ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The cDNA encoding Peking duck Cu,Zn superoxide dismutase (dSOD) was cloned and sequenced. The recombinant enzyme was overexpressed in Escherichia coli, purified to homogeneity and crystallized using the sitting-drop vapour-diffusion technique. Trigonal crystals of dSOD were obtained at 278 K at low ionic strength and around neutral pH. These crystals belong to space group P3221, with unit-cell parameters a = 124.4, c = 163.5 Å, γ = 120°. The asymmetric unit contains four dimers (eight monomers of Cu,Zn dSOD) and has a 56% solvent content, with a VM of 2.8 Å3 Da−1. On a Rigaku R-AXIS IIc image-plate area-detector system, the crystal diffracted to 2.9 Å. Unusual supermolecular double-helix packing with 922 non-crystallographic symmetry in crystals has been observed in the initial structural analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444901011106
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