolic acids, suggesting that p-hydroxybenzoic acid could be utilized as a carbon source by A. vinelandii for growing under certain environmental conditions.
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  • 8
    ISSN: 1438-2199
    Keywords: Amino acids ; Azotobacter ; Azospirillum ; Biotechnology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Interest of microbial production of amino acids has been increased greatly since development of biotechnological methods. These methods represent a perspective way applied in a future large-scale manufacture of inexpensive amino acids. In this context, the isolation of producing organisms that may be exploited in the desing of alternative methods for the production of amino acids could be of primary importance. In this review we will describe the liberation of amino acids (methionine, lysine, arginine, tryptophane and glutamic acid) byAzotobacter andAzospirillum during growth in culture media with different carbon sources under diazotrophic and adiazotrophic conditions. These organisms may be useful in developing new methods for the industrial production of amino acids.
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  • 9
    ISSN: 1573-3017
    Keywords: Alachlor ; herbicides ; pesticides ; soil bacteria ; enzymatic activities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: A study was made of the effects of one selected acetanilide herbicide, alachlor, at concentrations of 2.0–10.0 kg ha−1 on bacterial populations, fungi, dinitrogen fixation bacteria, denitrifying bacteria, nitrifying bacteria, nitrogenase activity, acid and alkaline phosphatases, arylsulfatase and deshydrogenase. The presence of 2.0–10.0 kg ha−1 of alachlor in the soil increased the total number of bacteria and fungi. The population of denitrifying bacteria increased significantly at concentrations of 5.0–10.0 kg ha−1. However, aerobic dinitrogen fixing bacteria and nitrogenase activity decreased at alachlor concentrations of 3.5–10.0 kg ha−1. Acid and alkaline phosphatases, arylsulfatase and dehydrogenase activity decreased significantly initially at concentrations of 5.0–10.0 kg ha−1, but recovered to levels similar to those in the control. Nitrifying bacteria were not affected as a consequence of the addition of the herbicide to agricultural soil.
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  • 10
    ISSN: 1573-5036
    Keywords: chlorpyrifos ; insecticides ; methylpyrimifos ; organophosphorus ; soil microflora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A study of the effects of two selected organophosphorus insecticides methylpyrimifos and chlorpyrifos on soil microflora in an agricultural loam was made. The insecticides had concentrations of 10 to 300 μg g−1. The presence of methylpyrimifos at concentrations of 100 to 300 μg g−1 or chlorpyrifos at concentrations from 10 to 300 μg g−1 significantly decreased aerobic dinitrogen fixing bacteria and dinitrogen fixation. Nitrifying bacteria decreased at concentrations of 200 and 300 μg g−1 of methylpyrimifos. The presence of 10 to 300 μg g−1 of chlorpyrifos decreased the total number of bacteria. However, fungal populations and denitrifying bacteria were not affected as a consequence of the addition of the organophosphorus insecticides to the agricultural soil, showing that these microorganisms can tolerate high amounts of those insecticides.
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