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  • 1
    Electronic Resource
    Electronic Resource
    The Use of Protamine-Coated Slides for Immobilizing Protozoa (1973)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 20 (1973), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Fresh-water, marine or parasitic protozoa can be immobilized for microscopic observation on protaminecoated slides. They appear not to be harmed by this procedure. The type of protamine derivative and its concentration are important, best results being obtained with 0.015–0.05% (w/v) of protamine sulfate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1973.tb06010.x
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  • 2
    Electronic Resource
    Electronic Resource
    Growth kinetics and nitrogen-nutrition of the marine diatom Phaeodactylum tricornutum in continuous dialysis culture (1991)
    Springer
    Journal of applied phycology 3 (1991), S. 1-10 
    Details
    ISSN: 1573-5176
    Keywords: dialysis culture ; continuous growth ; nitrogen metabolism ; diatoms ; Phaeodactylum tricornutum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth kinetics and nitrogen (N)-nutrition of the marine pennate diatom Phaeodactylum tricornutum Bohlin were determined in continuous dialysis culture at different cell densities. Inflow nutrient medium was supplied as natural unenriched estuarine seawater to a dialysis culture system with a high ratio of membrane surface area/culture volume (Am/Vc). Under the experimental conditions, the supply of inorganic macronutrients (NO 3 − + NO 4 − and PO 4 −3 ) by diffusion (Nd) was markedly greater than that provided by the dilution (FfCN) of the culture (Nd ≫ FfCN), thereby establishing an inverse relationship between the cell density and the dilution rate (D). This continuous dialysis system allows for the maintenance of prolonged growth (〉 two weeks) at various cell densities (1.4 to 27.2 × 109 cells 1−1) within a range of dilution rates between 0.30 to 1.08 d−1. In high cell density cultures, where the extracellular medium was characterized as nutrient deficient, a lower growth rate (μe) was exhibited than in cultures with lower cell densities. The growth rate (μe) remained equivalent to the dilution rate (D) throughout the culture cycle, indicating that equilibrated growth was achieved. High cell density cultures yielded higher productivity (P), relative to that of cultures grown at lower cell densities, in terms of cell-N and −C produced per unit time. However, cell quotas of both N and C declined with increasing cell concentrations. Denser cultures were characterized by an enhanced N-conversion efficiency (YN) and a higher cellular N/C atomic ratio. The nutritional response of this diatom in dense cultures reveals an efficient use of N-nutrients, presumably as a result of cellular nutrient adaptation to oligotrophic conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00003914
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  • 3
    Electronic Resource
    Electronic Resource
    Un nouveau procede de culture continue a dialyse pour le phytoplancton (1981)
    Springer
    Biotechnology letters 3 (1981), S. 689-694 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Equipment and methods for continuous dialysis culture are described which make feasible the continous aseptic production of dense cultures of phytoplankton (unicellular marine algae). New features include the use of independently-replaceable fibre dialysis units and renewal of the nutrient medium (sea water) by controlled hydraulic diffusion. Cell densities of up to 4 × 107 are attained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00134845
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  • 4
    Electronic Resource
    Electronic Resource
    Biocatalysis of chlorophyllase from Phaeodactylum tricornutum in a biphasic organic system (1994)
    Chichester : Wiley-Blackwell
    Journal of Chemical Technology AND Biotechnology 60 (1994), S. 73-81 
    Details
    ISSN: 0268-2575
    Keywords: chlorophyllase ; Phaeodactylum tricornutum ; biocatalysis ; biphasic organic solvent ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The hydrolytic activity of a partially purified chlorophyllase, obtained from the alga Phaeodactylum tricornutum, was determined in a biphasic organic system using different mixtures of hexane and Tris-HCl buffer solution (20 mmol dm-3, pH 7·5). The most appropriate kinetic parameters were determined for the optimum hydrolytic activity of chlorophyllase including hexane concentration (45%), optimum pH (7·5), time of incubation (7 h), incubation temperature (25°C) and enzyme concentration (10 μg cm-3). The optimum concentrations of magnesium chloride and dithiothreitol, used as activators, were determined to be 4 mmol dm-3 and 5 mmol dm-3, respectively. The results also indicated that diisopropyl fluorophosphate (DIFP) had a mixed competitive-non-competitive inhibitory effect on chlorophyllase activity. The end-products of the chlorophyllase hydrolytic reaction were identified and confirmed, using thin-layer and high-performance liquid chromatographies, spectrophotometric scanning and Fourier transform infrared spectroscopy.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jctb.280600112
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