Publication Date:
2024-03-15
Description:
Due to the ongoing effects of climate change, phytoplankton are likely to experience enhanced irradiance, more reduced nitrogen, and increased water acidity in the future ocean. Here, we used Thalassiosira pseudonana as a model organism to examine how phytoplankton adjust energy production and expenditure to cope with these multiple, interrelated environmental factors. Following acclimation to a matrix of irradiance, nitrogen source, and CO2 levels, the diatom's energy production and expenditures were quantified and incorporated into an energetic budget to predict how photosynthesis was affected by growth conditions. Increased light intensity and a shift from inline image to inline image led to increased energy generation, through higher rates of light capture at high light and greater investment in photosynthetic proteins when grown on inline image. Secondary energetic expenditures were adjusted modestly at different culture conditions, except that inline image utilization was systematically reduced by increasing pCO2. The subsequent changes in element stoichiometry, biochemical composition, and release of dissolved organic compounds may have important implications for marine biogeochemical cycles. The predicted effects of changing environmental conditions on photosynthesis, made using an energetic budget, were in good agreement with observations at low light, when energy is clearly limiting, but the energetic budget over-predicts the response to inline image at high light, which might be due to relief of energetic limitations and/or increased percentage of inactive photosystem II at high light. Taken together, our study demonstrates that energetic budgets offered significant insight into the response of phytoplankton energy metabolism to the changing environment and did a reasonable job predicting them.
Keywords:
Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; beta-1,3 Gluan, cellular; beta-1,3 Gluan, cellular, standard deviation; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, particulate, per cell; Carbon/Nitrogen ratio; Carbon/Nitrogen ratio, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carbon uptake rate; Carbon uptake rate, standard deviation; Chromista; Electron transport rate, relative; Electron transport rate, relative, standard deviation; Fatty acid content; Fatty acids, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gene expression (incl. proteomics); Glycolic acid, standard deviation; Glycolic acid per cell; Growth/Morphology; Growth rate; Growth rate, standard deviation; Irradiance; Laboratory experiment; Laboratory strains; Light; Macro-nutrients; Maximal electron transport rate, relative; Maximal electron transport rate, relative, standard deviation; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; mRNA copy numbers ratio; mRNA copy numbers ratio, standard deviation; mRNA gene expression, relative; mRNA gene expression, relative, standard deviation; Nitrate reductase activity; Nitrate reductase activity, standard deviation; Nitrogen uptake rate; Nitrogen uptake rate, standard deviation; Non photochemical quenching; Non photochemical quenching, standard deviation; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Ochrophyta; Other metabolic rates; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Particulate organic carbon content per cell, standard deviation; Particulate organic nitrogen per cell; Particulate organic nitrogen per cell, standard deviation; Pelagos; pH; pH, standard deviation; Photochemical quenching; Photochemical quenching, standard deviation; Phytoplankton; Primary production/Photosynthesis; Protein per cell; Proteins, standard deviation; PsbA expression, standard deviation; PsbA expression per cell; Registration number of species; Salinity; Single species; Species; Spectrophotometric; Temperature, water; Temperature, water, standard deviation; Thalassiosira pseudonana; Treatment; Type; Uniform resource locator/link to reference
Type:
Dataset
Format:
text/tab-separated-values, 552 data points
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