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  • 1
    Electronic Resource
    Electronic Resource
    The effect of carbon dioxide on the growth kinetics of fructose-limited chemostat cultures of Acetobacterium woodii DSM 1030 (1990)
    Springer
    Archives of microbiology 154 (1990), S. 5-11 
    Details
    ISSN: 1432-072X
    Keywords: Acetobacterium woodii ; Fructose growth yields ; Chemostat culture ; Yeast extract ; Carbon dioxide ; YATP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth of the anaerobic acetogenic bacterium Acetobacterium woodii DSM 1030 was investigated in fructose-limited chemostat cultures. A defined medium was developed which contained fructose, mineral salts, cysteine · HCl and Ca pantothenate (1 mg · 1−1) supplied in a vitamin supplement. Growth at high dilution rates was dependent on the presence of CO2 in the gas phase. The μ max was found to be 0.16 h−1 and the fructose maintenance requirement was 0.1 to 0.13 mmol fructose · (g dry wt)−1 · h−1. A growth yield of 61 g dry wt · (mol fructose)−1, corrected for the cell maintenance requirement and for incorporation of fructose carbon into cell biomass, was determined from the fructose consumption. A corresponding growth yield of 69 g dry wt · (mol fructose)−1 was calculated from the acetate production assuming that fructose fermentation was homoacetogenic. A YATP of 12.2 to 13.8 g dry wt · (mol ATP)−1 was calculated from these growth yields using a value of 5 mol ATP · (mol fructose)−1 as an estimate of the amount of ATP synthesised from fructose fermentation. The addition of yeast extract (0.5 g · 1−1) to the medium did not influence the μ max or cell yield. After prolonged growth under fructose-limited conditions the requirement of the culture for CO2 in the gas phase was reduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249170
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dibutylchloromethyltin chloride, a potent inhibitor of electron transport in plant mitochondria (1980)
    Springer
    Journal of bioenergetics and biomembranes 12 (1980), S. 309-323 
    Details
    ISSN: 1573-6881
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Dibutylchloromethyl tin chloride (DBCT) inhibits coupled and uncoupled respiration of mitochondria from potato tubers, cauliflower florets and etiolated mung bean hypocotyls with succinate andl-malate but not with external NADH or TMPD/ascorbate as substrates. Using potato and cauliflower mitochondria, DBCT at 200 pmole/mg of protein gives complete inhibition only in KCl-based media and at pH 6.8. DBCT has no effect on the internal pH of mung bean mitochondria, but does cause a decrease in the membrane potential. Electron transport through the alternative oxidase is not inhibited, neither is the ATP-synthase system. DBCT appears to interact with the functionally-distinct pool of ubiquinone associated with the oxidation of succinate andl-malate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00744691
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    Paper (German National Licenses)
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