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  • 1
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two strains of Streptococcus uberis (UT 888 and UT 366) isolated from cows with clinical mastitis were co-cultured with bovine mammary epithelial cells (MAC-T) with and without laminin, fibrinogen, fibronectin or collagen. Incubation of S. uberis with extracellular matrix proteins (ECMPs) increased adherence to and internalization into MAC-T cells. Both strains of S. uberis exhibited greater adherence when co-cultured in the presence of collagen than with any other ECMP. However, adherence was always higher when strains were co-cultured with ECMP than in medium alone. S. uberis UT 888 adhered better to MAC-T cells than S. uberis UT 366. The influence of ECMPs on bacterial internalization into MAC-T cells was similar to adherence, however, differences among ECMPs were less noticeable. S. uberis UT 888 had a higher internalization index than S. uberis UT 366. It is possible that ECMPs induce or up-regulate proteins that selectively adhere to ECMPs which could serve as a bridge between the eukaryotic cell and the bacterial pathogen that leads to internalization of the ECMP-bound pathogen into the mammary epithelial cell.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 166 (1998), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Three strains of Streptococcus uberis isolated from dairy cows with mastitis were co-cultured with a bovine mammary epithelial cell line (MAC-T) in Dulbecco's modified Eagle's medium without fetal bovine serum. Protein profiles from culture supernatants and bacterial pellets among different treatments were compared by electrophoresis. There were proteins induced or having increased expression in both supernatant and surface-associated samples from S. uberis co-cultured with MAC-T cells. Some of these proteins were recognized by antibodies in serum obtained from a cow infected by S. uberis. In supernatant samples, there were two distinct protein bands at 35 and 36.8 kDa for all three strains of S. uberis co-cultured with MAC-T cells. These two bands were absent when bacterial protein synthesis was inhibited by chloramphenicol. This study clearly indicates that bacterial protein expression was regulated in response to co-culture with mammary epithelial cells.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Surface-associated protein expression by Streptococcus uberis was influenced by the presence of collagen, laminin and bovine mammary epithelial cells in the culture medium. After electrophoresis and silver staining, four proteins stained more intensively in samples from S. uberis cultivated with epithelial cells and extracellular matrix components than in samples from S. uberis cultivated alone. Induction of these proteins was more obvious after multiple bacterial passages. The correlation between the phenotype of S. uberis and its potential virulence status as illustrated by an immunoblotting study with sera obtained from infected cows revealed that these proteins are probably expressed in vivo during infection.
    Type of Medium: Electronic Resource
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  • 4
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    Massachusetts Institute of Technology and Woods Hole Oceanographic Institution
    Publication Date: 2022-05-25
    Description: Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Woods Hole Oceanographic Institution February 1980
    Description: The existence of resonant, baroclinic, equatorially-trapped inertia-gravity waves (discovered by Wunsch and Gill (1976)) is confirmed in the mid-Pacific by spectral analysis of long sea level records. The energy of the low-mode inertia-gravity waves is found to decrease toward the meridional boundaries. A simple spectral model, acknowledging the dispersive characteristics of the equatorial waves, adequately reproduces the observed mid-Pacific sea level spectra in the 1-6 day band. Model spectra computed at latitudes outside the equatorial waveguide of the gravest meridional modes suggest the presence of "inertial" peaks in several observed sea level spectra. Resonant, low-mode inertia-gravity waves may also exist in the Indian Ocean. Sea level fluctuations along the Pacific equator are found to have Kelvin wave characteristics in the 35-80 day band, and, in particular, propagation from the western Pacific to the coast of South America is observed. The Kelvin waves are atmospherically-forced in the central- western Pacific and have a computed equivalent depth corresponding to the first-baroc1inic mode. Outside of the equatorial mid-Pacific, a non-static ocean response to air pressure in the 4-6 day band is dominated by a basin-wide, barotropic, planetary mode. The low Q of this mode suggests that the ocean is viscous with respect to large-scale barotropic oscillations. The dynamical components of the observed long-period tides have been isolated for the first time using the "self-consistent" equilibrium tide of Agnew and Farrell (1978). The tides are slightly non-equilibrium with large horizontal scales. The relatively short-scale Rossby modes predicted by Wunsch (1967) are not observed, perhaps because of the poor spatial coverage of the dataset. Considering the low Q of the 4-6 day planetary basin mode, it is suggested that the long-period tides are frictionally-controlled. The 4- and 5-day equatorial inertia-gravity waves, the 35-80 day Kelvin waves and the 4-6 day planetary basin mode are clearly atmospherically forced, and, perhaps surprisingly, they are forced by atmospheric waves that have similar horizontal structures, i.e., 4-5 day Rossby-gravity waves, 40-50 day Kelvin waves and a 5-day global barotropic mode. The surface expressions of these atmospheric waves are determined in order to understand the nature of the oceanic response, e.g., resonant or forced. Much of the information about the surface atmospheric fields that has been collected, including frequency-wavenumber descriptions, awaits an accurate model of the coupling between wind stress and internal ocean waves.
    Description: Monetary support for this research was provided by the National Science Foundation through contract OCE73-0l384. At various times, tuition and living expenses were paid by funds from the NSF contract above, the Office of Naval Research (contract N00014-C-75-029l), the Cecil and Ida Green Professorship in Earth Sciences, the J. P. Luther Educational Fund and by an M.I.T. Educational Tuition Award.
    Keywords: Ocean waves ; Gravity waves ; Ocean-atmosphere interaction ; Tides
    Repository Name: Woods Hole Open Access Server
    Type: Thesis
    Format: application/pdf
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  • 5
    Publication Date: 2021-12-15
    Description: The Joint Task Force, Science Monitoring And Reliable Telecommunications (JTF SMART) Subsea Cables are working to integrate environmental sensors (temperature, pressure, seismic acceleration) into submarine telecommunications cables. This will support climate and ocean observation, sea level monitoring, observations of Earth structure, tsunami and earthquake early warning and disaster risk reduction. Recent advances include regional SMART pilot systems that are the initial steps to trans-ocean and global implementation. Building on the OceanObs’19 conference and community white paper (doi:10.3389/fmars.2019.00424), this paper presents an overview of the initiative and a description of ongoing projects including: InSea wet demonstration project off Sicily; Vanuatu and New Caledonia; Indonesia; CAM-2 triangle system connecting Lisbon, Azores and Madeira; New Zealand; and Antarctica. In addition to the diverse scientific and societal benefits, the telecommunications industry mission of societal connectivity will also benefit because environmental awareness improves both individual cable system integrity and the resilience of the overall global communications network.
    Description: Published
    Description: San Diego – Porto
    Description: 8T. Sismologia in tempo reale e Early Warning Sismico e da Tsunami
    Keywords: ocean observing ; ocean observatories ; blue economy ; disaster risk reduction ; climate observing ; smart cable
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: Conference paper
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  • 6
    Publication Date: 2021-12-15
    Description: JTF SMART Subsea Cables (Joint Task Force, Science Monitoring And Reliable Telecommunications, 1) is working to integrate environmental sensors for ocean bottom temperature, pressure and seismic acceleration into submarine telecommunications cables. The purpose of SMART Cables is supporting climate and ocean observation, sea level monitoring, observations of Earth structure, and tsunami and earthquake early warning and disaster risk reduction. Recent advances include regional SMART pilot systems that are the first steps to transocean and global implementation. Building on the OceanObs’19 conference and community white paper (2), an overview and description of the status of ongoing projects will include: The InSea wet demonstration project off Sicily at the EMSO Western Ionian Facility; Gondwana-3 connecting New Caledonia and Vanuatu; Indonesia’s Makassar Strait systems working toward systems for the Sumatra-Java megathrust zone; and the CAM-2 triangle system connecting Lisbon, Azores and Madeira. Observing system design studies are elaborated for these and other regions, e. g., the Pacific. Funding reflects a blend of government, development bank, and commercial contributions. In addition to notable scientific and societal benefits, the Telecom mission of societal connectivity will benefit as well, as environmental awareness improves both individual cable system integrity as well as that of the overall global communications network
    Description: Published
    Description: virtual
    Description: 3A. Geofisica marina e osservazioni multiparametriche a fondo mare
    Keywords: ocean observing ; Seismic instruments and networks ; smart cable
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: Oral presentation
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  • 7
    Publication Date: 2022-02-10
    Description: The Joint Task Force, Science Monitoring And Reliable Telecommunications (JTF SMART) Subsea Cables, is working to integrate environmental sensors for ocean bottom temperature, pressure, and seismic acceleration into submarine telecommunications cables. The purpose of SMART Cables is to support climate and ocean observation, sea level monitoring, observations of Earth structure, and tsunami and earthquake early warning and disaster risk reduction, including hazard quantification. Recent advances include regional SMART pilot systems that are the first steps to trans-ocean and global implementation. Examples of pilots include: InSEA wet demonstration project off Sicily at the European Multidisciplinary Seafloor and water column Observatory Western Ionian Facility; New Caledonia and Vanuatu; French Polynesia Natitua South system connecting Tahiti to Tubaui to the south; Indonesia starting with short pilot systems working toward systems for the Sumatra-Java megathrust zone; and the CAM-2 ring system connecting Lisbon, Azores, and Madeira. This paper describes observing system simulations for these and other regions. Funding reflects a blend of government, development bank, philanthropic foundation, and commercial contributions. In addition to notable scientific and societal benefits, the telecommunications enterprise’s mission of global connectivity will benefit directly, as environmental awareness improves both the integrity of individual cable systems as well as the resilience of the overall global communications network. SMART cables support the outcomes of a predicted, safe, and transparent ocean as envisioned by the UN Decade of Ocean Science for Sustainable Development and the Blue Economy. As a continuation of the OceanObs’19 conference and community white paper (Howe et al., 2019, doi: 10.3389/fmars.2019.00424), an overview of the SMART programme and a description of the status of ongoing projects are given.
    Description: Published
    Description: 775544
    Description: 3A. Geofisica marina e osservazioni multiparametriche a fondo mare
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 8
    Publication Date: 2022-05-26
    Description: Author Posting. © American Geophysical Union, 2019. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research-Oceans 124(3), (2019):2072-2087, doi:10.1029/2018JC014586.
    Description: Infragravity waves have received the least study of any class of waves in the deep ocean. This paper analyzes a 389‐day‐long deep ocean pressure record from the Hawaii Ocean Mixing Experiment for the presence of narrowband (≲2 μHz) components and nonstationarity over 400–4,000 μHz using a combination of fitting a mixture noncentral/central χ2 model to spectral estimates, high‐resolution multitaper spectral estimation, and computation of the offset coherence between distinct frequencies for a given data segment. In the frequency band 400–1,000 μHz there is a noncentral fraction of 0.67 ± 0.07 that decreases with increasing frequency. Evidence is presented for the presence of tidal harmonics in the data over the 400‐ to 1,400‐μHz bands. Above ~2,000 μHz the noncentral fraction rises with frequency, comprising about one third of the spectral estimates over 3,000–4,000 μHz. The power spectrum exhibits frequent narrowband peaks at 6–11 standard deviations above the noise level. The widths of the peaks correspond to a Q of at least 1,000, vastly exceeding that of any oceanic or atmospheric process. The offset coherence shows that the spectral peaks have substantial (p = 0.99–0.9999) interfrequency correlation, both locally and between distinct peaks within a given analysis band. Many of the peak frequencies correspond to the known values for solar pressure modes that have previously been observed in solar wind and terrestrial data, while others are the result of nonstationarity that distributes power across frequency. Overall, this paper documents the existence of two previously unrecognized sources of infragravity wave variability in the deep ocean.
    Description: This work was supported at WHOI by an Independent Research and Development award and the Walter A. and Hope Noyes Smith Chair for Excellence in Oceanography. At the University of Hawaii, Martin Guiles provided a number of consequential data analyses, and work was supported by NSF‐OCE1460022. D. J. T. acknowledges support from Queen's University and NSERC. The data used in this study are available from the supporting information.
    Description: 2019-08-20
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 9
    Publication Date: 2022-05-26
    Description: Author Posting. © The Oceanography Society, 2014. This article is posted here by permission of The Oceanography Society for personal use, not for redistribution. The definitive version was published in Oceanography 27, no. 2 (2014): 138-150, doi:10.5670/oceanog.2014.49.
    Description: Increasing public awareness that the Cascadia subduction zone in the Pacific Northwest is capable of great earthquakes (magnitude 9 and greater) motivates the Cascadia Initiative, an ambitious onshore/offshore seismic and geodetic experiment that takes advantage of an amphibious array to study questions ranging from megathrust earthquakes, to volcanic arc structure, to the formation, deformation and hydration of the Juan De Fuca and Gorda Plates. Here, we provide an overview of the Cascadia Initiative, including its primary science objectives, its experimental design and implementation, and a preview of how the resulting data are being used by a diverse and growing scientific community. The Cascadia Initiative also exemplifies how new technology and community-based experiments are opening up frontiers for marine science. The new technology—shielded ocean bottom seismometers—is allowing more routine investigation of the source zone of megathrust earthquakes, which almost exclusively lies offshore and in shallow water. The Cascadia Initiative offers opportunities and accompanying challenges to a rapidly expanding community of those who use ocean bottom seismic data.
    Description: The Cascadia Initiative is supported by the National Science Foundation; the CIET is supported under grants OCE- 1139701, OCE-1238023, OCE‐1342503, OCE-1407821, and OCE-1427663 to the University of Oregon.
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
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  • 10
    Publication Date: 2022-11-09
    Description: The Joint Task Force, Science Monitoring And Reliable Telecommunications (SMART) Subsea Cables is working to integrate environmental sensors (temperature, pressure, seismic acceleration) into submarine telecommunications cables. This will support climate and ocean observation, sea-level monitoring, observations of Earth structure, tsunami and earthquake early warning, and disaster risk reduction. Recent advances include regional SMART pilot systems that are the initial steps to trans-ocean and global implementation. Building on the OceanObs'19conference and community white paper (https://doi.org/10.3389/fmars.2019.00424), this paper presents an overview of the initiative and a description of ongoing projects including: InSea wet demonstration project off Sicily; Vanuatu and New Caledonia; Indonesia; CAM-2 ring system connecting the Portuguese mainland, Azores, and Madeira; New Zealand; and Antarctica. In addition to the diverse scientific and societal benefits, the telecommunications industry's mission of societal connectivity will also benefit because environmental awareness improves both individual cable system integrity and the resilience of the overall global communications network.
    Description: Published
    Description: 13-25
    Description: 3A. Geofisica marina e osservazioni multiparametriche a fondo mare
    Description: JCR Journal
    Keywords: SMART sensors; earthquake early warning; seafloor sensing; telecommunication cables; tsunami detection ; SMART cables
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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