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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 75 (1989), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Spleen cells of mice immunized with cis, trans (±) abscisic acid (ABA) conjugated to bovine serum albumin (BSA) were fused with myeloma cells to produce hybridomas. Hybridomas secreting antibodies to cis, trans (+) ABA were detected by enzyme-linked immunoassay and injected into the peritoneal cavity of mice to produce ascites fluid. Antibodies purified by size-exclusion chromatography were linked to cyanogen bromide-activated Sepharose beads and used to prepare immunoaffinity columns (IAC). Semi-purified conifer seedling (Douglas-fir [Pseudotsuga menziesii (Mirb) Franco] lodgepole pine (Pinus contorta Dougl), cedar (Thuja plicata D. Don), and spruce [Picea glauca (Moench) Voss)]) extracts were passed through IAC, washed thoroughly with water and then eluted with methanol. ABA in the methanol eluate was analysed by high performance liquid chromatography with UV detection. UV absorption of extracts before and after purification through IAC indicated an excellent purification of extract by IAC. The identity of the ABA peak was confirmed by gas chromatography-mass spectrometry.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Agroforestry systems 19 (1992), S. 223-232 
    ISSN: 1572-9680
    Keywords: agroforestry ; browsing ; debarking ; sheep ; deer ; Pseudotsuga menziesii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Livestock may provide important service and production functions in agroforestry systems. However, use of livestock in conifer/improved pasture agrosilvopastoral systems is currently limited by concerns about potential damage to trees by livestock. Effects of sheep grazing on Douglas-fir (Pseudotsuga menziesii) trees in two patterns of sheep/pasture/conifer agroforest (cluster and grid plantations) were studied from 4 years after planting (1983) until the first precommercial thinning at age 10 years (1988). Trees averaged over 1 m in height when grazing began in summer 1983. Some browsing of tree lateral branches by sheep occurred regardless of grazing season in 1983–1985. However, the 2 to 10% of current year's lateral branch growth removed by grazing sheep was too low to impact tree growth. Sheep removed the terminal leaders from only 3 to 9% of trees each year during 1983–1985. Most browsing of terminals occurred in the summer when other forages had become mature and were relatively unpalatable to sheep. Less than 13% of agroforest trees were debarked by sheep each year during 1983–1987. By the end of grazing in 1987, less than 8% of agroforest trees had sustained a level of debarking likely to impact future growth (〉50% of tree circumference debarked). Grazing had no discernible effect upon tree diameter or height in any year (P 〉 0.05). Total tree mortality attributable to sheep grazing during 1983–1987 was only 0.9%, including three trees girdled by sheep and two debarked trees which were subsequently attacked by insects. Overall, grazing had no detrimental impact on timber stand growth or mortality.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Agroforestry systems 25 (1994), S. 1-12 
    ISSN: 1572-9680
    Keywords: agroforestry ; grazing ; sheep ; Douglas-fir ; moisture stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant-soil-water relations of a silvopastoral system composed of a Douglas-fir (Pseudotsuga menziesii) timber crop, subterranean clover (Trifolium subterraneum) as a nitrogen-fixing forage, and tall fescue (Festuca arundinacea) as a forage crop were investigated near Corvallis, Oregon, during 1983–1986. Treatments included all possible combinations of two tree-planting patterns (trees planted 2.4 m apart in a grid, and groups of five trees spaced 7.6 m between clusters) and two grazing/understory management systems (agroforests were seeded to subclover and grazed by sheep; forests were unseeded and ungrazed). Mean twig xylem water potential (XWP) for Douglas-fir trees ranged from −0.3 to −1.5 MPa on forest plots and from −0.3 to −1.2 MPa on agroforest sites. Pre-dawn and sunset XWP were more negative for forest than for agroforest plots during dry summer periods. Midday XWP was similar for both agroforest and forest plots on all dates. Soil water content at 50–100 cm depth was greater under agroforest plots as compared to forest plots in 1984, but not in 1985 (unusually dry spring). Average foliage nitrogen content of tree needles was 1.54% vs. 1.43% for agroforests vs. forests, respectively. Our data are consistent with the hypotheses that: (1) grazing of understory vegetation may reduce water stress of trees during dry periods by reducing transpirational water use by the forage plants; and (2) nitrogen-fixing vegetation combined with grazing increases nitrogen uptake of associated trees. However, neither mean foliar N nor average XWP differences experienced by trees in agroforest versus forest plantations were sufficient to have an effect on tree growth. Our data demonstrate that it is possible to produce a second crop (i.e. forage grazed by sheep) in timber plantations without reducing the growth of the main tree crop.
    Type of Medium: Electronic Resource
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