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  • 1
    Electronic Resource
    Electronic Resource
    Chromosome doubling in Tripsacum: the production of artificial, sexual tetraploid plants (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A collection of embryogenic diploid calli of Tripsacum was established and treated with colchicine to induce chromosome doubling. Sections containing duplicated cells in calli were identified using flow cytometry and ploidy level was determined in the regenerated plantlets. Tetraploid plants from several origins were obtained. In contrast to wild polyploid plants, which show apomictic development, the regenerated tetraploid plants reproduced sexually. By hybridizing these plants with wild tetraploid apomicts, various populations were established; these will allow a study of the inheritance of apomixis in Tripsacum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00798.x
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  • 2
    Electronic Resource
    Electronic Resource
    Townsend Ionization Constants in n -Alkanes (1960)
    [s.l.] : Nature Publishing Group
    Nature 188 (1960), S. 219-220 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Crowe, Bragg and Devins4 have, on theoretical grounds, related the ionization coefficient, a, to pressure, p, field, E, and to molecular structure through the relationships : alp = Af(Elp) exp(-BP/E) B =(1) A is a constant for a given gas, ei is the ionization potential, q the Ramsauer cross-sect ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/188219a0
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  • 3
    Electronic Resource
    Electronic Resource
    Ionization and Attachment Coefficients in n -Alkyl Chloride Gases (1960)
    [s.l.] : Nature Publishing Group
    Nature 187 (1960), S. 409-410 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The gases used in this work are commercially available products which, when necessary, were purified by vapour phase chromatography. We estimate the final purity of each gas to be greater than 99 per cent. The essential part of the experimental apparatus consists of a pair of plane, parallel ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/187409a0
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of the apomictic mode of reproduction in maize-Tripsacum hybrids using maize RFLP markers (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1198-1203 
    Details
    ISSN: 1432-2242
    Keywords: Diplospory ; RFLP ; Bulk-segregant analysis ; Genome similarity ; Intergeneric hybrids ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyploid plants in the genus Tripsacum, a wild relative of maize, reproduce through gametophytic apomixis of the diplosporous type, an asexual mode of reproduction through seed. Moving gene(s) responsible for the apomictic trait into crop plants would open new areas in plant breeding and agriculture. Efforts to transfer apomixis from Tripsacum into maize at CIMMYT resulted in numerou intergeneric F1 hybrids obtained from various Tripsacum species. A bulk-segregant analysis was carried out to identify molecular markers linked to diplospory in T. dactyloides. This was possible because of numerous genome similarities among related species in the Andropogoneae. On the basis of maize RFLP probes, three restriction fragments co-segregating with diplospory were identified in one maize-Tripsacum dactyloides F1 population that segregated 1∶1 for the mode of reproduction. The markers were also found to be linked in the maize RFLP map, on the distal end of the long arm of chromosome 6. These results support a simple inheritance of diplospory in Tripsacum. Manipulation of the mode of reproduction in maize-Tripsacum backcross generations, and implications for the transfer of apomixis into maize, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222943
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic fingerprinting for determining the mode of reproduction in Paspalum notatum, a subtropical apomictic forage grass (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 850-856 
    Details
    ISSN: 1432-2242
    Keywords: Key words Apomixis ; DNA fingerprinting ; Molecular markers ; Paspalum ; Progeny test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Paspalum is an important genus of the family Gramineae that includes several valuable forage grasses. Many of the species are polyploid and either obligate or facultative apomicts. Cyto-embryological observations of several tetraploid genotypes of P. notatum were performed to determine their mode of reproduction. Afterwards, selfed progenies of the genotypes F131, Q3664 and Q4117 were analysed using RFLP and RAPD genetic fingerprints to identify maternal and non-maternal (aberrant) plants, and to establish the degree of apomictic reproduction. Five maize clones and six primers were used for detecting genetic deviations from the maternal profile. Maize clones umc379, umc384 and umc318 and primers OPG10 and OPI4 were the most informative for discriminating between maternal and aberrant individuals within the progenies of F131 and Q3664. The combined results of three RFLP clones or 4–6 RAPD primers were necessary to ascertain the mode of reproduction in plants F131 and Q3664. The results obtained with the RFLP and RAPD markers were in agreement with the cyto-embryological studies in ascertaining the mode and degree of apomictic reproduction. Plant F131 showed a completely sexual reproductive behaviour, Q3664 an elevated expression of sexuality, while Q4117 was highly apomictic. A fingerprint analysis of an outcrossing population, aimed at the identification of hybrid plants, was also performed. Maize clones um318 and umc379 and primers OPC2 and OPC9 were used. The presence of specific bands belonging to the male parent permitted a rapid and easy detection of hybrids. The methodology described here can be applied both for the characterisation of P. notatum populations and to identify hybrid progenies in Paspalum breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050635
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  • 6
    Electronic Resource
    Electronic Resource
    Identification of a maize linkage group related to apomixis in Brachiaria (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 439-444 
    Details
    ISSN: 1432-2242
    Keywords: Key words Apomixis ; Bulked segregant analysis ; Comparative mapping ; Molecular markers ; Brachiaria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A bulked segregant analysis using RFLPs and RAPDs was carried out to identify molecular markers co-segregating with apomixis in a Brachiaria F1 population. The test population used was a cross between sexual B. ruziziensis R44 and the aposporous apomictic Brachiaria brizantha cv Marandu. The Brachiaria genome was systematically scanned using 61 cDNA and genomic maize clones detecting 65 loci located at 40 cM, on average, one from each other in the maize genome. The finding of a clone that presented a polymorphic band co-segregating with apomixis (umc147) led to the identification of another marker within the same area (umc72). The clones belong to a duplicated linkage group that maps to the distal part of maize chromosome-1 long arm and chromosome-5 short arm. RAPD analysis using 184 primers from Operon sets yielded one more marker (OPC4) significantly linked to the trait mapping the same locus. OPC4 had been previously reported as a potential marker for apospory in Pennisetum. A map of the region was constructed using additional clones that belong to the same maize linkage group. Since that was the only genomic region that presented an apomixis-linked polymorphism our observations support the existence of a single locus directing apospory in Brachiaria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050434
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