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  • 1
    Online Resource
    Online Resource
    Microbial Growth on C1 Compounds : Proceedings of the 8th International Symposium on Microbial Growth on C1 Compounds, Held in San Diego, U. S. A. , 27 August - 1 September 1995. (1996)
    Dordrecht :Springer Netherlands,
    Details
    Keywords: Methylotrophic microorganisms-Congresses. ; Electronic books.
    Description / Table of Contents: Proceedings of the 8th International Symposium on Microbial Growth on C1 Compounds, held in San Diego, U.S.A., 27 August-1 September 1995.
    Type of Medium: Online Resource
    Pages: 1 online resource (371 pages)
    Edition: 1st ed.
    ISBN: 9789400902138
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=6496114
    Language: English
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    GEOMAR EBL
  • 2
    Electronic Resource
    Electronic Resource
    HspR is a global negative regulator of heat shock gene expression in Deinococcus radiodurans (2005)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 55 (2005), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The HspR protein functions as a negative regulator of chaperone and protease gene expression in a diversity of bacteria. Here we have identified, cloned and deleted the Deinococcus radiodurans HspR homologue, DR0934. ΔhspR mutants exhibit moderate growth defects when shifted to mild heat shock temperatures, but are severely impaired for survival at 48°C. Using quantitative reverse transcription polymerase chain reaction and global transcriptional analysis, we have identified 14 genes that are derepressed in the absence of stress in the ΔhspR background, 11 of which encode predicted chaperones and proteases, including dnaKJgrpE, ftsH, lonB, hsp20 and clpB. Promoter mapping indicated that the transcription of these genes initiates from a promoter bearing a σ70-type consensus, and that putative HspR binding sites (HAIR) were present in the 5′-untranslated regions. Electrophoretic mobility shift assays indicated that HspR binds to these promoters at the HAIR site in vitro. These results strongly suggest that DR0934 encodes the HspR-like global negative regulator of D. radiodurans that directly represses chaperone and protease gene expression by binding to the HAIR site in close proximity to promoter regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.2005.04494.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Construction of insertion and deletion mxa mutants of Methylobacterium extorquens AM1 by electroporation (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 166 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Methylobacterium extorquens AM1 is a pink-pigmented facultative methylotroph which is widely used for analyzing pathways of C1 metabolism with biochemical and molecular biological techniques. To facilitate this approach, we have applied a new method to construct insertion or disruption mutants with drug resistance genes by electroporation. By using this method, mutants were obtained in four genes present in the mxa methylotrophy gene cluster for which the functions were unknown, mxaR, mxaS, mxaC and mxaD. These mutants were unable to grow on methanol except the mutant of mxaD, which showed reduced growth on methanol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb13175.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Cloning and characterization of corA, a gene encoding a copper-repressible polypeptide in the type I methanotroph, Methylomicrobium albus BG8 (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 148 (1997), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In an attempt to identify proteins involved in copper transport in the type I methanotroph Methylomicrobium albus BG8, copper-regulated polypeptides were examined. One major copper-repressible membrane polypeptide of approx. 28.500 Da was identified and designated CorA. The gene encoding this polypeptide was isolated and sequenced, and it shared a low identity with a calcium channel protein. An insertion mutation in corA of M. albus BG8 grew very poorly, suggesting that CorA is important for growth of this methanotroph. CorA may be involved in transport of copper and/or other divalent metals ions in M. albus BG8.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1997.tb10284.x
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Sequence and characterization of mxaB, a response regulator involved in regulation of methanol oxidation, and of mxaW, a methanol-regulated gene in Methylobacterium extorquens AM1 (1998)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 160 (1998), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In the facultative serine cycle methylotroph Methylobacterium extorquens AM1, mxaB is required for regulation of methanol oxidation and is located at the end of a large cluster of methylotrophy genes that begins with mxaF. The sequence of mxaB has been obtained and indicates that the gene product is a member of the response regulator family. None of the open reading frames near mxaB showed sequence identity to sensor kinases. Complementation studies suggest a promoter may be located adjacent to mxaB. Another gene (mxaW) is present immediately upstream of mxaF, divergently transcribed from a methanol-inducible promoter. The sequence in the region of mxaW was also obtained. MxaW showed no identity to known proteins. Mutations in mxaW and in an adjacent open reading frame, OrfR, had no effect on growth of M. extorquens AM1 on methanol or other substrates. The MxaW mutant had normal methanol dehydrogenase activity and normal transcription of the mxaF promoter. Therefore, the function of mxaW is unknown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1998.tb12900.x
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Plasmid analysis in pink facultative methylotrophic bacteria using a modified acetone-alkaline hydrolysis method (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 60 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Routine screening of indigenous and recombinant plasmids in pink facultative methylotrophic bacteria has been difficult, time-consuming, and yields variable results. We report a modified alkaline hydrolysis method for rapid plasmid isolation from these organisms that reproducibly results in good yields of closed circular plasmid DNA which can be readily digested with restriction enzymes. This method greatly facilitates direct screening of indigenous and introduced recombinant plasmids in the methylotrophic host strain. We have confirmed earlier findings that the original NCIB wild-type strain of Methylobacterium sp. strain AM1 (NCIB 9133) contains three cryptic plasmids. However, sizing of these plasmids by comparison to standards and by restriction fragment analysis suggests that they are larger than previously reported. We have designated these plasmids pAM1-1 (65 kb), pAM1-2 (40 kb) and pAM1-3 (33 kb). We have also shown that a rifamycin-resistant strain of Methylobacterium sp. strain AM1 used routinely in our laboratory lacks pAM1-2, although no phenotype has been associated with its loss. Finally, we have shoen that another pink facultative methylotroph, Methylobacterium isolate (#YK1), contains three cryptic plasmids of approximately 43, 37 and 22 kb, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03431.x
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Identification of a promoter region for mxaF (moxF) from the type I methanotroph, Methylobacter albus BG8 (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 121 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A fragment of Methylobacter albus BG8 DNA containing mxaF (moxF), the gene encoding the alpha subunit of methanol dehydrogenase, was previously cloned using a fragment of mxaF from Methylobacterium extorquens AM1 as a probe (Stephens et al., J. Bacteriol. (1988) 170, 2063–2069). In this study we identified the 5′ portion of mxaF of M. albus BG8 and sequenced a 1.7-kb region containing the 5′ portion of mxaF and 1.5 kb of upstream DNA. The deduced N-terminal amino acid sequence of mxaF was found to have very high similarity to the previously sequenced mxaF genes. The region directly upstream of mxaF was cloned into a promoter probe vector (pGD500), and promoter activity was demonstrated when the fragment was present in the correct orientation with relation to the reporter gene (lacZ). Using reverse transcriptase, the transcription initiation start site was determined, which was separated from the translation initiation site by 190 nucleotides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07124.x
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  • 8
    Electronic Resource
    Electronic Resource
    Methanotrophic populations in estuarine sediment from Newport Bay, California (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 250 (2005), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Methanotrophic populations have been studied in Newport Bay estuary, Southern California. Environmental clone banks were generated for 16S rRNA genes specific to methanotrophs and for a diagnostic functional gene, pmoA, encoding a conserved subunit of the particulate methane monooxygenase. These clone banks contained sequences specific to types I and II methanotrophs typically found in aquatic environments including freshwater lake and soda lake sediments, aquifers and rice paddies. However, a group of clones that were divergent (93% identity) from known methanotrophic 16S rRNA genes but represented in 16S rRNA gene libraries from other aquatic environments were detected. A group of pmoA sequences divergent (83% identity) from extant methanotrophs and not previously represented in any environmental clone libraries, were also detected. It is concluded that this environment contains significant methanotroph diversity and that some of these may represent novel groups of methanotrophic bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsle.2005.07.016
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Genetics of carbon metabolism in methylotrophic bacteria (1990)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 87 (1990), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The application of genetic techniques to the methylotrophic bacteria has greatly enhanced studies of these important organisms. Two methylotrophic systems have been studied in some detail, the serine cycle for formaldehyde assimilation and the methanol oxidation system. In both cases, genes have been cloned and mapped in Methylobacterium species (facultative serine cycle methanol-utilizers). In addition, methanol oxidation genes have been studied in an autotrophic methanol-utilizer (Paracoccus denitrificans) and three methanotrophs (Methylosporovibrio methanica, Methylomonas albus and Methylomonas sp. A4). Although much remains to be learned in these systems, it is becoming clear that the order of C1 genes has been conserved to some extent in methylotrophic bacteria, and that many C1 genes are loosely clustered on the chromosome. Operons appear to be rare, but some examples have been observed. The extension of genetic approaches to both the obligate and facultative methylotrophs holds much promise for the future in understanding and manipulating the activities of these bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1990.tb04949.x
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    Paper (German National Licenses)
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  • 10
    Electronic Resource
    Electronic Resource
    Characterization of a new marine methylotroph (1984)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 21 (1984), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A methanol-oxidizing bacterium from a marine environment has been isolated and characterized. The bacterium was a Gram-negative rod, capable of growth on methanol and methylamine, but not on multicarbon compounds. It showed a temperature optimum of 30°C, a salt optimum of 0.4% (w/v) and the mol % G + C of its DNA was 46%. Carbon was assimilated via the ribulose monophosphate pathway for formaldehyde fixation during growth on methanol. This bacterium superficially resembled other obligate methylotrophs requiring NaCl reported previously which were designated Methylomonas thalassica. It also appeared similar to many strains of obligate freshwater methylotrophs, except for its NaCl requirement and its lower mol % G + C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1984.tb00219.x
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