ISSN:
1573-7276
Keywords:
cancer
;
colorectal
;
αE-catenin
;
invasion
;
hMSH6
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Transition from an epithelioid (E) to a round (R) morphotype, in the human colon cancer cell line HCT-8, is associated with loss or truncation of αE-catenin and acquisition of invasiveness in organ culture. In E clones, like in parental HCT-8 cells, one allele of the αE-catenin gene (CTNNA1) is mutated. HCT-8 cells have also a “Microsatelite Instability-High” (MSI-H) phenotype presumably due to a mutated hMSH6 gene. Fusion of E type cells doubles the wild type CTNNA1 alleles and prevents the loss of αE-catenin. Introduction of an extra chromosome 2, carrying a wild type hMSH6 gene, restores post-replicative mismatch repair and also prevents the frequent inactivation of the remaining wild type CTNNA1 allele.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006724300022
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