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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a heat-resistant beef allergen: myoglobin (2004)
    Oxford, UK : Munksgaard International Publishers
    Allergy 59 (2004), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Meat allergy is rarely reported. Most of the described cases are sensitizations to bovine serum albumin.Objective:  The aim of the study was to describe a case of allergy to a new meat allergen and, after its characterization.Methods:  A 35-year-old nonatopic female with allergic episodes after ingestion of several types of meat was studied. Skin tests (prick and prick-to-prick); total and specific immunoglobulin E (IgE) determination; sodium dodecyl sulphate-polyacrylamide gel electrophoresis and specific IgE determination by immunoblotting under different conditions were performed. A 17-kDa allergen was semipurified by ethanol fractionation and its amino-terminal sequence was determined. The existence of specific IgE directed to this protein was studied by immunoblot in 80 atopic patients.Results:  The patient showed specific IgE antibodies to a 17-kDa protein. During the isolation of this allergen it was found that a 70–90% (vol/vol) ethanol concentration was able to purify the protein. The characterization revealed that it was a heat-resistant protein without disulfide bonds. N-terminal amino acid sequence (16 residues) showed identity with myoglobin. The study of specific IgE to this allergen among atopic patients showed that it was recognized by about 1% of the subjects.Conclusions:  We describe a case of meat allergy caused by myoglobin. This is the first described case of monosensitization to this protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.2004.00266.x
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  • 2
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    Bioaerosol Sampling in Modern Agriculture: A Novel Approach for Emerging Pathogen Surveillance? (2016)
    Oxford University Press
    Details
    Publication Date: 2016-07-23
    Description: Background.  Modern agricultural practices create environmental conditions conducive to the emergence of novel pathogens. Current surveillance efforts to assess the burden of emerging pathogens in animal production facilities in China are sparse. In Guangdong Province pig farms, we compared bioaerosol surveillance for influenza A virus to surveillance in oral pig secretions and environmental swab specimens. Methods.  During the 2014 summer and fall/winter seasons, we used 3 sampling techniques to study 5 swine farms weekly for influenza A virus. Samples were molecularly tested for influenza A virus, and positive specimens were further characterized with culture. Risk factors for influenza A virus positivity for each sample type were assessed. Results.  Seventy-one of 354 samples (20.1%) were positive for influenza A virus RNA by real-time reverse-transcription polymerase chain reaction analysis. Influenza A virus positivity in bioaerosol samples was a statistically significant predictor for influenza A virus positivity in pig oral secretion and environmental swab samples. Temperature of 〈20°C was a significant predictor of influenza A virus positivity in bioaerosol samples. Discussions.  Climatic factors and routine animal husbandry practices may increase the risk of human exposure to aerosolized influenza A viruses in swine farms. Data suggest that bioaerosol sampling in pig barns may be a noninvasive and efficient means to conduct surveillance for novel influenza viruses.
    Print ISSN: 0022-1899
    Electronic ISSN: 1537-6613
    Topics: Medicine
    Published by Oxford University Press on behalf of The Infectious Diseases Society of America (IDSA).
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    PAPER CURRENT
  • 3
    Electronic Resource
    Electronic Resource
    Photoemission study of the effect of thermal annealing on Cs2O/Si(111) surfaces (2001)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 90 (2001), S. 3787-3791 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The Cs2O overlayers with different thickness were prepared by simultaneous oxygen–cesium adsorption on Si(111) at room temperature. Photoelectron spectroscopy and work-function measurements have been used to study the Cs2O/Si surfaces as a function of annealing temperature. The results show that the interaction of the Cs2O overlayer with the substrate is weak. The Cs2O species is sensitive to x-ray radiation, forming a new species on the top surface. The Cs2O/Si surfaces exhibit a negative electron affinity with a work-function value of 0.85±0.1 eV until the Cs2O species decomposes completely. After Cs2O disappears, both Cs–O and Si–O bonds are dominant on the surfaces with a work function of about 1.2 eV. For further annealing, the oxygen bonded to cesium gradually transfers to Si due to Cs desorption. The thickness of the SiO2 overlayer formed after Cs desorption is dependent on the mount of oxygen in the Cs2O overlayer. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1405133
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  • 4
    Electronic Resource
    Electronic Resource
    A double hot cathode lateral extraction Penning ion source : Proceedings of the fourth International Conference on Ion Sources (1992)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 63 (1992), S. 2475-2477 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: An improved Penning ion source has been developed. This ion source has two helical filaments at the two ends of the discharge chamber. The design of the filaments is such that the resultant magnetic field distribution, which is a combination of the external field and those due to the two filaments, focuses the electrons from the filaments to the plasma region near to the lateral extraction slit, thus creating a hollow cathode effect. This produces an efficient discharge and a good positive ion output. This ion source can produce various species of ions including the ions of reactive gases with a longer lifetime of filaments than for comparable sources.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1142916
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  • 5
    Electronic Resource
    Electronic Resource
    The effect of the addition of adrenaline to pethidine for patient-controlled epidural analgesia after Caesarean section (1998)
    Oxford : Blackwell Science Ltd
    Anaesthesia 53 (1998), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We have investigated the addition of adrenaline to pethidine for patient-controlled epidural analgesia after elective Caesarean section. In a randomised, double-blind study, patients received patient-controlled epidural analgesia for 24 h using pethidine 5 mgml−1 with adrenaline 5 μgml−1 (adrenaline group, n = 40) or pethidine 5 mgml−1 without adrenaline (plain group, n = 38). Visual analogue scale pain scores at rest and on coughing measured 2 h, 6 h and 24 h after surgery were similar between the two groups. There was a trend towards lower mean total consumption of pethidine in the adrenaline group (231.5 mg; SD 140.5 mg) compared with the plain group (289.5 mg; SD 139.5 mg; p = 0.071). Patients in the adrenaline group had higher visual analogue scale scores for nausea at 2 h and 24 h and higher scores for pruritus at 2 h compared with the plain group. Addition of adrenaline to pethidine for patient-controlled epidural analgesia does not appear to have significant clinical advantages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2044.1998.00521.x
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  • 6
    Electronic Resource
    Electronic Resource
    Patient-controlled pethidine after major upper abdominal surgery: comparison of the epidural and intravenous routes*† (2001)
    Oxford, UK : Blackwell Science Ltd
    Anaesthesia 56 (2001), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We compared epidural (n = 17) and intravenous (n = 20) patient-controlled analgesia (PCA) using pethidine (bolus 10 mg, lockout interval 10 min, 4-h maximum dose 3 mg.kg−1) after total gastrectomy. We found that mean (SD) pethidine consumption in the first 24 h was 33% less in the epidural group [255 (85) mg] than in the intravenous group [379 (129) mg, p = 0.002], although most of this difference occurred in the first 8 h. Plasma concentrations of pethidine were lower at 8 h (p 〈 0.01) in the epidural group, but were similar at 24 h. Pain scores, side-effects, patient satisfaction and patient outcome were similar between groups. Epidural and intravenous pethidine PCA provided similar efficacy after major abdominal surgery, although the epidural route can reduce the amount of pethidine used initially.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2044.2001.1962-4.x
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  • 7
    Electronic Resource
    Electronic Resource
    Addition of adrenaline to pethidine for epidural analgesia after Caesarean section (1997)
    Oxford, UK : Blackwell Science Ltd
    Anaesthesia 52 (1997), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We have investigated the addition of adrenaline to epidural pethidine for postoperative analgesia in 40 patients after Caesarean section. In a randomised, double-blind study, patients received pethidine 25 mg with adrenaline 50 μg (adrenaline group, n = 20) or pethidine 25 mg without adrenaline (plain group, n = 18) epidurally at the first request for postoperative analgesia. The median duration of analgesia was longer in the adrenaline group (196 min; IQR 123–286) compared with the plain group (96 min; IQR 43–113; p = 0.002) and plasma concentrations of pethidine in the first 30 min after injection were lower in the adrenaline group (p = 0.003). Visual analogue scale pain scores in the first 30 min after injection and onset of analgesia, defined by the time for pain scores to decrease by 50%, were similar between groups. Addition of adrenaline to epidural pethidine has advantages for analgesia after Caesarean section.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2044.1997.172-az0306.x
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  • 8
    Electronic Resource
    Electronic Resource
    Incident reporting in acute pain management (1998)
    Oxford : Blackwell Science Ltd
    Anaesthesia 53 (1998), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Incident reporting is an effective tool for continuous quality improvement in clinical practice. A prospective study on voluntary incident reporting in pain management was conducted at a major teaching hospital in Hong Kong. Over a 12-month period, 53 incidents were reported in 1275 patients who received pain relief treatments which were supervised by the acute pain service. The majority of the incidents were first detected by the pain team. The most common incidents involved delivery circuits, delivery pump and drug administration. A large proportion (81.4%) of the incidents were thought to be preventable. Human factors were involved in 41.9% of the patients reported, most commonly associated with unfamiliar technique/inexperience, inattention and inadequate communication. Four patients developed major morbidity of which two were attributed to inadequate analgesia, while three others had major physiological changes without morbidity. Strategies have been formulated to prevent further occurrence of these incidents. We propose that incident reporting is a potentially useful tool in identifying and preventing adverse events in postoperative pain management.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2044.1998.00407.x
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  • 9
    Electronic Resource
    Electronic Resource
    VARIABILITY IN PHYSICOCHEMICAL PROPERTIES OF DRIED WHEYS FROM COMMERCIAL SOURCES (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food quality 13 (1990), S. 0 
    Details
    ISSN: 1745-4557
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Variability in physicochemical characteristics of dried wheys was determined for samples collected from six commercial sources. Eight dried whey samples were collected from each plant over a six month period. The pH in water, percent ash, alkalinity of ash, solubility in water and in acid, and Browning Indexes with and without pronase digestion were determined for each dried whey. Whey proteins were evaluated by gel electrophoresis. Variability of pH in water and of percent ash were low for dried wheys from individual plants, however considerable variability existed in the other characteristics, both between plants and between samples from individual plants. Gel electrophoresis may be useful in evaluating the effects of whey processing and drying on whey proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-4557.1990.tb00012.x
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  • 10
    Electronic Resource
    Electronic Resource
    Pheromones for Monitoring and Control of Stored-Product Insects (1985)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Entomology 30 (1985), S. 257-272 
    Details
    ISSN: 0066-4170
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.en.30.010185.001353
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