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  • 1
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    Fluorescence properties of dissolved organic matter from Leverett Glacier outflow (2020)
    PANGAEA
    Details
    Publication Date: 2023-10-28
    Description: The outflow of Leverett Glacier, a large land-terminating glacier of the Greenland Ice Sheet was sampled over the 2015 ablation season. Dissolved organic carbon (DOC) concentrations, dissolved organic matter (DOM) fluorescence, and hydrogeochemical data (e.g. discharge, specific conductivity, pH, and turbidity) were analyzed to assess changing DOM sources over the melt season. DOC concentrations and red-shifted fluorescence suggest terrestrial inputs from overridden soils dominated DOM early season inputs before progressive dilution with increasing discharge. During the outburst period, supraglacial drainage events disrupted the subglacial drainage system and introduced dominant protein-like fluorescence signatures not observed in basal flow. These results suggest that subglacial hydrology and changing water sources influence exported DOC concentration and DOM composition, differentiated through fluorescence characteristics. The outburst and post-outburst periods were characterized by protein-like fluorescence from supraglacial and potentially subglacial microbial sources.
    Keywords: Campbell Scientific 247-L Conductivity and Temperature Probe; Carbon, organic, dissolved; Carbon, organic, dissolved load; Conductivity; DATE/TIME; Day of the year; Dissolved Organic Matter; DOM; Event label; Flow rate; Fluorescence; Fluorescence, dissolved organic matter, at 317 nm wavelength; Fluorescence, dissolved organic matter, at 368 nm wavelength; Fluorescence, dissolved organic matter, at 416 nm wavelength; Fluorescence, dissolved organic matter, at 425 nm wavelength; Fluorescence, dissolved organic matter, at 483 nm wavelength; Fluorescence spectrometer, AquaLog, HORIBA JobinYvon; glaciers; Greenland; Honeywell Durafet pH sensors; Lachat QuickChem 8500 flow injection autoanalyser; L Atalante; Leverett_glacier_basal_ice; Leverett_glacier_snow; Leverett_glacier_time_series; Nitrogen in nitrate; PARAFAC; Period; pH; Phosphorus in orthophosphate; Pressure transducer, HOBO; Roorkee Industries Suspended Sediment Sampler USDH-48; Shimadzu TOC-L total organic carbon analyzer; SNOW; Snow/ice sample; Suspended particulate matter; Thermo Scientific Dionex IonPac AS11-HC-4μm Anion-Exchange Column fitted to a Thermo Scientific Dionex ICS-5000 Ion Chromatography; ZAIROV
    Type: Dataset
    Format: text/tab-separated-values, 785 data points
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    DATA PANGAEA
  • 2
    Electronic Resource
    Electronic Resource
    An unusual right lung (1918)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 15 (1918), S. 101-102 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1090150206
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    Paper (German National Licenses)
    Fulltext
  • 3
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    Marine aerosol source regions to Prince of Wales Icefield, Ellesmere Island, and influence from the tropical Pacific, 1979–2001 (2016)
    Wiley-Blackwell
    Details
    Publication Date: 2016-08-06
    Description: Using a coastal ice core collected from Prince of Wales (POW) Icefield on Ellesmere Island, we investigate source regions of sea ice-modulated chemical species (methanesulfonic acid (MSA) and chloride (Cl - )) to POW Icefield, and the influence of large-scale atmospheric variability on the transport of these marine aerosols (1979–2001). Our key findings are: (1) MSA in the POW Icefield core is derived primarily from productivity in the sea-ice zone of Baffin Bay and the Labrador Sea, with influence from waters within the North Water (NOW) polynya, (2) sea-ice formation processes within the NOW polynya may be a significant source of sea-salt aerosols to the POW core site, in addition to offshore open water source regions primarily in Hudson Bay, and (3) the tropical Pacific influences the source and transport of marine aerosols to POW Icefield through its remote control on regional winds and sea-ice variability. Regression analyses during times of MSA deposition reveal sea level pressure (SLP) anomalies favorable for opening of the NOW polynya, and subsequent oceanic dimethyl sulfide production. Regression analyses during times of Cl - deposition reveal SLP anomalies that indicate a broader oceanic region of sea-salt sources to the core site. These results are supported by SMMR- and SSM/I-based sea-ice reconstructions and air mass transport density analyses, and suggest the marine biogenic record may capture local polynya variability, while sea-salt transport to the site from larger offshore source regions in Baffin Bay is likely. Regression analyses show a link to tropical dynamics via an atmospheric Rossby wave.
    Print ISSN: 0148-0227
    Topics: Geosciences , Physics
    Published by Wiley-Blackwell on behalf of American Geophysical Union (AGU).
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    PAPER CURRENT
  • 4
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    The role of conserved residues in Fdc decarboxylase in prenylated flavin mononucleotide oxidative maturation, cofactor isomerization, and catalysis [Protein Structure and Folding] (2018)
    The American Society for Biochemistry and Molecular Biology (ASBMB)
    Details
    Publication Date: 2018-02-17
    Description: The UbiD family of reversible decarboxylases act on aromatic, heteroaromatic, and unsaturated aliphatic acids and utilize a prenylated flavin mononucleotide (prFMN) as cofactor, bound adjacent to a conserved Glu–Arg–Glu/Asp ionic network in the enzyme's active site. It is proposed that UbiD activation requires oxidative maturation of the cofactor, for which two distinct isomers, prFMNketimine and prFMNiminium, have been observed. It also has been suggested that only the prFMNiminium form is relevant to catalysis, which requires transient cycloaddition between substrate and cofactor. Using Aspergillus niger Fdc1 as a model system, we reveal that isomerization of prFMNiminium to prFMNketimine is a light-dependent process that is largely independent of the Glu277–Arg173–Glu282 network and accompanied by irreversible loss of activity. On the other hand, efficient catalysis was highly dependent on an intact Glu–Arg–Glu network, as only Glu → Asp substitutions retain activity. Surprisingly, oxidative maturation to form the prFMNiminium species is severely affected only for the R173A variant. In summary, the unusual irreversible isomerization of prFMN is light-dependent and probably proceeds via high-energy intermediates but is independent of the Glu–Arg–Glu network. Our results from mutagenesis, crystallographic, spectroscopic, and kinetic experiments indicate a clear role for the Glu–Arg–Glu network in both catalysis and oxidative maturation.
    Print ISSN: 0021-9258
    Electronic ISSN: 1083-351X
    Topics: Biology , Chemistry and Pharmacology
    Published by The American Society for Biochemistry and Molecular Biology (ASBMB)
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    PAPER CURRENT
  • 5
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    S-nitrosylation of Ras in breast cancer (2012)
    BioMed Central
    Details
    Publication Date: 2012-11-16
    Description: Elevated expression of nitric oxide synthase 2 (NOS2) has been recently shown to correlate with poor survival in estrogen receptor-negative breast cancer. In an article in Breast Cancer Research, Switzer and colleagues identify the transcription factor Ets-1 as a critical mediator of nitric oxide-dependent oncogenic gene expression in basal-like breast cancer. This pathway is driven by S-nitrosylation of wild-type Ras, which leads to MAP kinase-dependent phosphorylation and activation of Ets-1. These results establish a new role for S-nitrosylation in mediating an aggressive breast cancer phenotype.
    Print ISSN: 1465-5411
    Electronic ISSN: 1465-542X
    Topics: Medicine
    Published by BioMed Central
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    PAPER CURRENT
  • 6
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    Agriculture, Vol. 5, Pages 353-366: Variation in Response to Moisture Stress of Young Plants of Interspecific Hybrids between White Clover (T. repens L.) and Caucasian Clover (T. ambiguum M. Bieb.) (2015)
    MDPI
    Details
    Publication Date: 2015-06-20
    Description: Backcross hybrids between the important forage legume white clover (Trifolium repens L.), which is stoloniferous, and the related rhizomatous species Caucasian clover (T. ambiguum M. Bieb), have been produced using white clover as the recurrent parent. The effect of drought on the parental species and two generations of backcrosses were studied in a short-term glasshouse experiment under three intensities of drought. Plants of Caucasian clover maintained a higher leaf relative water content and leaf water potential than white clover at comparable levels of drought, with the response of the backcrosses generally intermediate between the parents. Severe drought significantly reduced stolon growth rate and leaf development rate of white clover compared to the control, well-watered treatment, whilst differences between these two treatments in the backcross hybrids were relatively small.   The differences between parental species and the backcrosses in root morphology were studied in 1m long vertical pipes. The parental species differed in root weight distribution, with root weight of Caucasian clover significantly greater than white clover in the 0.1 m to 0.5 m root zone. The backcrosses exhibited root characteristics intermediate between the parents. The extent to which these differences influence the capacity to tolerate drought is discussed.
    Electronic ISSN: 2077-0472
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by MDPI
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  • 7
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    Automated Software System to Promote Anticoagulation and Reduce Stroke Risk [Brief Report] (2017)
    American Heart Association (AHA)
    In: Stroke
    Details
    Publication Date: 2017-02-28
    Description: Background and Purpose—Oral anticoagulants (OAC) substantially reduce risk of stroke in atrial fibrillation, but uptake is suboptimal. Electronic health records enable automated identification of people at risk but not receiving treatment. We investigated the effectiveness of a software tool (AURAS-AF [Automated Risk Assessment for Stroke in Atrial Fibrillation]) designed to identify such individuals during routine care through a cluster-randomized trial.Methods—Screen reminders appeared each time the electronic health records of an eligible patient was accessed until a decision had been taken over OAC treatment. Where OAC was not started, clinicians were prompted to indicate a reason. Control practices continued usual care. The primary outcome was the proportion of eligible individuals receiving OAC at 6 months. Secondary outcomes included rates of cardiovascular events and reports of adverse effects of the software on clinical decision-making.Results—Forty-seven practices were randomized. The mean proportion–prescribed OAC at 6 months was 66.3% (SD=9.3) in the intervention arm and 63.9% (9.5) in the control arm (adjusted difference 1.21% [95% confidence interval −0.72 to 3.13]). Incidence of recorded transient ischemic attack was higher in the intervention practices (median 10.0 versus 2.3 per 1000 patients with atrial fibrillation; P=0.027), but at 12 months, we found a lower incidence of both all cause stroke (P=0.06) and hemorrhage (P=0.054). No adverse effects of the software were reported.Conclusions—No significant change in OAC prescribing occurred. A greater rate of diagnosis of transient ischemic attack (possibly because of improved detection or overdiagnosis) was associated with a reduction (of borderline significance) in stroke and hemorrhage over 12 months.Clinical Trial Registration—URL: http://www.isrctn.com. Unique Identifier: ISRCTN55722437.
    Keywords: Atrial Fibrillation, Anticoagulants, Health Services, Ischemic Stroke
    Print ISSN: 0039-2499
    Electronic ISSN: 1524-4628
    Topics: Medicine
    Published by American Heart Association (AHA)
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    PAPER CURRENT
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