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  • 1
    Online Resource
    Online Resource
    San Diego :Elsevier Science & Technology,
    Keywords: Food--Quality--Congresses. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (369 pages)
    Edition: 1st ed.
    ISBN: 9781483165325
    Language: English
    Note: Front Cover -- Biotechnology and Food Quality -- Copyright Page -- Table of Contents -- Preface -- PART 1: AGRICULTURAL BIOTECHNOLOGY -- Chapter 1. Agricultural Biotechnology: The Benefits for Developed and Developing Countries -- Introduction -- References -- Chapter 2. Food Quality Education -- Introduction -- Scope and Future Role of Food Quality -- Undergraduate Education -- Graduate Education and Research -- Improvement of Agricultural Raw Materials -- Improvement of Processed Foods -- Conclusion -- References -- Chapter 3. Food Quality, Biotechnology, and the Food Company -- Introduction -- Qualify and Profitability -- Demographic and Social Changes -- Consumer Concerns -- Biotechnology and Food Quality -- Conclusion -- References -- Chapter 4. Making Technology Transfer Work -- Introduction -- Federal Laboratories as a Resource -- Tapping Laboratory Technology -- Chapter 5. Biotechnology: Regulatory Considerations -- Coordination of Biotechnology Activities in USDA -- USDA Philosophy on Biotechnology -- Regulation of Meat and Poultry -- Closing -- Chapter 6. Biology: Now Is the Time -- World Change and Biology -- Era of Biology -- Products and Processes of the Era of Biology for Food and Food Quality -- Other Key Factors -- PART 2: GENETIC ENGINEERING AND FOOD QUALITY -- Chapter 7. Characterization and Modification of Maize Storage Proteins -- Introduction -- Results and Discussion -- Localization of Zeins Within Protein Bodies -- Genetic Engineering of Lysine-containing Alpha Zeins -- References -- Chapter 8. Genetic Modification of Traits of Interest to Consumers and Processors -- Introduction -- Cellular Genetics -- Molecular Biology -- Protection of New Developments -- Application of Technology to Food Products -- Conclusion -- References -- Chapter 9. Omega-3 Fatty Acid Improvements in Plants -- Introduction. , PUFA Biosynthesis in Higher Plants -- PUFA Biosynthesis in Diatoms -- Genetic Engineering of Fatty Acid Biosynthesis: A Modest Proposal -- Preconditions for Implementation -- Conclusion -- References -- Chapter 10. Enhancing Meat Quality with Somatotropin (ST): Potentials, Limitations, and Future Research Needs -- Introduction -- Overview of Growth Biology -- Porcine Somatotropin: Historical Information -- Porcine Somatotropin: Beltsville Studies -- Conclusions -- Acknowledgement -- References -- Summary -- PART 3: MOLECULAR COMPONENTS OF FOOD -- Chapter 11. Cell Wall Dynamics -- Introduction -- Experimental -- Results and Discussion -- Acknowledgements -- Abbreviations -- References -- Chapter 12. Prospects for the Use of Genetic Engineering in the Manipulation of Ethylene Biosynthesis and Action in Higher Plants -- Introduction -- Regulation of Ethylene Synthesis -- Mode of Action of Ethylene -- Implication for the Future -- Acknowledgements -- References -- Chapter 13. Tornato Fruit Polygalacturonase: Gene Regulation and Enzyme Function -- Introduction -- Regulation of PG Gene Expression -- Maturation of the PG Protein -- Function of PG -- Conclusions -- Acknowledgements -- References -- Chapter 14. Molecular Interactions of Contractile Proteins -- Introduction -- Muscle Structure -- Myofibril Protein Composition and Protein-Protein Interactions -- Titin and Nebulin -- Changes in Titin Postmortem -- Current Knowledge and Future Prospects -- Acknowledgements -- References -- Chapter 15. Molecular Components of Food: Repartitioning Agents or Hormones -- Introduction -- Mechanism(s) of Action -- Effects on Meat Quality -- Effects on Carcass Quality -- Conclusions -- References -- Summary -- Reference -- PART 4: EVALUATION OF FOOD QUALITY. , Chapter 16. Model Non-Isotopic Hybridization Systems for Detection of Foodborne Bacteria: Preliminary Results and Future Prospects -- Introduction -- Experimental -- Results -- Discussion -- References -- Chapter 17. Use of RFLPs Analysis to Improve Food Quality -- Introduction -- References -- Chapter 18. Hybridoma Technology: The Golden Age and Beyond -- Introduction -- Making Hybrids: Today and Tomorrow -- In vitro Immunization -- Partner Selection -- Catalytic Antibodies -- Specific Antibodies Without Specific Immunization -- Chimeric Antibodies -- Anti-idiotype Manipulation -- What to Expect -- References -- Summary -- PART 5: BIOPROCESSING -- Chapter 19. Bioprocessing of Meats -- Introduction -- Technology of Fermentation -- Topography of Fermentation -- Microbiology of Fermentation -- Protective Cultures -- New Starter Cultures -- References -- Chapter 20. Genetic Modification of Enzymes Used in Food Processing -- Introduction -- Food Enzymes -- Genetic Engineering Technologies -- Clearly Established Enzymes for Known Applications -- New or Modified Enzymes for Known Applications -- New/Modified Enzymes for New/Unknown Applications -- Acknowledgements -- References -- Chapter 21. Impact of Biotechnology on Vegetable Processing -- Introduction -- Agricultural Biotechnology Industry -- Food Industry Needs -- Processed Vegetable Crops -- Tomatoes -- Potatoes -- Discussion -- Summary -- References -- Chapter 22. Genetic Engineering of Lactic Starter Cultures -- Introduction -- Plasmids -- Gene Transfer Systems -- Lactose Metabolism -- Proteinase Activity -- Construction of Strains for Accelerated Cheese Ripening -- Production of Antagonistic Proteins -- Bacteriophage Resistance -- Exopolymer Production -- Vector Construction -- Summary -- References. , Chapter 23. Production of Food Additives and Food Processing Enzymes by Recombinant DNA Technology -- Introduction -- Cecropin, an Antimicrobial Peptide -- Pectic Enzymes for Food Processing -- Concluding Remarks -- References -- Summary.
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  • 2
    Online Resource
    Online Resource
    San Diego :Elsevier Science & Technology,
    Keywords: Plant genetic engineering. ; Plant biotechnology. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (448 pages)
    Edition: 1st ed.
    ISBN: 9781483192604
    Language: English
    Note: Front Cover -- Plant Biotechnology -- Copyright Page -- Table of Contents -- CONTRIBUTORS -- PREFACE -- PART I: Basic Techniques in Plant Biotechnology -- CHAPTER1. Vectors for Gene Transfer in Higher Plants -- 1.1 AGROBACTERIUM-MEDIATED TRANSFORMATION -- 1.2 AGROBACTERIUM VECTORS -- 1.3 USES OF AGROBACTERIUM VECTORS -- 1.4 HOST RANGE OF AGROBACTERIUM -- 1.5 ALTERNATIVE DNA TRANSFER METHODS -- 1.6 CONCLUSIONS -- REFERENCES -- CHAPTER 2. Methods for Transforming Plant Cells -- 2.1 TRANSFORMATION OF DICOTYLEDENOUS PLANTS -- 2.2 TRANSFORMATION OF MONOCOTYLEDENOUS PLANTS -- 2.3 CONCLUSIONS AND FUTURE PROSPECTS -- ADDENDUM -- REFERENCES -- CHAPTER 3. Techniques in Plant Cell and Tissue Culture -- 3.1 CLONAL PROPAGATION -- 3.2 SOMACLONAL VARIATION -- 3.3 GAMETOCLONAL VARIATION -- 3.4 IN VITRO CELL SELECTION-MUTANT ISOLATION -- 3.5 PROTOPLAST FUSION -- 3.6 SYNTHESIS OF SECONDARY PRODUCTS -- 3.7 CONCLUDING REMARKS -- REFERENCES -- CHAPTER 4. Selected Topics in the Genetic Manipulation of the Nuclear Genome -- 4.1 CHROMOSOME TRANSFER -- 4.2 MICROCELL TRANSFER -- 4.3 MICROINJECTION -- 4.4 CONCLUSION -- REFERENCES -- CHAPTER 5. Regulation and Expression of Plant Genes in Microorganisms -- 5.1 RECOGNITION OF PLANT SEQUENCES THAT FUNCTION AS PROMOTERS OF TRANSCRIPTION IN MICROORGANISMS -- 5.2 INCREASING THE RATE OF TRANSCRIPTION OF CLONED GENES -- 5.3 TRANSLATIONAL FEATURES OF PLANT GENE EXPRESSION AND REGULATION -- 5.4 RECOGNITION OF PLANT SIGNAL PEPTIDES -- 5.5 ASSEMBLY OF MULTISUBUNIT PLANT PROTEINS -- 5.6 PRODUCTION OF PROTEINS FOR COMMERCIAL APPLICATIONS AND ANALYTICAL STUDIES -- 5.7 SYNTHESIS OF PLANT PROTEINS FOR SCREENING AND CLONE IDENTIFICATION -- 5.8 COMPLEMENTATION OF BACTERIAL MUTATIONS -- 5.9 PROTEIN STABILITY, SOLUBILITY, AND ACCUMULATION -- 5.10 EXPRESSION IN OTHER BACTERIAL SPECIES -- REFERENCES. , PART II: Regulation of Gene Expression in Plants -- CHAPTER 6. The Molecular Architecture of Plant Genes and Their Regulation -- 6.1 THE ELEMENTS OF PRIMARY STRUCTURE -- 6.2 THE ANALYSIS OF PRIMARY STRUCTURE -- 6.3 FUNCTIONAL ORGANIZATION OF GENES -- 6.4 THE USE OF COMPUTERS IN STRUCTURAL ANALYSES -- 6.5 STORAGE PROTEIN GENES -- 6.6 LIGHT-INDUCED GENES -- 6.7 STRESS-INDUCED GENES -- 6.8 NODULATION GENES -- 6.9 HOUSEKEEPING GENES -- 6.10 CONCLUSION -- REFERENCES -- CHAPTER 7. Induction, Commitment, and Progression of Plant Embryogenesis -- 7.1 DEVELOPMENTAL BIOLOGY OF EMBRYOGENESIS -- 7.2 SOMATIC EMBRYOGENESIS -- 7.3 GENE EXPRESSION IN CARROT CULTURE -- 7.4 IMMUNOLOGICAL APPROACH TO THE IDENTIFICATION OF DEVELOPMENTALLY REGULATED GENES -- 7.5 CONCLUSION -- REFERENCES -- CHAPTER 8. Photoregulation of Gene Expression in Plants -- 8.1 EFFECTS OF LIGHT ON rRNA GENE EXPRESSION -- 8.2 EFFECTS OF LIGHT ON TRANSCRIPT ABUNDANCE -- 8.3 EFFECTS OF PLANT HORMONES -- 8.4 CHLOROPLAST TRANSCRIPT ACCUMULATION -- 8.5 PLASTID DEVELOPMENT AND NUCLEAR GENE EXPRESSION -- 8.6 GENE TRANSFER EXPERIMENTS -- 8.7 TRANS-ACTING FACTORS AND TRANSCRIPTION IN VITRO -- 8.8 CONCLUSION -- REFERENCES -- CHAPTER 9. Hormonal and Stress Regulation of Gene Expression in Cereal Aleurone Layers -- 9.1 THE CEREAL ALEURONE LAYERS -- 9.2 EFFECT OF GIBBERELLINS ON GENE EXPRESSION -- 9.3 EFFECT OF ABA ON GENE EXPRESSION -- 9.4 SUMMARY AND PERSPECTIVE -- REFERENCES -- CHAPTER 10. Auxin-Regulated Gene Expression in Plants -- 10.1 ENHANCEMENT OF SPECIFIC TRANSLATIONAL PRODUCTS BY IAA IN PEA TISSUE -- 10.2 ISOLATION OF DNA SEQUENCES COMPLEMENTARY TO SOME IAA-REGULATED mRNAS IN PEA -- 10.3 CHARACTERIZATION OF THE HORMONAL RESPONSE -- 10.4 DOSE RESPONSE CURVE -- 10.5 MODEL FOR REGULATION OF THE AUXIN GENES -- 10.6 CONCLUSIONS AND FUTURE DIRECTIONS -- REFERENCES. , CHAPTER 11. Cytokinin-modulated Macromolecular Synthesis and Gene Expression -- 11.1 ACTIVE FORMS OF CYTOKININS -- 11.2 CYTOKININ-REGULATED SYNTHESIS OF MACROMOLECULES -- 11.3 THE COMPLEX NATURE OF CYTOKININ-REGULATED GENE EXPRESSION -- 11.4 REGULATION OF GENE EXPRESSION BY COMBINATIONS OF HORMONES -- 11.5 ENHANCEMENT OF LIGHT-REGULATED GENE EXPRESSION BY CYTOKININ -- 11.6 CYTOKININ-BINDING MOLECULES -- 11.7 CONCLUDING REMARKS -- REFERENCES -- CHAPTER 12. Organization and Expression of Genes for Photosynthetic Pigments-Protein Complexes in Photosynthetic Bacteria -- 12.1 METABOLIC VERSATILITY -- 12.2 PHOTOSYNTHETIC APPARATUS -- 12.3 ORGANIZATION OF GENES CODING FOR LH, RC, AND PIGMENT BIOSYNTHETIC ENZYMES AND CYTOCHROMES -- 12.4 REGULATION OF EXPRESSION OF THE GENES CODING FOR LH, RC, BCHL, AND CRT BIOSYNTHESIS -- 12.5 CONCLUSION -- REFERENCES -- PART III: Prospects for Manipulation of Chloroplast Genomes -- CHAPTER 13. Organization and Expression of the Nicotiana Chloroplast Genome -- 13.1 CHLOROPLAST DNA -- 13.2 GENES FOR RRNAs -- 13.3 GENES FOR TRNAs -- 13.4 GENES FOR STROMAL POLYPEPTIDES -- 13.5 GENES FOR THYLAKOID POLYPEPTIDES -- 13.6 GENE EXPRESSION -- 13.7 CONCLUSIONS -- REFERENCES -- CHAPTER 14. Genetic Manipulation of the Chloroplast Genome -- 14.1 CHLOROPLAST TRANSFORMATION BY AGROBACTERIUM TUMEFACIENS -- 14.2 THE CHLOROPLAST GENOME -- 14.3 INTRODUCTION OF DNA INTO PLASTIDS -- 14.4 INTEGRATION OF FOREIGN GENES INTO CHLOROPLAST DNA -- 14.5 AUTONOMOUSLY REPLICATING PLASMIDS -- 14.6 SELECTABLE MARKERS FOR CHLOROPLAST TRANSFORMATION -- 14.7 PROSPECTS -- REFERENCES -- CHAPTER 15. A Perspective on the Biotechnology of Ribulose Bisphosphate Carboxylase/Oxygenase -- 15.1 PROPERTIES OF RUBISCO -- 15.2 FIXING RUBISCO -- 15.3 MOLECULAR ANALYSIS OF RUBISCO FUNCTION -- 15.4 CLONING AND EXPRESSION OF HEXADECAMERIC RUBISCO -- 15.5 FIXING PLANTS. , 15.6 SOME CRITICAL RESERVATIONS -- 15.7 ALTERNATIVE BIOLOGICAL STRATEGIES FOR ENHANCING PHOTOSYNTHESIS -- ADDENDUM -- REFERENCES -- CHAPTER 16. Applications of Nucleic Acid Electron Microscopy and In Situ Hybridization Techniques in the Study of Plant Genomes -- 16.1 ELECTRON MICROSCOPY OF ORGANELLE GENOMES -- 16.2 DNA-DNA HETERODUPLEX ANALYSIS -- 16.3 ELECTRON MICROSCOPY OF DNA-RNA HYBRIDS -- 16.4 LOCALIZATION OF DNA REPLICATION INITIATION SITES BY ELECTRON MICROSCOPY -- 16.5 CHROMOSOMAL LOCALIZATION OF CLONED GENES BY IN SITU HYBRIDIZATION -- REFERENCES -- CHAPTER 17. Molecular Evolution of Nicotiana Chloroplast Genomes -- 17.1 CHLOROPLAST GENOMES -- 17.2 COMMONALITY AND DIVERSITY OF NICOTIANA CHLOROPLAST GENOMES -- 17.3 MOLECULAR EVOLUTION OF NICOTIANA CHLOROPLAST GENOMES -- 17.4 CONCLUDING REMARKS -- REFERENCES -- PART IV: Applications of Biotechnology in Plant Systems -- CHAPTER18. Genetic Engineering for Crop Improvement -- 18.1 PLANT TRANSFORMATION -- 18.2 CROP IMPROVEMENT -- 18.3 CONCLUSIONS -- REFERENCES -- INDEX.
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  • 3
    ISSN: 1573-5028
    Keywords: cytokinin ; tumor formation ; isopentenyltransferase gene ; Nicotiana ; genetic tumors ; polymerase chain reaction (PCR) ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The shooty morphology of a nontumorous amphidiploid mutant of Nicotiana glauca Grah. x N. langsdorffii Weinm. was restored by cytokinins, whether exogenously applied or endogenously produced by transformation of the mutant with a transfer DNA (T-DNA) cytokinin-biosynthesis gene (isopentenyltransferase; ipt). Auxins alone did not confer this effect. Similar transformation was not achieved for the parental species. In the case of transformation with the ipt gene, selection of the transformed tissues was based on its hormone-independent growth in the presence of the antibiotic kanamycin. Transformed tissues exhibited a shooty morphology, indistinguishable from that of wildtype genetic tumors N. glauca x N. langsdorffii. This altered phenotype was caused by the presence and constitutive expression of the ipt gene. The insertion and expression of this gene in transformed tissues was confirmed by using the polymerase chain reaction (PCR) technique as well as conventional molecular hybridization analysis. Expression of the ipt gene led to an elevated level of cytokinin in the transformed mutant tissues. This evidence supports the notion that genetic tumors are caused, at least in part, by elevated levels of cytokinin in interspecific hybrids.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 30 (1996), S. 711-722 
    ISSN: 1573-5028
    Keywords: developmental regulation ; gene expression ; gene family ; phenylalanine ammonia-lyase ; tobacco ; wound response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phenylalanine ammonia-lyase (PAL) catalyzes the first reaction in the general phenylpropanoid pathway leading to the production of phenolic compounds with a significant range of biological functions. A PAL gene we designated gPAL1, cloned from tobacco, consists of two exons separated by an intron of 1932 bp. Exon I, 398 bp, and exon II, 1747 bp, together encode a polypeptide of 715 amino acids. A putative TATA box and polyadenylation signal are found 144 bp upstream of the initiation codon and 193 bp downstream from the stop codon, respectively. Using various parts of gPAL1 as probes, genomic Southern blots indicated the presence of a small family of PAL genes in the tobacco genome that can be divided into two distinct subfamilies, one consisting of pal1 and pal2 and another of pal3 and pal4. Comparative genomic blot analysis of progenitor species (Nicotiana tomentosiformis and N. sylvestris) indicated that each species contains one PAL gene from each of the subfamilies, suggesting that pal1 and pal3 (or pal2 and pal4) diverged prior to the evolution of N. tabacum. Expression of the PAL gene family was examined using RNA gel blots. PAL transcript levels were significantly higher in flowers and roots than in leaves and stems of mature plants. PAL transcripts accumulate differentially during flower and leaf maturation in that mRNA levels decline during flower maturation but increase during leaf maturation. In leaves, PAL transcripts rapidly accumulated after wounding.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-5028
    Keywords: Nicotiana ; oxygen evolution ; photosystem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-5028
    Keywords: gene family ; Nicotiana ; oxygen evolution ; photosystem ; polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5028
    Keywords: ATP ; GTP ; protein kinase ; receptor ; rice ; signal transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A receptor-like protein kinase, OsPK10, has been cloned from rice (Oryza sativa). The 2.8 kb cDNA contains an open reading frame capable of encoding a peptide sequence of 824 amino acids. The topological features of the predicted OsPK10 protein include an N-terminal signal peptide, a cysteine-rich extracellular ligand-binding domain, a membrane-spanning segment, and a cytoplasmic domain possessing all the hallmarks of catalytic domains of eukaryotic protein kinases. The cytoplasmic domain was selectively expressed in Escherichia coli and assayed for kinase activity. The results show the protein is capable of autophosphorylation using either ATP or GTP as the phosphate donor. Phosphoamino acid analysis reveals phosphorylation of threonines, consistent with the substrate specificity indicated by sequence motifs in the catalytic core. A single amino acid substitution of Glu for Lys-528 completely abolishes autophosphorylation activity. DNA gel blot analyses suggest that the haploid rice genome contains a single copy of the OsPK10 gene. OsPK10 transcripts appear to be more abundant in shoots than in roots of rice seedlings.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-5028
    Keywords: Nicotiana ; oxygen evolution ; photosystem ; promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 18 (1992), S. 581-584 
    ISSN: 1573-5028
    Keywords: gene family ; polymerase chain reaction ; protein kinases ; signal transduction ; soybean (Glycine max L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this study we report identification of six members of a protein kinase gene family from soybean (Glycine max L.). Two fully degenerate oligonucleotide primers corresponding to two conserved motifs (DLK-PENV and GTHEYLAPE) in the catalytic domains of eukaryotic protein serine/threonine kinases were used in a polymerase chain reaction (PCR) to amplify soybean cDNA. Sequence analysis showed that 28 of the PCR sequences represented six different putative protein serine/threonine kinases. These results not only demonstrate that catalytic domains of protein kinases are highly conserved between plants and other eukaryotes but also suggest that there are multiple genes encoding protein kinases in plants.
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  • 10
    ISSN: 1432-2048
    Keywords: Nicotiana (photosystem II polypeptide) ; Photosystem II polypeptide (inheritance)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Soluble proteins from leaves of Nicotiana glauca Grah., N. langsdorffii Weinm., their reciprocal hybrids and amphiploid hybrid (N. glaucaxN. langsdorffii) were resolved by two-dimensional gel electrophoresis. Among a group of well-resolved polypeptides, in the isoelectric-point range of 5–5.5 and relative-molecular-mass (Mr) range of 18–23 kilodaltons (kDa), species-specific variation was observed. Polypeptides designated “L” and “l” are specific to N. langsdorffii, and “G” and “g” to N. glauca, while “C” is common to both species. Polypeptides “L”, “G” and “C” are localized in the chloroplasts and associated with thylakoid membranes. Polypeptide “L” is more acidic than polypeptide “G”, and both polypeptides have an Mr of 23 kDa. They were isolated from two-dimensional gels and their first 13 N-terminal amino-acid sequences were determined. These were found to be identical to the 13N-terminal amino acids of the photosystem II (PSII) 23-kDa polypeptide from spinach (T. Jansen et al. (1987) FEBS Lett. 216, 234–240) and, except for one change, to those from pea (R. Wales et al. (1989) Plant Molec. Biol., in press). Polypeptides “G” and “L” cross-react with antiserum against the PSII 23-kDa polypeptide from pea. Therefore, polypeptides “G” and “L” are extrinsic PSII 23-kDa polypeptides. They appear jointly and in equal amounts in the reciprocal hybrids. Since chloroplasts in Nicotiana are maternally inherited, these results demonstrate that polypeptides “G” and “L” are encoded by nuclear genes, are polymorphic variants of the PSII 23-kDa polypeptide, and are inherited in a Mendelian manner.
    Type of Medium: Electronic Resource
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