ISSN:
1573-6776
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract A chemical derivative of trypsin, selenotrypsin, was prepared to imitate glutathione peroxidase (GPX) by converting active serine residues in the active site of trypsin into selenocysteines. The strategy for preparation of selenotrypsin contained selective sulfonation by phenylmethanesulfonyl fluoride and nucleophilic substitution by NaHSe. Seleno-trypsin displayed enzyme activity of GPX, which is 150U/(mol. The kinetic properties of selenotrypsin was demonstrated to be similar to that of native GPX. © Rapid Science Ltd. 1998
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005378709179
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