ISSN:
1600-0714
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
The aim of the present study was to investigate epithelial cell proliferation in the linings of odontogenic cysts, including three different subtypes of odontogenic keratocyst (OKC), namely simple (non-recurrent), recurrent and basal cell naevus syndrome (BCNS)-associated lesions. Ki67 immunoreactivity in OKC (simple, n = 10; recurrent, n = 8; syndrome, n = 9), dentigerous cysts (DC, n = 5), radicular cysts (RC, n = 5) and normal oral mucosa (n = 7) was studied using a biotin-streptavidin-peroxidase method on paraffin sections after microwave treatment. Ki67+ epithelial cells were counted manually and related to the length of basement membrane (BM) as determined by TV image analysis. Data were analysed by the Mann-Whitney U test. The number of Ki67+ cells in simple OKC linings (53.1 ± 17.8 cells/ mmBM) was similar to that in oral epithelium (42.5 ± 12.7 cells/mmBM; P〉0.2). However, both contained significantly more Ki67+ cells than DC (3.9 ± 1.3 cells/ mmBM) and RC linings (6.7 ±4.8 cells/mmBM; P〈0.006). The epithelial distribution of Ki67+ cells differed between groups, with the percentage of positive cells in basal layers in OKC linings (7.0 ± 2.1%) being significantly lower than that of oral epithelia (35.9 ± 5.6%), DC (78.4 ± 8.4%) and RC (80.6 ± 7.7%) linings respectively (P〈0.003). Comparison of Ki67 expression within the OKC group revealed no significant difference between simple and recurrent lesions (44.0 ± 13.8 cells/ mmBM; P〉0.3). However, OKC associated with BCNS contained significantly higher numbers of Ki67+ cells (91.8±35.6 cells/mmBM; P〈0.01). There was no difference in epithelial distribution of positive cells between the OKC groups. The results are consistent with OKC having a greater proliferative capacity than DC and RC and that its recurrence is not associated with a subgroup of lesions exhibiting increased proliferation. The increased proliferation in OKC associated with BCNS presumably reflects the underlying genetic defect(s) in this syndrome.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0714.1995.tb01171.x
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