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  • 1
    Electronic Resource
    Electronic Resource
    Response of murine spermatocytes to the metaphase-arresting effect of several mitotic arrestants (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1586-1588 
    Details
    ISSN: 1420-9071
    Keywords: Mitotic arrestants ; spermatocyte ; chromosomes ; meiotic metaphase accumulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Three mitotic arrestants, colchicine, Colcemid and vinblastine, were evaluated for their effects on producing meiotic arrest in the laboratory mouse. Colchicine is the most effective and its effect is both dose- and time-dependent. Treatment of mice with 40 mg/kg of colchicine for 3 h resulted in drastic improvement of the yield and quality of M-II figures. This treatment did not appear to increase the aneuploidy frequency in M-II's.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01964819
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Chromosomal damage in sperm of patients surviving Hodgkin's disease following MOPP (nitrogen mustard, vincristine, procarbazine, and prednisone) therapy with and without radiotherapy (1994)
    Springer
    Human genetics 〈Berlin〉 93 (1994), S. 295-299 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Following fusion with hamster eggs, human sperm chromosomes from six Hodgkin's disease patients were analyzed to determine the genotoxic effects of therapy. Each patient had received two to six cycles of MOPP (nitrogen mustard, vincristine, procarbazine, and prednisone), with or without radiotherapy, from 3 to 20 years before the study. A total of 571 cells from the six patients were analyzed; 9.8% of the cells had structural aberrations, and 1.6% were hyperhaploid. Analysis of 5998 metaphases from a control group of 24 male donors revealed only 6.9% of cells with structural aberrations and 0.8% aneuploidy. The increase in hyperhaploidy in the patients was statistically significant. Thus, results of this study suggest that the MOPP regimen, with or without radiotherapy, is capable of causing chromosome abnormalities in the sperm of Hodgkin's disease patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00212026
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    Paper (German National Licenses)
    Fulltext
  • 3
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    Establishment of digital river network control platform in Beijing-Tianjin-Hebei region based on integrated platform (2018)
    Institute of Physics (IOP)
    Details
    Publication Date: 2018-11-06
    Description: The Beijing-Tianjin-Hebei area is the political, economic, cultural, scientific and technological center of our country and an important engine for promoting the economic development. It is the region where human activity of our nation and rest of the world has the biggest intensity to the water cycle disturbance, the biggest in water resources pressure bearing, the greatest difficulty in water resources security guarantee. With the help of 3S visualization technology, knowledge map technology, spatial database technology, it relies on the integrated platform, and the connectivity of the entity water network in Beijing-Tianjin-Hebei area is visualized by information technology, and the digital river network control platform is established. It makes full use of static space to support data with the help of modern monitoring means, and the visualization of monitoring information such as water regime information and reservoir work condition information of Beijing-Tianjin-Hebei area...
    Print ISSN: 1755-1307
    Electronic ISSN: 1755-1315
    Topics: Geography , Geosciences , Physics
    Published by Institute of Physics (IOP)
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  • 4
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    A versatile cis-blocking and trans-activation strategy for ribozyme characterization (2013)
    Oxford University Press
    Details
    Publication Date: 2013-01-20
    Description: Synthetic RNA control devices that use ribozymes as gene-regulatory components have been applied to controlling cellular behaviors in response to environmental signals. Quantitative measurement of the in vitro cleavage rate constants associated with ribozyme-based devices is essential for advancing the molecular design and optimization of this class of gene-regulatory devices. One of the key challenges encountered in ribozyme characterization is the efficient generation of full-length RNA from in vitro transcription reactions, where conditions generally lead to significant ribozyme cleavage. Current methods for generating full-length ribozyme-encoding RNA rely on a trans-blocking strategy, which requires a laborious gel separation and extraction step. Here, we develop a simple two-step gel-free process including cis-blocking and trans-activation steps to support scalable generation of functional full-length ribozyme-encoding RNA. We demonstrate our strategy on various types of natural ribozymes and synthetic ribozyme devices, and the cleavage rate constants obtained for the RNA generated from our strategy are comparable with those generated through traditional methods. We further develop a rapid, label-free ribozyme cleavage assay based on surface plasmon resonance, which allows continuous, real-time monitoring of ribozyme cleavage. The surface plasmon resonance-based characterization assay will complement the versatile cis-blocking and trans-activation strategy to broadly advance our ability to characterize and engineer ribozyme-based devices.
    Keywords: Synthetic Biology and Assembly Cloning
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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  • 5
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    A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity (2012)
    Oxford University Press
    Details
    Publication Date: 2012-11-04
    Description: Recent advances have demonstrated the use of RNA-based control devices to program sophisticated cellular functions; however, the efficiency with which these devices can be quantitatively tailored has limited their broader implementation in cellular networks. Here, we developed a high-efficiency, high-throughput and quantitative two-color fluorescence-activated cell sorting-based screening strategy to support the rapid generation of ribozyme-based control devices with user-specified regulatory activities. The high-efficiency of this screening strategy enabled the isolation of a single functional sequence from a library of over 10 6 variants within two sorting cycles. We demonstrated the versatility of our approach by screening large libraries generated from randomizing individual components within the ribozyme device platform to efficiently isolate new device sequences that exhibit increased in vitro cleavage rates up to 10.5-fold and increased in vivo activation ratios up to 2-fold. We also identified a titratable window within which in vitro cleavage rates and in vivo gene-regulatory activities are correlated, supporting the importance of optimizing RNA device activity directly in the cellular environment. Our two-color fluorescence-activated cell sorting-based screen provides a generalizable strategy for quantitatively tailoring genetic control elements for broader integration within biological networks.
    Keywords: Synthetic Biology and Assembly Cloning
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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  • 6
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    Irregularity in Kπ=8− rotational bands of N=150 isotones (2014)
    American Physical Society (APS)
    Details
    Publication Date: 2014-05-03
    Description: Author(s): X. M. Fu, F. R. Xu, C. F. Jiao, W. Y. Liang, J. C. Pei, and H. L. Liu Motivated by new experimental spectra in transfermium mass region, we have investigated broken-pair high-K multiparticle excited states and their rotational bands for the N=150 isotones around Z=100, by using the configuration-constrained pairing-deformation-frequency self-consistent total-Routhian-... [Phys. Rev. C 89, 054301] Published Fri May 02, 2014
    Keywords: Nuclear Structure
    Print ISSN: 0556-2813
    Electronic ISSN: 1089-490X
    Topics: Physics
    Published by American Physical Society (APS)
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  • 7
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    Manifesting the evolution of eigenstates from quantum billiards to singular billiards in the strongly coupled limit with a truncated basis by using RLC networks (2015)
    American Physical Society (APS)
    Details
    Publication Date: 2015-12-08
    Description: Author(s): P. H. Tuan, H. C. Liang, J. C. Tung, P. Y. Chiang, K. F. Huang, and Y. F. Chen The coupling interaction between the driving source and the RLC network is explored and characterized as the effective impedance. The mathematical form of the derived effective impedance is verified to be identical to the meromorphic function of the singular billiards with a truncated basis. By usin… [Phys. Rev. E 92, 062906] Published Mon Dec 07, 2015
    Keywords: Nonlinear Dynamics and Chaos
    Print ISSN: 1539-3755
    Electronic ISSN: 1550-2376
    Topics: Physics
    Published by American Physical Society (APS)
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