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  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 229 (1971), S. 569-570 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] NIL2 cells3 were grown by standard methods in Eagle's medium with 10% foetal calf serum. Lipids were labelled by growing cells for 2 days in the presence of l-14C-palmitate (1.3 iiCi/ml., 55 pCi/jimol). Cell sheets were washed with phosphate-buffered saline and stored at - 20 C before analysis. ...
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 264 (1976), S. 261-263 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Here we ask the questions first, does transformation of avian cells by agents other than avian sarcoma viruses (ASY) induce a tumour-specific surface antigen (TSSA) hitherto recognised as avian oncornavirus specific; and second, does the additional genetic information supplied by a superinfecting ...
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  • 3
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    Unknown
    Chicago : Periodicals Archive Online (PAO)
    Agricultural History. 60:2 (1986:Spring) 73 
    ISSN: 0002-1482
    Topics: History , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 225 (1970), S. 24-26 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A system is described which has important advantages for the study of viral induced cell transformation. It is based on the discovery of a new virus in a cell line derived from a hamster tumour induced by a murine sarcoma ...
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 203 (1964), S. 1355-1357 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE BHK2I line of diploid hamster fibroblasts is J. used widely for research on viruses and on neoplastic transformation. The purpose of this article is to describe the origin of the cells, to list viruses to which they are susceptible, and to summarize the main characteristics of some variants of ...
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Boundary layer meteorology 66 (1993), S. 237-245 
    ISSN: 1573-1472
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Airplane aerodynamic characteristics correlate aircraft speed with vertical wind velocity, making the time average inappropriate for estimating the ensemble average in airborne eddy-correlation flux computations. The space average, the proper form, is implemented as a time integral by a transformation of variables, which can be interpreted as a ground-speed correction to the time average. The mathematical forms are presented, and the importance of the speed correction is illustrated with airborne data. The computed correction is found to be highly variable, depending on both the turbulent flow encountered and the aircraft used. In general, the speed connection becomes more important as airplane size is reduced. For a small, single-engine Long-EZ airplane, used as an example, the straight time average erred, half the time, by 12%, 10%, 20%, and 15%, respectively, for computed fluxes of momentum, heat, moisture, and CO2. For a much heavier Twin Otter airplane, also used as an example, the straight time average erred, half the time by only 1%. These errors increased with decreasing altitude for the Long-EZ and with increasing altitude for the Twin Otter.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A chromosomally-stable mouse-Chinese hamster hybrid cell line was subjected to five rounds of selection with cytotoxic antisera raised in rabbits against either the parental mouse 3T3 cells or the parental Chinese hamster Wg-1 cells. Routine karyological analysis of clones isolated at each stage of serum selection revealed that treatment with either serum resulted in a limited loss of chromosomes (compared to the untreated hybrid cell cultured in parallel) and that the pattern of chromosome loss could not be correlated with the particular antiserum used for selection. However, more detailed analysis with the SSC-formamide C-banding technique, which identifies chromosomes containing a mouse centromere region, demonstrated that while large-scale chromosome loss was not achieved as a result of antiserum selection, the limited loss of chromosomes did, in fact, reflect a specific depletion of chromosomes in response to treatment with cytotoxic antiserum. Specific chromosomal elimination was shown to occur as early as the first round of antiserum treatment. Antigenic analysis of the serum-selected clones revealed a quantitative decrease in the expression of the species-specific surface antigens selected against, but no qualitative loss of antigens was detected. The results suggest that treatment with cytotoxic antiserum may select for clones that have lost specific chromosomes bearing genes regulating the expression of species-specific surface antigens, rather than for those demonstrating large-scale depletion of chromosomes bearing the corresponding structural genes.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0075-4617
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Die beiden Kohlenwasserstoffe IV und V werden synthetisiert. Bei der Dehydrierung ergibt der erste Benzozethren (VI), während der zweite ein unbeständiges Diradikal liefert. Die Unterschiede werden durch die Möglichkeit einer Kekulé-Struktur mit zwei fixierten Doppelbindungen für VI und der Unmöglichkeit einer Kekulé-Struktur für VII erklärt. Außerdem ergeben sich bedeutsame Unterschiede bei der alternierenden Markierung der Kohlenwasserstoffe VI und VII. Die beiden isomeren Dibenzozethrene XVII und XIX zeigen starke Unterschiede in der Stabilität, die auf die verschiedene Anzahl möglicher aromatischer Sextette zurückgeführt werden.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0091-7419
    Keywords: Life Sciences ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A chromosomally stable mouse-Chinese hamster hybrid cell line was subjected to five rounds of selection with cytotoxic antisera raised in rabbits against either the parental mouse 3T3 cells or the parental Chinese hamster Wg-1 cells. Routine karyological analysis of clones isolated at each stage of serum selection revealed that treatment with either serum resulted in a limited loss of chromosomes (compared to the untreated hybrid cell cultured in parallel) and that the pattern of chromosome loss could not be correlated with the particular antiserum used for selection. However, more detailed analysis with the SSC-formamide C-banding technique, which identifies chromosomes containing a mouse centromere region, demonstrated that while large-scale chromosome loss was not achieved as a result of antiserum selection, the limited loss of chromosomes did, in fact, reflect a specific depletion of chromosomes in response to treatment with cytotoxic antiserum. Specific chromosomal elimination was shown to occur as early as the first round of antiserum treatment. Antigenic analysis of the serum-selected clones revealed a quantitative decrease in the expression of the species-specific surface antigens selected against, but no qualitative loss of antigens was detected. The results suggest that treatment with cytotoxic antiserum may select for clones that have lost specific chromosomes bearing genes regulating the expression of species-specific surface antigens, rather than for those demonstrating large-scale depletion of chromosomes bearing the corresponding structural genes. Some of these chromosomally depleted hybrid cell clones have been used (along with pseudotype viruses containing the genome of vesicular stomatitis virus within the envelope of murine leukemia virus, VSV [MuLV]), to study the mechanisms regulating MuLV replication in Chinese hamster cells. The results indicate that the restriction of MuLV replication in Chinese hamster cells operates at two levels: (a) an inability to adsorb to or penetrate Chinese hamster cells; and (b) an additional intracellular block which is dominant in the mouse-Chinese hamster hybrid cell clones examined. This latter block is presently under study.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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