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  • 1
    Keywords: Microbiology Congresses ; Cell aggregation Congresses ; Cell adhesion Congresses ; Konferenzschrift ; Konferenzschrift 1984 ; Mikroorganismus ; Zelladhäsion
    Type of Medium: Book
    Pages: X, 423 S. , Ill., graph. Darst. , 8°
    ISBN: 3540139966 , 0387139966
    Series Statement: Life sciences research report 31
    DDC: 576.11
    RVK:
    RVK:
    Language: English
    Note: Literaturangaben
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 8 (1991), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 15 (1994), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Gene transfer among microorganisms has been well demonstrated in laboratory microcosms and in situ, under non-limiting nutrient conditions. The literature contains conflicting opinions, however, as to whether such processes could occur in the absence of nutrients. This review summarises the evidence for the occurrence of gene transfer by conjugation, transformation and transduction among non-growing bacteria in nutrient depleted environments. Conjugation by selftransmissible, or by non-selftransmissible but mobilisable, plasmids has been shown to occur among environmental isolates of Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa and marine Vibrio strains. Transduction and transformation have been demonstrated in isolates of P. aeruginosa and marine Vibrio strains, respectively. It is possible that the mechanisms of these processes may be different in non-growing cells in nutrient depleted conditions, compared to those occurring in cells growing in rich media.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The previously described pLOFKm transposon delivery plasmid (J. Bacteriol. (1990) 172, 6557–6567) was engineered such that a promoterless lacZ gene was cloned within the transposon cassette, generating the vector pLBT. Using pLBT, stable insertion mutations were generated at high frequencies in Vibrio sp. S141 and Pseudomonas sp. S91, and the interrupted genes could be monitored for their pattern of regulation. Genetic screens isolated mutants defective in a variety of activities. We describe the construction and use of pLBT as a tool for reporter gene mutant analysis in bacteria other than well-characterized laboratory strains.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 140 (1996), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Characterisation of two genes in Pseudomonas sp. S91 that are responsive to carbon dioxide is reported. These were identified by random transposon mutagenesis leading to fusion of the Escherichia coli lacZ reporter gene to the genes of interest. Expression of the genes' promoters was quantified by measuring the reporter gene product, β-galactosidase. β-Galactosidase synthesis was induced when cells were exposed to 10% CO2 on solid media or during growth in aqueous phase when the culture density was greater than 1 at 610 nm, in either rich or minimal media. Induction of β-galactosidase synthesis was not due to: increased alkalinity, onset of stationary phase, build up of soluble metabolites in the culture supernatant, or cell density-dependent signalling. The CO2-inducible gene fusions were not induced by other environmental conditions that are known to stimulate global regulators of environmental gene expression. Benzole acid (2 mM) induced β-galactosidase synthesis in one of the mutants indicating the CO2 response may involve the intracellular CO2 partial pressure/bicarbonate ion concentration/pH equilibrium.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 85 (1991), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The basis for this work was to investigate whether bacteria immobilized on or in a solid substrate resulted in the release of low molecular weight products that act as chemoattractants for other bacteria In the surrounding aqueous phase. Chemotaxis of a marine vibrio was observed microscopically along the gradient of breakdown products generated by a Flavobacterium sp. immobilized in an alginate-agar bead. The chemotactic phenomenon was confirmed by a viable counting technique. The chemoattractant was derived from neither the alginate nor the agar but probably was amino acids produced from protein present as a contaminant in the alginate.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 31 (1985), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The initial selective adhesion of bacteria, expressing growth on solid media with low, intermediate, and high nutrient concentrations, to immersed glass surfaces in seawater was examined. Copiotrophic-type bacteria grown on high nutrient medium did not show a competitive advantage as primary colonizers. As compared to bacterial numbers in bulk water, relatively higher numbers of adhered oligotrophic-type bacteria, exhibiting growth on low-nutrient media, were found during the initial phase of adhesion. Higher numbers of copiotrophic rather than oligotrophic-type bacteria were seen in the bulk water. The majority of the adherent bacteria was irreversibly bound. Characteristics such as cell size, degree of cell surface hydrophobicity, and motility of bacterial isolates from the different nutrient concentrations did not account for the observed, possibly selective, adhesion. Although bacteria expressed nutritionally different requirements and adaptations at the time of sampling, successive reinoculations of a total of 161 isolates essentially failed to show the existence of obligacy of any given nutritional type of bacteria. The expression of different nutritional adaptations of bacteria in low-nutrient marine waters was also suggested by showing the inability of oligotrophic-like bacteria to possess starvation survival mechanisms such as those displayed by copiotrophs [3].
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 4 (1989), S. 181-187 
    ISSN: 1476-5535
    Keywords: Nocardia amarae ; Surface tension ; Hydrocarbon affinity ; Montmorillonite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Cultures ofNocardia amarae give rise to cell-stabilized foams in a laboratory scale foaming apparatus. The organism produces a surfactant and the cells are very hydrophobic; factors which, in terms of froth flotation theory, are essential for foam production and transport of the cells from the aqueous to the bubble phase. The addition of montmorillonitic clay to the culture prior to foaming prevents foam stabilization. The results obtained suggest the formation of a salt-dependent, reversible, bacterium-montmorillonite complex which prevents transport of cells to the bubble phase.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 138 (1984), S. 84-88 
    ISSN: 1432-072X
    Keywords: Leptospira adhesion ; Solid-liquid interface ; Dynamic model ; Motility ; Reversible ; Irreversible
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two strains of the saprophytic Leptospira biflexa serovar patoc display reversible and irreversible adhesion at a solid-liquid interface. Both forms of adhesion are enhanced in the presence of 20 μM carbonyl cyanide metachlorophenyl hydrazone (CCCP), an uncoupler which inhibits motility of the bacteria. Microscopic observations also indicated that motility may have a role in adhesion as only actively motile organisms were seen to detach from the substratum. A dynamic model is proposed for adhesion of these organisms at a solid-liquid interface. It is suggested that the level of reversible adhesion is determined by the comparative rates of attachment (ON phase) and detachment (OFF phase). As reversible adhesion is mediated by weak forces of attraction, bacterial motility or gentle washing could promote the OFF phase. When motility is inhibited, the OFF phase is reduced and the ON phase continues (as motility is not required for the ON phase) causing the level of reversible adhesion to increase. Since reversible adhesion is a prerequisite for irreversible adhesion, then increased reversible adhesion leads directly to increased irreversible adhesion. Reversible adhesion appears to be mediated by the weak attractive forces of the “secondary minimum” whereas the mechanism facilitating irreversible adhesion of leptospires is not known.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 153 (1990), S. 175-180 
    ISSN: 1432-072X
    Keywords: Respiration ; Respiratory Na+ pump ; Survival ; Dwarfing ; Vibrios
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The marine bacterium Vibrio fluvialis NCTC11328 responded to nutrient depletion by a reduction in cell volume, and this was prevented by conditions that eliminated respiration as a source of energy. Addition of the protonophore, CCCP, removal of oxygen and introduction of mutations leading to defects of the respiratory chain prevented size reduction during periods of nutrient limitation. Further, survival of the wild-type strain during starvation was reduced under anaerobic conditions and survival of respiratory mutants under aerobic conditions was reduced compared with that of the parent strain. Removal by mutation of the respiratory Na+ pump from Vibrio alginolyticus did not inhibit size reduction or lead to reduced viability in starved cultures.
    Type of Medium: Electronic Resource
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